| Library Name | Organism | Cultivar | Development Stage | Tissue Type | Number of ESTs | Note |
| LIBEST_015606 Prunus persica fruit mesocarp final maturation steps, post-climateric | Prunus persica | final maturation steps, post-climateric | fruit mesocarp | 23 | tissue_lib: S4 ; country: Italy | |
| LIBEST_027714 Pr_av_leaf_bud | Prunus avium | swollen bud | leaf bud | 6014 | E.coli NEB 10-beta | |
| LIBEST_016673 Prunus persica fruit mesocarp plus epidermis 80 days after bloom | Prunus persica | Fantasia | 80 days after bloom | fruit mesocarp plus epidermis | 928 | |
| LIBEST_012965 Ripe Apricot Fruit Lambda Zap II Library | Prunus armeniaca | Bergeron | Ripe stage | 4166 | Organ: Fruit; Vector: Lambda Zap II; Site_1: Eco RI; Site_2: XhoI; Oriented library, construction described in Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca cv. Bergeron) by Mbeguie-Mbeguie D, Chahine H, Gomez RM, Gouble B, Audergon JM, Souty M, Albagnac G, Fils-Lycaon B in Physiol Plant 105:294-303 1999 | |
| LIBEST_021001 Subtraction suppressive hybridization library A Forward | Prunus persica | Canadian Harmony on Tennessee Natural rootstock | current years growth | bark (mostly cambium) | 12 | Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Site_2: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the A Forward library represent sequences downregulated at 5oC in nightbreak. |
| LIBEST_026267 Fruits and flowers of Prunus mume cv. Xiyeqing | Prunus mume | Xiyeqing | mixed | Fruits and flowers | 4473 | Organ: Fruits and flowers; Vector: pDNR-LIB; Fruits and flowers of Prunus mume Xiyeqing at different development stages were collected from the resource nursery of Nanjing Agricultural University, Najing PRC. The materials were collected from 2009.2-2009.5. The flowers included four growing stages: small bud, medium bud, half-unfolding flower and completely unfolding flower, while the fruits were collected at 0.3, 0.6, 0.9, 1.2, 1.8, 2.4, 3.6 cm diameter stages. |
| LIBEST_019256 PU3 | Prunus persica | O`Henry | Mesocarp | 6639 | Organ: Fruit; Vector: pDNR-1r; Site_1: XhoI; Site_2: SmaI | |
| LIBEST_017722 Prunus armeniaca cv. Goldrich | Prunus armeniaca | Goldrich | 24 | |||
| LIBEST_020208 YG/PR | Prunus cerasus | Montmorency | Young-green and pre-ripe | Pericarp | 1243 | Organ: Fruit |
| LIBEST_009616 Cherry cDNA Library | Prunus avium | 21 | ||||
| LIBEST_011149 Peach developing fruit mesocarp | Prunus persica | Loring | Mesocarp | 9984 | Vector: pBluescript II SK(-); Site_1: EcoRI; Site_2: XhoI; authority=Prunus persica L. Batsh; The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis go to http://www.genome.clemson.edu/projects/peach. To order this clone go to http://www.genome.clemson.edu/orders | |
| LIBEST_012964 Green Apricot Fruit Lambda Zap II Library | Prunus armeniaca | Bergeron | Green stage | 5380 | Organ: Fruit; Vector: Lambda Zap II; Site_1: Eco RI; Site_2: XhoI; Oriented library, construction described in Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca cv. Bergeron) by Mbeguie-Mbeguie D, Chahine H, Gomez RM, Gouble B, Audergon JM, Souty M, Albagnac G, Fils-Lycaon B in Physiol Plant 105:294-303 1999 | |
| LIBEST_016335 S3 | Prunus persica | from pre-climacteric to climacteric | fruit mesocarp | 2919 | ||
| LIBEST_022200 TDFs from plum cv. Cacanska Najbolja | Prunus domestica | Cacanska Najbolja | leaf | 30 | Vector: pGEM-T; cDNA-AFLP was performed to identyfy genes differentially expressed in plum upon Plum pox virus (PPV) infection | |
| LIBEST_022919 Subtraction suppressive hybridization library C Reverse | Prunus persica | Canadian Harmony on Tennessee Natural rootstock | current years growth | bark (mostly cambium) | 23 | Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMCRT system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the C Reverse library represent sequences downregulated during night break photoperiods at 5oC. Night break simulates a long day (16 h light/8 h dark), but the actual light exposure is equivalent to a short day (8 h light/16 h dark) photoperiod. |
| LIBEST_018884 PU1 | Prunus persica | O`Henry | Mesocarp | 9729 | Organ: Fruit; Vector: pDNR-1r; Site_1: XhoI; Site_2: SmaI | |
| LIBEST_017153 Prunus persica L. leaf tissue, Plum Pox Virus infected | Prunus persica | Baby gold 5 | Leaf | 2144 | Leaf tissue of Prunus persica L. infected by Plum pox virus | |
| LIBEST_019257 PU4 | Prunus persica | O`Henry | Mesocarp | 6634 | Organ: Fruit; Vector: pDNR-1r; Site_1: XhoI; Site_2: SmaI | |
| LIBEST_022201 TDFs from plum cv. Cacanska Rodna | Prunus domestica | Cacanska Rodna | leaf | 24 | Vector: pGEM-T; cDNA-AFLP was performed to identyfy genes differentially expressed in plum upon Plum pox virus (PPV) infection | |
| LIBEST_027858 Peach fruit after harvest Lib | Prunus persica | Dixiland | mesocarp | 126 | Organ: fruit | |
| LIBEST_012963 Half-Ripe Apricot Fruit Lambda Zap II Library | Prunus armeniaca | Bergeron | Half-Ripe stage | 5522 | Organ: Fruit; Vector: Lambda Zap II; Site_1: Eco RI; Site_2: XhoI; Oriented library, construction described in Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca cv. Bergeron) by Mbeguie-Mbeguie D, Chahine H, Gomez RM, Gouble B, Audergon JM, Souty M, Albagnac G, Fils-Lycaon B in Physiol Plant 105:294-303 1999 | |
| LIBEST_016334 S4 | Prunus persica | post-climacteric | fruit mesocarp | 2908 | ||
| LIBEST_009614 Almond Lambda Zap II cDNA Library | Prunus dulcis | 64 | synonym: Prunus amygdalus | |||
| LIBEST_008685 sour cherry fruit library | Prunus cerasus | Montmorency | 8 days or 44 days after pollination | 12 | Organ: Fruit; Vector: pBluescriptSK; Site_1: 5-EcoRI; Site_2: 3-XhoI; PRA and PRB libraries were constructed with mRNA extracted from fruits harvested 44 days after pollination. YGC and YGD libraries were constructed from mRNA extracted from fruits 8 days after pollination. TAG_LIB=YGD | |
| LIBEST_010977 almond cDNA library | Prunus dulcis | pistils | 1006 | Organ: flower; Vector: pZL1; Site_1: Sal I; Site_2: Not I; Total RNAs were isolated from pistils using Trizol reagent (Invitrogen, USA). Then, polyA+ mRNA was isolated using oligo(dT) cellulose as described. cDNA was synthesized using a lambda-zipLox cDNA synthesis kit(CAT No.19643-014, Invitrogen, USA). The phage library was converted through mass excision to a plasmid library in the vector pZL1. The plasmid library was plated on 15-cm LB agar plates with 100ug/mL ampicillin. Individual clones were picked at random and propagated. The 5ends of the cDNA clones were sequenced on ABI Prism377 DNA sequencer. | ||
| LIBEST_010509 Apricot cDNA library | Prunus armeniaca | Stark Early Orange | Shoot bursting, post-dormancy | 11 | Organ: leaf; Vector: pGEM-T | |
| LIBEST_024792 RD | Prunus persica | adult | flower bud | 233 | Vector: pGEM-T Easy; Enriched in dormancy released bud | |
| LIBEST_018175 Cherry differentially expressed | Prunus avium x P. cerasus x P. canescens | Bing/Gisela5 or Bing/Gisela6 | Xylem, Bark | 89 | Organ: Stem; ve samples were excised from the cDNA-AFLP gel and amplified by PCR. The PCR product was cloned in the pGEM-T Easy vector and sequenced with the M13 forward primer. | |
| LIBEST_024303 Subtraction library (endodormant buds minus ecodormant buds) | Prunus mume | Nanko | lateral buds | 6 | country: Japan ; lat_lon: 34 N 135 E | |
| LIBEST_015035 S3II | Prunus persica | Redhaven | S3II, 95 days after full bloom | mesocarp | 1267 | |
| LIBEST_024793 ZS | Prunus persica | adult | flower bud | 62 | Vector: pGEM-T Easy; Enriched in Zincal 5 dormancy released bud | |
| LIBEST_024302 Prunus mume lateral buds | Prunus mume | Nanko | lateral buds | 1 | country: Japan ; lat_lon: 34 N 135 E | |
| LIBEST_022917 Subtraction suppressive hybridization library B Reverse | Prunus persica | Canadian Harmony on Tennessee Natural rootstock | current years growth | bark (mostly cambium) | 42 | Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the B Reverse library represent sequences downregulated during long day (16 h light/8 h dark) photoperiods at 25oC. |
| LIBEST_010486 Prunus persica fruit | Prunus persica | fruit | 4 | |||
| LIBEST_016672 Prunus persica fruit mesocarp plus epidermis 30 days after bloom | Prunus persica | Fantasia | 30 days after bloom | fruit mesocarp plus epidermis | 1647 | |
| LIBEST_024791 DR | Prunus persica | adult | flower bud | 96 | Vector: pGEM-T Easy; Enriched in dormant bud | |
| LIBEST_022411 PP1 | Prunus persica | Pop-DG | adult trees | mesocarp | 720 | Organ: fruits; Full-length cDNA library made from a mix of poly-A+ RNA from mesocarp of peach fruits stored at 5 degrees for different times, and showing symptoms of chilling injury. |
| LIBEST_022916 Subtraction suppressive hybridization library B Forward | Prunus persica | Canadian Harmony on Tennessee Natural rootstock | current years growth | bark (mostly cambium) | 46 | Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Site_2: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the B Forward library represent sequences upregulated during long day photoperiods (16 h light/8 h dark) at 25oC. |
| LIBEST_027181 Suppression Subtractive Hybridization Library of Floral Buds in Prunus persica during Domancy-Releasing | Prunus persica | floral Bud | 9 | |||
| LIBEST_016802 S2 | Prunus persica | endocarp hardening | fruit mesocarp | 156 | ||
| LIBEST_022920 Subtraction suppressive hybridization library E Forward | Prunus persica | Canadian Harmony on Tennessee Natural rootstock | current years growth | bark (mostly cambium) | 16 | Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the E Forward library represent sequences upregulated at 5oC during exposure to short day (8 h light/16 h dark) photoperiods. |
| LIBEST_007302 near-ripe peach fruit cDNA library | Prunus persica | Loring | 21-60 Newtons flesh resistance | Whole fruit minus the stone | 174 | Vector: Lambda vector UniZap XR; Site_1: EcoRI; Site_2: XhoI; Fruit cDNA. The mRNA was isolated from whole fruit from which the stone was excised. The fruit was near ripe, registering from 21 to 60 newtons resistance to a penetrometer. The library was constructed using the lambda UniZap XR by Stratagene. |
| LIBEST_015605 Prunus persica fruit mesocarp final maturation steps, pre-climateric | Prunus persica | final maturation steps, pre-climateric | fruit mesocarp | 12 | tissue_lib: S3 ; country: Italy | |
| LIBEST_017154 Prunus persica L. leaf tissue, healthy | Prunus persica | Baby gold 5 | Leaf | 1625 | Leaf tissue of Prunus persica L. not infected by Plum pox virus | |
| LIBEST_018856 Peach shoot | Prunus persica | Loring | Shoot | 7085 | Vector: pBluescript II SK(-); Site_1: EcoRI; Site_2: XhoI | |
| LIBEST_025548 Floral organ cDNA library of Mei flower | Prunus mume | 92 | Vector: lTripIEx2 | |||
| LIBEST_022918 Subtraction suppressive hybridization library C Forward | Prunus persica | Canadian Harmony on Tennessee Natural rootstock | current years growth | bark (mostly cambium) | 22 | Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the C Forward library represent sequences upregulated during night break photoperiods at 5oC. Night break simulates a long day (16 h light/8 h dark) photoperiod, but the actual light exposure is equivalent to a short day (8 h light/16 h dark) exposure. |
| LIBEST_022412 PPN | Prunus persica | Pop-DG | adult trees | mesocarp | 7142 | Organ: fruits; Full-length, normalized cDNA library made from a mix of poly-A+ RNA from mesocarp of peach fruits stored at 5 degress for different times, and showing symptoms of chilling injury. |
| LIBEST_009893 Prunus persica green fruit | Prunus persica | Suncrest | green fruit | 1 | ||
| LIBEST_018885 PU2 | Prunus persica | O`Henry | Mesocarp | 9495 | Organ: Fruit; Vector: pDNR-1r; Site_1: XhoI; Site_2: SmaI | |
| LIBEST_015940 Apricot cv. Colomer cDNA library | Prunus armeniaca | Colomer | grafted scion | 2 | Organ: leaf; Vector: pGEM-T; mRNA extracted from leaf samples originating from apricot grafted scion | |
| LIBEST_011273 Almond developing seed | Prunus dulcis | Nonpareil | embryo | 2794 | Vector: pBK-CMV; Site_1: EcoRI; Site_2: XhoI; synonym: Prunus amygdalus | |
| LIBEST_017210 Peach developing fruit mesocarp Stage S4 | Prunus persica | Yumyeong | Mesocarp | 1667 | Vector: pDONR222 | |
| LIBEST_024005 Differentially expressed cDNAs between wild-type and evergrowing Prunus persica from long-days to short-days transition | Prunus persica | current years growth | apical bud tip | 180 | Vector: pGEM-T easy; Total RNA was isolated from apical bud tips from peach. After DNase I treatment (Invitrogen, Carlsbad, CA, USA), polyA+ RNA was purified using Dynabeads Oligo(dT)25 (Invitrogen). The cDNA was synthetised and the suppression subtractive hybridization (SSH) between WT and evg samples at LD and one, two, four and eight weeks of SD was performed following the manufacturers protocol (Clontech Laboratories, Palo Alto, CA, USA). PCR-amplified products were cloned into pGEM-T easy vector (Promega, Madison, WI, USA). 5,760 clones from subtracted cDNA libraries were screened by hybridization with forward- and reverse-subtracted radiolabeled cDNA. Selected clones from all time points and directions were sequenced using pGEM-T easy vector primer. | |
| LIBEST_016803 S1 | Prunus persica | post-fertilization | fruit mesocarp | 137 | ||
| LIBEST_024301 Subtraction library (endodormant buds minus paradormant buds) | Prunus mume | Nanko | lateral buds | 17 | country: Japan ; lat_lon: 34 N 135 E | |
| LIBEST_009615 Peach cDNA Library | Prunus persica | 22 | ||||
| LIBEST_026655 PLMVd-infected GF-305 peach tree leaves | Prunus persica | GF-305 | Leaves | 13 | Sequence obtained by cDNA-AFLP | |
| LIBEST_020449 Prunus persica fruit skin mature fruit | Prunus persica | Bolero | mature fruit | fruit skin | 4690 | country: Italy |
| LIBEST_024794 SZ | Prunus persica | adult | flower bud | 73 | Vector: pGEM-T Easy; Enriched in Springlady dormancy released bud | |
| LIBEST_021000 Subtraction suppressive hybridization library A Reverse | Prunus persica | Canadian Harmony on Tennessee Natural rootstock | current years growth | bark (mostly cambium) | 21 | Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the A Reverse library (AR library) represent sequences upregulated at 5oC in nightbreak. |
| LIBEST_015585 Prunus persica mesocarp S4 climacteric | Prunus persica | Fantasia | S4 climacteric | mesocarp | 1049 | |
| LIBEST_022921 Subtraction suppressive hybridization library E Reverse | Prunus persica | Canadian Harmony on Tennessee Natural rootstock | current years growth | bark (mostly cambium) | 40 | Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the E Reverse library represent sequences downregulated at 5oC during exposure to short day (8 h light/16 h dark) photoperiods. |
