Publication Overview
TitleConstruction of a High-Density Simple Sequence Repeat Consensus Genetic Map for Pear (Pyrus spp.)
AuthorsChen H, Song Y, Li L, Khan M, Korban S, Wu J, Zhang S
TypeJournal Article
Journal NamePlant Molecular Biology Reporter
Volume33
Issue2
Year2015
Page(s)316–325
CitationChen H, Song Y, Li L, Khan M, Korban S, Wu J, Zhang S. Construction of a High-Density Simple Sequence Repeat Consensus Genetic Map for Pear (Pyrus spp.). Plant Molecular Biology Reporter. 2015; 33(2):316–325.

Abstract

As an important fruit crop that is widely grown commercially in temperate regions of the world, pear (Pyrus) is a target for pursuing efficient breeding strategies. Construction of a reliable and dense genetic linkage map is critical for undertaking marker-assisted breeding. In this study, a population of 56 F1 seedlings of ‘Bayuehong’ × ‘Dangshansuli’ was used to construct a high-density simple sequence repeat (SSR)-based genetic linkage map. A total of 1,756 SSR markers, including 1,341 newly designed SSRs based on whole-genome sequencing of an Asiatic pear along with 415 previously reported SSRs, were first evaluated for polymorphism. Based on 894 SSRs demonstrating polymorphism, a consensus genetic map consisting of 734 loci distributed along all 17 linkage groups (LG) was constructed, with a total length of 1,661.4 cM and with an average marker interval of 2.26 cM. Comparisons among different maps of pear and apple were then made based on positions of previously mapped SSR markers on the consensus map. As a result, homologous linkage groups LG3 and LG11, LG5 and LG10, LG9 and LG17, LG13 and LG16, LG8 and LG15 have been identified. This high-density SSR map along with a set of SSR markers covering the whole genome of pear will greatly facilitate integration of independent maps, aid in pursuing comparative genome studies, and in evaluation of different germplasm in future genetic and breeding studies.
Features
This publication contains information about 437 features:
Feature NameUniquenameType
MES136MES136genetic_marker
MES138MES138genetic_marker
MES16MES16genetic_marker
NAUpy01hNAUpy01hgenetic_marker
NAUpy01nNAUpy01ngenetic_marker
NAUpy01tNAUpy01tgenetic_marker
NAUpy02aNAUpy02agenetic_marker
NAUpy02bNAUpy02bgenetic_marker
NAUpy02eNAUpy02egenetic_marker
NAUpy02kNAUpy02kgenetic_marker
NAUpy03dNAUpy03dgenetic_marker
NAUpy03eNAUpy03egenetic_marker
NAUpy04cNAUpy04cgenetic_marker
NAUpy04dNAUpy04dgenetic_marker
NAUpy04eNAUpy04egenetic_marker
NAUpy04hNAUpy04hgenetic_marker
NAUpy04vNAUpy04vgenetic_marker
NAUpy05aNAUpy05agenetic_marker
NAUpy05eNAUpy05egenetic_marker
NAUpy05rNAUpy05rgenetic_marker
NAUpy05zNAUpy05zgenetic_marker
NAUpy06hNAUpy06hgenetic_marker
NAUpy06wNAUpy06wgenetic_marker
NAUpy07eNAUpy07egenetic_marker
NAUpy07hNAUpy07hgenetic_marker

Pages

Featuremaps
This publication contains information about 5 maps:
Map Name
Pear-BD-F1-2015
Pear-Bayuehong-F1-2015
Pear-Dangshansuli-F1-2015
Pear-Bartlett-F1-2007
Pear-La_France-F1-2007
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
Bayuehong_x_DangshansuliBayuehong_x_Dangshansulipopulation
DangshansuliDangshansulicultivar
BayuehongBayuehongcultivar