UFFxa01E03 (genetic_marker) Fragaria ananassa

Marker Overview


Name	UFFxa01E03
Genbank ID	AJ870458
Type	SSR
Species	Fragaria x ananassa
Germplasm	Festival
Source Type	EST
Repeat Motif	(CAC)10 Direct
PCR Condition	Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min)
Primer 1	UFFxa01E03.Forward: ACCCCATCTTCTTCAAATCTCA
Primer 2	UFFxa01E03.Reverse (no gttt): GACAAGGCCAGAGCTAGAGAAG
Primer 3	UFFxa01E03.UFFxa01E03F: ACCCCATCTTCTTCAAATCTCA
Primer 4	UFFxa01E03.UFFxa01E03R: GACAAGGCCAGAGCTAGAGAAG
Publication	[view all]
Contact	D. Sargent Eric van de Weg

Contact

Name

Details

D. Sargent

First name:	Dan
Last name:	Sargent
Institution:	East Malling Research
Address:	New Road, East Malling, Kent ME19 6BJ, UK
Email:	dan.sargent@emr.ac.uk
Phone:	+44 1732 523747

Eric van de Weg

First name:	Eric
Last name:	van de Weg
Title:	researcher
Institution:	Wageningen-UR Plant Breeding, Wageningen University and Research Centre
Address:	P.O. Box 16, 6700 AA Wageningen, The Netherlands
Country:	The Netherlands
Email:	eric.vandeweg@wur.nl
Keywords:	allo-octoploid strawberry

Publications


Year	Publication
2006	Nier S, Simpson DW, Tobutt KR, Sargent DJ. A genetic linkage map of an inter-specific diploid Fragaria BC1 mapping population and its comparison with the Fragaria reference map (FV x FN). Journal of Horticultural Science and Biotechnology. 2006; 81(4):645-650.
2006	Sargent DJ, Clarke J, Simpson DW, Tobutt KR, Arús P, Monfort A, Vilanova S, Denoyes-Rothan B, Rousseau M, Folta KM, Bassil NV, Battey NH. An enhanced microsatellite map of diploid Fragaria. Theoretical and Applied Genetics. 2006 May; 112(7):1349-1359.
2006	Bassil N, Gunn M, Folta K, Lewers K. Microsatellite markers for Fragaria from 'Strawberry Festival' expressed sequence tags. Molecular ecology notes. 2006; 6(2):473-476.

Germplasm


Stock Name	Type
Festival	accession

Map Positions


#	Map Name	Linkage Group	Bin	Position	Locus	MapViewer
1	Fragaria-815x903-BC1	VI	N/A	19.56	UFFxa01E03	View
2	Fragaria-FVxFN-F2-2010	FG6	N/A	85.61	UFFxa01E03	View
3	Strawberry-HK-F1	LG6A	N/A	83.5	UFFxa01E03	View
4	Strawberry-HK-F1	LG6B	N/A	94	UFFxa01E03	View
5	Strawberry-HK-F1	LG6C	N/A	83.3	UFFxa01E03	View
6	Strawberry-HK-F1	LG6D	N/A	78	UFFxa01E03	View
7	Strawberry-NF-F2-2017	Fvb6	N/A	144.4	UFFxA01E03-HEX	View

Sequence

>UFFxa01E03 ID=UFFxa01E03; Name=UFFxa01E03; organism=Fragaria x ananassa; type=genetic_marker; length=185bp
ACCCCATCTTCTTCAAATCTCAAAACCCACAAACGGTGGTCTGTGTTCCC
CTACTGCCCTCCATGTCTCAACAAGCTTCTCCTCACCACCACCACCACCA
CCACCACCACCACCAAGAACAATCCATTCTCGCTTGCTCTCGACTCGTTG
CTCATTCTCTGCACTTCTCTAGCTCTGGCCTTGTC