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Marker Overview
| Name | FvNES1 |
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| Genbank ID | N/A |
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| Type | gene marker |
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| Species | Fragaria vesca |
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| Source Type | mRNA |
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| Repeat Motif | na |
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| PCR Condition | Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min) |
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| Primer 1 | FvNES1.AAP418: TGCTGATCATAGATCAGATGG |
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| Primer 2 | FvNES1.AAP456: TGCT(C/T)GGTTTCAACGT(G/T)CAT |
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| Primer 3 | FvNES1.Forward: TGCTGATCATAGATCAGATGG |
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| Primer 4 | FvNES1.Reverse (no gttt): TGCT(C/T)GGTTTCAACGT(G/T)CAT |
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| Publication | [view all] |
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| Contact | D. Sargent
Eric van de Weg
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Publications
| Year | Publication |
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| 2004 | Sargent DJ, Davis TM, Tobutt KR, Wilkinson MJ, Battey NH, Simpson DW. A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria. Theoretical and Applied Genetics. 2004 Nov; 109(7):1385-1391. |
| 2007 | Sargent DJ, Rys A, Nier S, Simpson DW, Tobutt KR. The development and mapping of functional markers in Fragaria and their transferability and potential for mapping in other genera. 2007 Jan; 114(2):373-384. |
Sequence
>FvNES1 ID=FvNES1; Name=FvNES1; organism=Fragaria vesca; type=genetic_marker; length=136bp
TGCTGATCATAGATCAGATGGCATCGTCTTCTCGGGCCTTCTTTAAAGTA
TTCAATCCTCAAATTGCCTGCTACTTTTGAGATAGCTTGCTTCCCAGTTC
TATTACTATAAAGCCGATGAACGTTGAAACCAAGCA
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