EMFn010 (genetic_marker) Fragaria nubicola

Marker Overview


Name	EMFn010
Genbank ID	AJ639622
Type	SSR
Species	Fragaria nubicola
Germplasm	EMR-601
Source Type	genomic clone
Repeat Motif	TC 17mer
PCR Condition	Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min)
Primer 1	EMFn010.EMFn010F: GATTAATGCCCAGTGATGGAAT
Primer 2	EMFn010.EMFn010R: CACTGTGGAAGTATGTGTTGGG
Primer 3	EMFn010.Forward: GATTAATGCCCAGTGATGGAAT
Primer 4	EMFn010.Reverse (no gttt): CACTGTGGAAGTATGTGTTGGG
Publication	[view all]
Contact	D. Sargent Eric van de Weg
Comment	c: present in one parent only (1:1 segregation), h: heterozyogous (1:3 segregation), d: distorted c markers, y:distorted h markers

Contact

Name

Details

D. Sargent

First name:	Dan
Last name:	Sargent
Institution:	East Malling Research
Address:	New Road, East Malling, Kent ME19 6BJ, UK
Email:	dan.sargent@emr.ac.uk
Phone:	+44 1732 523747

Eric van de Weg

First name:	Eric
Last name:	van de Weg
Title:	researcher
Institution:	Wageningen-UR Plant Breeding, Wageningen University and Research Centre
Address:	P.O. Box 16, 6700 AA Wageningen, The Netherlands
Country:	The Netherlands
Email:	eric.vandeweg@wur.nl
Keywords:	allo-octoploid strawberry

Publications


Year	Publication
2004	Sargent DJ, Davis TM, Tobutt KR, Wilkinson MJ, Battey NH, Simpson DW. A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria. Theoretical and Applied Genetics. 2004 Nov; 109(7):1385-1391.

Germplasm


Stock Name	Type
EMR-601	accession

Map Positions


#	Map Name	Linkage Group	Bin	Position	Locus	MapViewer
1	Strawberry-232x1392-F1	V-4	N/A	22.89	EMFn010-223/240	View
2	Strawberry-232x1392-F1	V-M.4	N/A	4.53	EMFn010-223/240	View
3	Strawberry-232x1392-F1-2015	LGV-4	N/A	37.01	EMFn010-223/240	View
4	Strawberry-232x1392-F1	V-3a	N/A	0.01	EMFn010-193	View
5	Strawberry-232x1392-F1-2015	LGV-3	N/A	17.5	EMFn010-193	View
6	Strawberry-232x1392-F1	V-1	N/A	17.79	EMFn010-200/220	View
7	Strawberry-232x1392-F1-2015	LGV-1	N/A	41.32	EMFn010-200/220	View
8	Fragaria-FVxFN-F2-2010	FG5	N/A	55.89	EMFn010	View
9	Strawberry-HK-F1	LG5A	N/A	41.8	EMFn010	View
10	Strawberry-HK-F1	LG5B	N/A	37.3	EMFn010	View
11	Strawberry-HK-F1	LG5C	N/A	44.8	EMFn010	View
12	Strawberry-HK-F1	LG5D	N/A	47.7	EMFn010	View
13	Strawberry-High_Density_SSR_Map-F1	5A	N/A	56.55	EMFn010	View

Sequence

>EMFn010 ID=EMFn010; Name=EMFn010; organism=Fragaria nubicola; type=genetic_marker; length=191bp
GATTAATGCCCAGTGATGGAATCCAAATTTAAGGAGCGGTGTGGGTGGTT
ATTCATTCATAAGCCTTACTCTTCATTACCCCTCTCTCATCTCTCTCTCT
CTCTCTCTCTCTCTCTCTCTCTCTCTCCATCCATCCATCCATCCTCTCTT
CTCTCTCTCCCTCTCTCTCCCCAACACATACTTCCACAGTG