EMFvi018 (genetic_marker) Fragaria viridis

Marker Overview


Name	EMFvi018
Genbank ID	AJ564334
Type	SSR
Species	Fragaria viridis
Germplasm	EMR-903
Source Type	genomic clone
Repeat Motif	TC Direct
PCR Condition	Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min)
Primer 1	EMFvi018.EMFvi018F: CAAACATGGAAGGAAAAGAAGG
Primer 2	EMFvi018.EMFvi018R: GTCAGAGAGACCCATCTGAACC
Primer 3	EMFvi018.Forward: CAAACATGGAAGGAAAAGAAGG
Primer 4	EMFvi018.Reverse (no gttt): GTCAGAGAGACCCATCTGAACC
Publication	[view all]
Contact	D. Sargent Eric van de Weg
Comment	c: present in one parent only (1:1 segregation), h: heterozyogous (1:3 segregation), d: distorted c markers, y:distorted h markers

Contact

Name

Details

D. Sargent

First name:	Dan
Last name:	Sargent
Institution:	East Malling Research
Address:	New Road, East Malling, Kent ME19 6BJ, UK
Email:	dan.sargent@emr.ac.uk
Phone:	+44 1732 523747

Eric van de Weg

First name:	Eric
Last name:	van de Weg
Title:	researcher
Institution:	Wageningen-UR Plant Breeding, Wageningen University and Research Centre
Address:	P.O. Box 16, 6700 AA Wageningen, The Netherlands
Country:	The Netherlands
Email:	eric.vandeweg@wur.nl
Keywords:	allo-octoploid strawberry

Publications


Year	Publication
2006	Nier S, Simpson DW, Tobutt KR, Sargent DJ. A genetic linkage map of an inter-specific diploid Fragaria BC1 mapping population and its comparison with the Fragaria reference map (FV x FN). Journal of Horticultural Science and Biotechnology. 2006; 81(4):645-650.
2003	Molecular ecology notes. 2003 Dec., v. 3, no. 4 p. 550-552. 1022669612ISSN 1471-8278

Germplasm


Stock Name	Type
EMR-903	accession

Map Positions


#	Map Name	Linkage Group	Bin	Position	Locus	MapViewer
1	Strawberry-232x1392-F1	V-4	N/A	55.04	EMFvi018-20	View
2	Strawberry-232x1392-F1	V-3b	N/A	20.15	EMFvi018-215	View
3	Strawberry-232x1392-F1-2015	LGV-3	N/A	63.51	EMFvi018-215	View
4	Strawberry-232x1392-F1	V-1	N/A	54.84	EMFvi018-231	View
5	Fragaria-815x903-BC1	V	N/A	54.15	EMFvi018	View
6	Fragaria-FVxFN-F2-2010	FG5	N/A	60.97	EMFvi018	View
7	Strawberry-HK-F1	LG5A	N/A	73.3	EMFvi018	View
8	Strawberry-HK-F1	LG5B	N/A	85.5	EMFvi018	View
9	Strawberry-HK-F1	LG5C	N/A	63.6	EMFvi018	View
10	Strawberry-HK-F1	LG5D	N/A	70.2	EMFvi018	View
11	Strawberry-RVxFB-NIL-2017	LG5	N/A	58	EMFvi018	View
12	Strawberry-232x1392-F1-2015	LGV-1	N/A	64.49	EMFvi018-231h	View
13	Strawberry-232x1392-F1-2015	LGV-4	N/A	66.51	EMFvi018-205	View

Sequence

>EMFvi018 ID=EMFvi018; Name=EMFvi018; organism=Fragaria viridis; type=genetic_marker; length=251bp
CAAACATGGAAGGAAAAGAAGGCAGATGGGTTCGGCTTACAAATAGCACG
TGTTGGTCGTGGTTGGGCTTCAGTTTCAAACACAAACACAGCATCATCAC
TCTGATCCCAGATCCCTGTGTAATATTCTCATCTTCTCTCTCTCTCTCTC
TCTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATCTTTGCGTTTT
GTAGTTGCTGTAACACTGCAGTTGACTTGGGTTCAGATGGGTCTCTCTGA
C