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Marker Overview
| Name | EMFv143 |
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| Genbank ID | N/A |
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| Type | SSR |
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| Species | Fragaria vesca |
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| Germplasm | Ruegen |
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| Source Type | genomic clone |
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| Repeat Motif | TC
17mer |
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| PCR Condition | Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min) |
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| Primer 1 | EMFv143.EMFv143F: ACAATTGGAGCGACACCTTC |
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| Primer 2 | EMFv143.EMFv143R: CCGCGGTTTGATGAGTAAAA |
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| Primer 3 | EMFv143.Forward: ACAATTGGAGCGACACCTTC |
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| Primer 4 | EMFv143.Reverse (no gttt): CCGCGGTTTGATGAGTAAAA |
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| Publication | [view all] |
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| Contact | D. Sargent
Eric van de Weg
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| Comment | c: present in one parent only (1:1 segregation), h: heterozyogous (1:3 segregation), d: distorted c markers, y:distorted h markers |
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Germplasm
| Stock Name | Type |
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| Ruegen | accession |
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