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Marker Overview
Name | PacA33 |
Genbank ID | N/A |
Type | SSR |
Species | Prunus armeniaca |
Germplasm | Stark Early Orange |
Source Type | cDNA |
Repeat Motif | (GA)16 |
PCR Condition | 2 mM of MgCl2, 0.1 mM of each dNTP, 0.2 mcM of each primer, 1? Buffer and 0.5 units of Taq polymerase (Sigma)in 25 mcl final volume. Thermocycling conditions were 30 s at 94 C, followed by 30 cycles of (94 ?C for 30 s, 57.5 C for 30 s and 72 C for 30 s) and an extension step at 72 C for 7 min. |
Primer 1 | PacA33.forward primer: TCAGTCTCATCCTGCATACG |
Primer 2 | PacA33.reverse primer: CATGTGGCTCAAGGATCAAA |
Product Length | 188/196 |
Polymorphism | P_ PacA33 |
Publication | [view all] |
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