High-density mapping suggests cytoplasmic male sterility with two restorer genes in almond × peach progenies

Publication Overview
TitleHigh-density mapping suggests cytoplasmic male sterility with two restorer genes in almond × peach progenies
AuthorsDonoso JM, Eduardo I, Picañol R, Batlle I, Howad W, Aranzana MJ, Arús P.
TypeJournal Article
Journal NameHorticulture Research
Volume2
Year2015
CitationDonoso JM, Eduardo I, Picañol R, Batlle I, Howad W, Aranzana MJ, Arús P. High-density mapping suggests cytoplasmic male sterility with two restorer genes in almond × peach progenies. Horticulture Research 2015 2: 15016

Abstract

Peach (Prunus persica) and almond (Prunus dulcis) are two sexually compatible species that produce fertile offspring. Almond, a highly polymorphic species, is a potential source of new genes for peach that has a strongly eroded gene pool. Here we describe the genetics of a male sterile phenotype that segregated in two almond (‘Texas’) × peach (‘Earlygold’) progenies: an F2 (T×E) and a backcross one (T1E) to the ‘Earlygold’ parent. High-density maps were developed using a 9k peach SNP chip and 135 simple-sequence repeats. Three highly syntenic and collinear maps were obtained: one for the F2 (T×E) and two for the backcross, T1E (for the hybrid) and E (for ‘Earlygold’). A major reduction of recombination was observed in the interspecific maps (T×E and T1E) compared to the intraspecific parent (E). The E map also had extensive monomorphic genomic regions suggesting the presence of large DNA fragments identical by descent. Our data for the male sterility character were consistent with the existence of cytoplasmic male sterility, where individuals having the almond cytoplasm required the almond allele in at least one of two independent restorer genes, Rf1 and Rf2, to be fertile. The restorer genes were located in a 3.4 Mbp fragment of linkage group 2 (Rf1) and 1.4 Mbp of linkage group 6 (Rf2). Both fragments contained several genes coding for pentatricopeptide proteins, demonstrated to be responsible for restoring fertility in other species. The implications of these results for using almond as a source of novel variability in peach are discussed.
File Attachments
AttachmentSize
File TxE_GDR.xlsx868.74 KB
File T1E_GDR.xlsx2.71 MB
File E_GDR.xlsx816.01 KB
File MS_GDR.xlsx1.47 MB
Features
This publication contains information about 480 features:
Feature NameUniquenameType
SNP_IGA_412662SNP_IGA_412662genetic_marker
SNP_IGA_413115SNP_IGA_413115genetic_marker
SNP_IGA_415301SNP_IGA_415301genetic_marker
SNP_IGA_418582SNP_IGA_418582genetic_marker
SNP_IGA_419762SNP_IGA_419762genetic_marker
SNP_IGA_420316SNP_IGA_420316genetic_marker
SNP_IGA_425622SNP_IGA_425622genetic_marker
SNP_IGA_428047SNP_IGA_428047genetic_marker
SNP_IGA_428056SNP_IGA_428056genetic_marker
SNP_IGA_431437SNP_IGA_431437genetic_marker
SNP_IGA_433050SNP_IGA_433050genetic_marker
SNP_IGA_440662SNP_IGA_440662genetic_marker
SNP_IGA_456188SNP_IGA_456188genetic_marker
SNP_IGA_482102SNP_IGA_482102genetic_marker
SNP_IGA_5258SNP_IGA_5258genetic_marker
SNP_IGA_550504SNP_IGA_550504genetic_marker
SNP_IGA_55903SNP_IGA_55903genetic_marker
SNP_IGA_562867SNP_IGA_562867genetic_marker
SNP_IGA_5726SNP_IGA_5726genetic_marker
SNP_IGA_575030SNP_IGA_575030genetic_marker
SNP_IGA_582600SNP_IGA_582600genetic_marker
SNP_IGA_584315SNP_IGA_584315genetic_marker
SNP_IGA_586202SNP_IGA_586202genetic_marker
SNP_IGA_58626SNP_IGA_58626genetic_marker
SNP_IGA_587450SNP_IGA_587450genetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Prunus-TE-F2-2015
Prunus-TE-BC1-T1E
Prunus-TE-BC1-E
Properties
Additional details for this publication include:
Property NameValue
URLhttp://www.nature.com/articles/hortres201516