EMFvi038 (genetic_marker) Fragaria viridis

Marker Overview


Name	EMFvi038
Genbank ID	AJ564337
Type	SSR
Species	Fragaria viridis
Germplasm	EMR-903
Source Type	genomic clone
Repeat Motif	TG
PCR Condition	Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min)
Primer 1	EMFvi038.EMFvi038F: GTCACCAATTCCATCAACACTG
Primer 2	EMFvi038.EMFvi038R: TTCATCCAATTCTCAAAGTCCC
Publication	[view all]
Contact	D. Sargent

Contact

Name

Details

D. Sargent

First name:	Dan
Last name:	Sargent
Institution:	East Malling Research
Address:	New Road, East Malling, Kent ME19 6BJ, UK
Email:	dan.sargent@emr.ac.uk
Phone:	+44 1732 523747

Publications


Year	Publication
2006	Nier S, Simpson DW, Tobutt KR, Sargent DJ. A genetic linkage map of an inter-specific diploid Fragaria BC1 mapping population and its comparison with the Fragaria reference map (FV x FN). Journal of Horticultural Science and Biotechnology. 2006; 81(4):645-650.
2003	Molecular ecology notes. 2003 Dec., v. 3, no. 4 p. 550-552. 1022669612ISSN 1471-8278

Germplasm


Stock Name	Type
EMR-903	accession

Map Positions


#	Map Name	Linkage Group	Bin	Position	Locus	MapViewer
1	Fragaria-815x903-BC1	III	N/A	35.49	EMFvi038	View
2	Fragaria-FVxFN-F2-2010	FG3	N/A	39.7	EMFvi038	View
3	Strawberry-High_Density_SSR_Map-F1	3B	N/A	74.12	EMFvi038	View

Sequence

>EMFvi038 ID=EMFvi038; Name=EMFvi038; organism=Fragaria viridis; type=genetic_marker; length=177bp
GTCACCAATTCCATCAACACTGCCATCAAGGTTGGCACTTCAAATTTTAA
TTTTAATTTTTTTTTGTTCTGATTTTTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTATTGGAAGTGAGTTTAGTTTTGGNTTTGNGNTGCAGGAA
AATTGGGGACTTTGAGAATTGGATGAA