Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region

Publication Overview
TitleNarrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region
AuthorsRuiz EMV, Soriano JM, Romero C, Zhebentyayeva T, Terol J, Zuriaga E, Llácer G, Abbott AG, Badenes ML
TypeJournal Article
Journal NameMolecular Plant Pathology
Volume12
Issue6
Year2011
Page(s)535–547
CitationRuiz EMV, Soriano JM, Romero C, Zhebentyayeva T, Terol J, Zuriaga E, Llácer G, Abbott AG, Badenes ML. Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region. Molecular Plant Pathology. 2011; 12(6):535–547.

Abstract

Sharka disease, caused by the Plum pox virus (PPV), is one of the main limiting factors for stone fruit crops worldwide. Only a few resistance sources have been found in apricot (Prunus armeniaca L.), and most studies have located a major PPV resistance locus (PPVres) on linkage group 1 (LG1). However, the mapping accuracy was not sufficiently reliable and PPVres was predicted within a low confidence interval. In this study, we have constructed two high-density simple sequence repeat (SSR) improved maps with 0.70 and 0.68 markers/cm, corresponding to LG1 of ‘Lito’ and ‘Goldrich’ PPV-resistant cultivars, respectively. Using these maps, and excluding genotype– phenotype incongruent individuals, a new binary trait locus (BTL) analysis for PPV resistance was performed, narrowing down the PPVres support intervals to 7.3 and 5.9 cm in ‘Lito’ and ‘Goldrich’, respectively. Subsequently, 71 overlapping oligonucleotides (overgo) probes were hybridized against an apricot bacterial artificial chromosome (BAC) library, identifying 870 single BACs from which 340 were anchored onto a map region of approximately 30–40 cm encompassing PPVres. Partial BAC contigs assigned to the two allelic haplotypes (resistant/susceptible) of the PPVres locus were built by highinformation content fingerprinting (HICF). In addition, a total of 300 BAC-derived sequences were obtained, and 257 showed significant homology with the peach genome scaffold_1 corresponding to LG1. According to the peach syntenic genome sequence, PPVres was predicted within a region of 2.16 Mb in which a few candidate resistance genes were identified
Projects
This publication contains information about 1 projects:
Project NameDescription
Apricot PPV resistance trait-Badenes-2011
Featuremaps
This publication contains information about 4 maps:
Map Name
Apricot-GC-F1
Apricot-LL-F2
Apricot-LL-F2-Lito-2011
Apricot-integrated-Goldrich-2011