EMFv025 (genetic_marker) Fragaria vesca

Marker Overview
NameEMFv025
Genbank IDAJ564183
TypeSSR
SpeciesFragaria vesca
GermplasmRuegen
Source Typegenomic clone
Repeat MotifGCT
PCR ConditionFinal reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min)
Primer 1EMFv025.EMFv025F: CAAAACGGCTTGATGATCTTCAGC
Primer 2EMFv025.EMFv025R: CAGGGCAAGGTTCAGGGTCTAAAT
Publication[view all]
ContactD. Sargent
Contact
NameDetails
D. Sargent
First name:Dan
Last name:Sargent
Institution:East Malling Research
Address:New Road, East Malling, Kent ME19 6BJ, UK
Email:dan.sargent@emr.ac.uk
Phone:+44 1732 523747
Germplasm
Stock NameType
Ruegenaccession
Map Positions
#Map NameLinkage GroupBinPositionLocusMapViewer
1Fragaria-FVxFN-F2-2010FG1N/A46.11EMFv025View
2Strawberry-RVxFB-NIL-2017LG1N/A60.5EMFv025View