Publication Overview
TitleMicrosatellite marker development in rose and its application in tetraploid mapping
AuthorsZhang L.H., Byrne D.H., Ballard R.E., Rajapakse S.
TypeJournal Article
Journal NameJournal of the American Society for Horticultural Science
Volume131
Issue3
Year2006
Page(s)380-387
CitationZhang L.H., Byrne D.H., Ballard R.E., Rajapakse S.. Microsatellite marker development in rose and its application in tetraploid mapping. Journal of the American Society for Horticultural Science. 2006; 131(3):380-387.

Abstract

Microsatellite or simple sequence repeat (SSR) markers were developed from Rosa wichurana Crépin to combine two previously constructed tetraploid rose (Rosa hybrida L.) genetic maps. To isolate SSR-containing sequences from rose a small-insert genomic library was constructed from diploid Rosa wichurana and screened with several SSR probes. Specific primers were designed for 43 unique SSR regions, of which 30 primer pairs gave rise to clear PCR products. Seventeen SSR primer pairs (57%) produced polymorphism in the tetraploid rose 90-69 mapping family. These markers were incorporated into existing maps of the parents 86-7 and 82-1134, which were constructed primarily with AFLP markers. The current map of the male parent, amphidiploid 86-7, consists of 286 markers assigned to 14 linkage groups and covering 770 cM. The map of the female tetraploid parent, 82-1134, consists of 256 markers assigned to 20 linkage groups and covering 920 cM. Nineteen rose SSR loci were mapped on the 86-7 map and 11 on the 82-1134 map. Several homeologous linkage groups within maps were identified based on SSR markers. In addition, some of the SSR markers provided anchoring points between the two parental maps. SSR markers were also useful for joining small linkage groups. Based on shared SSR markers, consensus orders for four rose linkage groups between parental maps were generated. Microsatellite markers developed in this study will provide valuable tools for many aspects of rose research including future consolidation of diploid and tetraploid rose genetic linkage maps, genetic, phylogenetic and population analyses, cultivar identification, and marker-assisted selection.
Features
This publication contains information about 531 features:
Feature NameUniquenameType
RW8B8RW8B8genetic_marker
RW14A5RW14A5genetic_marker
Rw1F9Rw1F9genetic_marker
Rw3N19Rw3N19genetic_marker
RW11E5RW11E5genetic_marker
RW14H21RW14H21genetic_marker
Rw17I17Rw17I17genetic_marker
RW18N19RW18N19genetic_marker
Rw23H5Rw23H5genetic_marker
Rw27A11Rw27A11genetic_marker
RW32D19RW32D19genetic_marker
Rw45E24Rw45E24genetic_marker
Rw61F2Rw61F2genetic_marker
Rw62C4Rw62C4genetic_marker
PGCMCCA23PGCMCCA23genetic_marker
PGCMCCA22PGCMCCA22genetic_marker
PGCMCCA21PGCMCCA21genetic_marker
PGCMCCA20PGCMCCA20genetic_marker
PGCMCCA19PGCMCCA19genetic_marker
PGCMCCA18PGCMCCA18genetic_marker
PGCMCCA16PGCMCCA16genetic_marker
PGCMCCA15PGCMCCA15genetic_marker
PGCMCCA14PGCMCCA14genetic_marker
PGCMCCA13PGCMCCA13genetic_marker
PGCMCCA12PGCMCCA12genetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Rose-82-1134-F2
Rose-86-7-F2
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
90-6990-69accession
82-113482-1134accession
86-786-7accession
90-69_F290-69_F2population