QTL mapping for brown rot (Monilinia fructigena) resistance in an intraspecific peach (Prunus persica L. Batsch) F1 progeny

Publication Overview
TitleQTL mapping for brown rot (Monilinia fructigena) resistance in an intraspecific peach (Prunus persica L. Batsch) F1 progeny
AuthorsPacheco I, Bassi D, Eduardo I, Ciacciulli A, Pirona R, Rossini L, Vecchietti A
TypeJournal Article
Journal NameTree genetics & genomes
Volume10
Issue5
Year2014
Page(s)1223-1242
CitationPacheco I, Bassi D, Eduardo I, Ciacciulli A, Pirona R, Rossini L, Vecchietti A. QTL mapping for brown rot (Monilinia fructigena) resistance in an intraspecific peach (Prunus persica L. Batsch) F1 progeny. Tree genetics & genomes. 2014; 10(5):1223-1242.

Abstract

Brown rot (BR) caused by Monilinia spp. leads to significant post-harvest losses in stone fruit production, especially peach. Previous genetic analyses in peach progenies suggested that BR resistance segregates as a quantitative trait. In order to uncover genomic regions associated with this trait and identify molecular markers for assisted selection (MAS) in peach, an F1 progeny from the cross “Contender” (C, resistant) × “Elegant Lady” (EL, susceptible) was chosen for quantitative trait loci (QTL) analysis. Over two phenotyping seasons, skin (SK) and flesh (FL) artificial infections were performed on fruits using a Monilinia fructigena isolate. For each treatment, infection frequency (if) and average rot diameter (rd) were scored. Significant seasonal and intertrait correlations were found. Maturity date (MD) was significantly correlated with disease impact. Sixty-three simple sequence repeats (SSRs) plus 26 single-nucleotide polymorphism (SNP) markers were used to genotype the C × EL population and to construct a linkage map. C × EL map included the eight Prunus linkage groups (LG), spanning 572.92 cM, with an average interval distance of 6.9 cM, covering 78.73 % of the peach genome (V1.0). Multiple QTL mapping analysis including MD trait as covariate uncovered three genomic regions associated with BR resistance in the two phenotyping seasons: one containing QTLs for SK resistance traits near M1a (LG C × EL-2, R ²²âââ¹= 13.1–31.5 %) and EPPISF032 (LG C × EL-4, R ²²âââ¹= 11–14 %) and the others containing QTLs for FL resistance, near markers SNP_IGA_320761 and SNP_IGA_321601 (LG3, R ²²âââ¹= 3.0–11.0 %). These results suggest that in the C × EL F1 progeny, skin resistance to fungal penetration and flesh resistance to rot spread are distinguishable mechanisms constituting BR resistance trait, associated with different genomic regions. Discovered QTLs and their associated markers could assist selection of new cultivars with enhanced resistance to Monilinia spp. in fruit.
Projects
This publication contains information about 1 projects:
Project NameDescription
Prunus-resistance to Monilinia fructicola-Pacheco-2014
Features
This publication contains information about 40 features:
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snp460591snp460591genetic_marker
UPD96-013UPD96-013genetic_marker
ssrPACITA06ssrPACITA06genetic_marker
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snp389048snp389048genetic_marker
snp401886snp401886genetic_marker
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snp532100snp532100genetic_marker
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resistance to Monilinia fructicolaqRMF.CxEL-LG2.2010.Sk-ifQTL
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resistance to Monilinia fructicolaqRMF.CxEL-LG4.2010.Sk-if.2QTL
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resistance to Monilinia fructicolaqRMF.CxEL-LG4.2009.Fl-rd.2QTL

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Peach_CxEL_F1
Peach_CxEL_F1-physical-Prunus-persicaV1.0
Properties
Additional details for this publication include:
Property NameValue
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s11295-014-0756-7
Publication TypeJournal Article
Publication Date2014
Published Location|||
Language Abbreng
KeywordsMonilinia fructigena, Prunus persica, chromosome mapping, cultivars, disease resistance, fruits, fungi, genetic markers, genome, genotype, linkage groups, microsatellite repeats, peaches, phenotype, postharvest losses, progeny, quantitative trait loci, single nucleotide polymorphism
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4937083