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Marker Overview
| Name | EMFv023 |
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| Genbank ID | AJ564181 |
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| Type | SSR |
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| Species | Fragaria vesca |
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| Germplasm | Ruegen |
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| Source Type | genomic clone |
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| Repeat Motif | AG
Direct |
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| PCR Condition | Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min) |
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| Primer 1 | EMFv023.EMFv023F: AATTACCGAGCCTCCCACACTA |
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| Primer 2 | EMFv023.EMFv023R: CAGCGCTAAAGCGGTTGC |
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| Primer 3 | EMFv023.Forward: AATTACCGAGCCTCCCACACTA |
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| Primer 4 | EMFv023.Reverse (no gttt): CAGCGCTAAAGCGGTTGC |
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| Publication | [view all] |
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| Contact | D. Sargent
Eric van de Weg
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Publications
| Year | Publication |
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| 2003 | James CM, Wilson F, Hadonou AM, Tobutt KR.. Isolation and characterization of polymorphic microsatellites in diploid strawberry (Fragaria vesca L.) for mapping, diversity studies and clone identification. Molecular Ecology Notes. 2003; 3:171-173. |
| 2004 | Hadonou AM, Sargent DJ, Wilson F, James CM, Simpson DW. Development of microsatellite markers in Fragaria, their use in genetic diversity analysis, and their potential for genetic linkage mapping. Genome / National Research Council Canada = Génome / Conseil national de recherches Canada. 2004 Jun; 47(3):429-38. |
Germplasm
| Stock Name | Type |
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| Ruegen | accession |
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