Malus x domestica Whole Genome v3.0.a1 Assembly & Annotation
The Apple genome assembly version 3.0 mainly involved anchoring of contigs using a manually curated consensus genetic map obtained from from 21 genetic maps and a new structure of scaffolds. Minor modifications were also made to the set of contigs,reducing them to 94,174 .
New scaffolds were made by exploiting 30X coverage of SOLiD mate pair reads with a single insert size of 5 Kb. Scaffolds were created by mapping reads against contigs in single end mode with Bfast aligner (http://sourceforge.net/projects/bfast/) and physical relationships were then reconstructed and filtered by means of in-house developed software.
Contigs and scaffolds were then anchored to chromosomes (LG) by using a consensus of 21 genetic maps (using about 16,000 genetic markers obtained from the Apple illumina 20K SNP chip array, Bianco et al. 2014 PLoS ONE 9(10): e110377). This provided information for 66,124 contigs (589,070 Kbp).
Asssembly 3.0 is provided as gff3 by the Riccardo Velasco group with contigs coordinates on LG and (to grant back compatibility with previous version) also as primary and 3 alternative assemblies (see description of Malus x domestica genome v2.0 for details).
Note: Gene alignments to contigs can be viewed in GBrowse Malus x domestica v3.0.a1 contigs and contig alignments to LG can be viewed in GBrowse Malus x domestica v3.0.a1 pseudomolecules.
All assembly and annotation files are available for download by selecting the desired data type in the right-hand side bar. Each data type page will provide a description of the available files and links to download.
The Malus x domestica v3.0.a1 genome assembly files are available in FASTA and GFF3 formats. There are a total of 17 pseudomolecules in this assembly of apple.
Primary and three alternative assemblies
Repeats were predicted and provided as tab-delimited and fasta files below.
The Malus x domestica v1.0 genes are aligned to v3.0.a1 genome and provided as download below: