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Marker Overview
| Name | EMFvi008 |
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| Genbank ID | AJ564333 |
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| Type | SSR |
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| Species | Fragaria viridis |
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| Germplasm | EMR-903 |
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| Source Type | genomic clone |
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| Repeat Motif | TG
17mer |
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| PCR Condition | Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min) |
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| Primer 1 | EMFvi008.EMFvi008F: TTGTTTGGATAGTGCGTGTTTC |
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| Primer 2 | EMFvi008.EMFvi008R: AGTGCCAGGATCATGTTTTTCT |
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| Primer 3 | EMFvi008.Forward: TTGTTTGGATAGTGCGTGTTTC |
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| Primer 4 | EMFvi008.Reverse (no gttt): AGTGCCAGGATCATGTTTTTCT |
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| Publication | [view all] |
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| Contact | D. Sargent
Eric van de Weg
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Sequence
>EMFvi008 ID=EMFvi008; Name=EMFvi008; organism=Fragaria viridis; type=genetic_marker; length=292bp
TTGTTTGGATAGTGCGTGTTTCACCTGTCTGGATTTTTTCGTTTGAAAGT
AACATGGGGAATCCAAGTGAACGAAGGAAANCAATAAATCTCTTAACTAA
GATGCAAGCCTTAGAATGCCCAAGATGGGATTTATAATCTGAAGTGGACT
GTCTACCTATAGTCCTATTGTGCGAGCTATTGGATGTTGTTATGAATTAA
TGGGATTTTTTTTTTGATTGAATTAATGGGATTTGAACGCTTACTTTGAT
TTTTGTGTGTGTGTGTGTGTAGAAAAACATGATCCTGGCACT
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