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Marker Overview
| Name | EMFn121 |
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| Genbank ID | AM051327 |
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| Type | SSR |
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| Species | Fragaria nubicola |
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| Germplasm | EMR-601 |
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| Source Type | genomic clone |
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| Repeat Motif | GT12&GA9
Direct |
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| PCR Condition | Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min) |
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| Primer 1 | EMFn121.EMFn121F: GGTCCCTAAGTCCATCATGC |
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| Primer 2 | EMFn121.EMFn121R: GAGTGGATGCAAACATGAGC |
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| Primer 3 | EMFn121.Forward: GGTCCCTAAGTCCATCATGC |
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| Primer 4 | EMFn121.Reverse (no gttt): GAGTGGATGCAAACATGAGC |
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| Publication | [view all] |
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| Contact | D. Sargent
Eric van de Weg
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| Comment | c: present in one parent only (1:1 segregation), h: heterozyogous (1:3 segregation), d: distorted c markers, y:distorted h markers |
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Publications
| Year | Publication |
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| 2006 | Sargent DJ, Clarke J, Simpson DW, Tobutt KR, Arús P, Monfort A, Vilanova S, Denoyes-Rothan B, Rousseau M, Folta KM, Bassil NV, Battey NH. An enhanced microsatellite map of diploid Fragaria. Theoretical and Applied Genetics. 2006 May; 112(7):1349-1359. |
Sequence
>EMFn121 ID=EMFn121; Name=EMFn121; organism=Fragaria nubicola; type=genetic_marker; length=244bp
GGTCCCTAAGTCCATCATGCGTTTAATTTCAAGTTTGCAATTTCATTATT
GAAATCTCAGCATCTGATACCAATGGCAAGGCAATCCTATGATTAAGCCC
AAGTGGTAGGGATAGGATCTAATACTACCGCACAATTGACAAAAGATTAA
AACCTGTGTCTGCACGCGCGGTGTGTGTGTGTGTGTGTGTGTGTGAGAGA
GAGAGAGAGAGAGCCTAGTATCGAGCTCATGTTTGCATCCACTC
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