Prunus zhengheensis Whole v1.0 Genome Assembly & Annotation

Overview
Analysis NamePrunus zhengheensis Whole v1.0 Genome Assembly & Annotation
MethodHifiasm (v0.16.1)
SourceIllumina HiSeq and PacBio reads
Date performed2024-10-16

Publication

Haplotype-resolved genome of Prunus zhengheensis provides insight into its evolution and low temperature adaptation in apricot 
Wei Tan, Pengyu Zhou, Xiao Huang, Ruyu Liao, Xiaoan Wang, Yaoyao Wu, Zhaojun Ni, Ting Shi, Xiaqing Yu, Huiqin Zhang, Chengdong Ma, Feng Gao, Yufan Ma, Yang Bai, Faisal Hayat, Ouma Kenneth Omondi, Daouda Coulibaly, Zhihong Gao
Horticulture Research, Volume 11, Issue 4, April 2024, uhae103, https://doi.org/10.1093/hr/uhae103

Abstract

Prunus zhengheensis, an extremely rare population of apricots, originated in warm South-East China and is an excellent material for genetic breeding. However, most apricots and two related species (P. sibirica, P. mandshurica) are found in the cold northern regions in China and the mechanism of their distribution is still unclear. In addition, the classification status of P. zhengheensis is controversial. Thus, we generated a high-quality haplotype-resolved genome for P. zhengheensis, exploring key genetic variations in its adaptation and the causes of phylogenetic incongruence. We found extensive phylogenetic discordances between the nuclear and organelle phylogenies of P. zhengheensis, which could be explained by incomplete lineage sorting. A 242.22-Mb pan-genome of the Armeniaca section was developed with 13 chromosomal genomes. Importantly, we identified a 566-bp insertion in the promoter of the HSFA1d gene in apricot and showed that the activity of the HSFA1d promoter increased under low temperatures. In addition, HSFA1d overexpression in Arabidopsis thaliana indicated that HSFA1d positively regulated plant growth under chilling. Therefore, we hypothesized that the insertion in the promoter of HSFA1d in apricot improved its low-temperature adaptation, allowing it to thrive in relatively cold locations. 
The findings help explain the weather adaptability of Armeniaca plants.

 

Table 1  Genome assembly statistics for Prunus zhengheensis.
  hap1 hap2
Total length (bp) 241 369 245 235 573 950
Total number 46 39
Scaffold N50 (bp) 21 242 234 16 520 266
Contig N90 (bp) 9 432 709 5 619 964
Average (bp) 5 247 157.50 6 040 357.69
Maximum (bp) 30 595 378 28 518 252
GC% 37.83 37.77
Repeat content (%) 45.40 44.31
Protein-coding genes 25 986 26 295
BUSCO completeness (%) 93.6 92.6
Complete and single-copy BUSCOs (%) 91.6 91.3
Homology

Homology of the Prunus zhengheensis genome v1.0 proteins was determined by pairwise sequence comparison using the blastp algorithm against various protein databases. An expectation value cutoff less than 1e-6  for the Arabidoposis proteins (Araport11, 2022-09), UniProtKB/SwissProt (Release 2024-03), and UniProtKB/TrEMBL (Release 2024-03) databases. The best hit reports are available for download in Excel format. 

Protein Homologs

P. zhengheensis v1.0 proteins with arabidopsis (Araport11) homologs (EXCEL file) Pzhengheensis_v1.0_vs_arabidopsis.xlsx.gz
P. zhengheensis v1.0 proteins with arabidopsis (Araport11) (FASTA file) Pzhengheensis_v1.0_vs_arabidopsis_hit.fasta.gz
P. zhengheensis v1.0 proteins without arabidopsis (Araport11) (FASTA file) Pzhengheensis_v1.0_vs_arabidopsis_noHit.fasta.gz
P. zhengheensis v1.0 proteins with SwissProt homologs (EXCEL file) Pzhengheensis_v1.0_vs_swissprot.xlsx.gz
P. zhengheensis v1.0 proteins with SwissProt (FASTA file) Pzhengheensis_v1.0_vs_swissprot_hit.fasta.gz
P. zhengheensis v1.0 proteins without SwissProt (FASTA file) Pzhengheensis_v1.0_vs_swissprot_noHit.fasta.gz
P. zhengheensis v1.0 proteins with TrEMBL homologs (EXCEL file) Pzhengheensis_v1.0_vs_trembl.xlsx.gz
P. zhengheensis v1.0 proteins with TrEMBL (FASTA file) Pzhengheensis_v1.0_vs_trembl_hit.fasta.gz
P. zhengheensis v1.0 proteins without TrEMBL (FASTA file) Pzhengheensis_v1.0_vs_trembl_noHit.fasta.gz
Assembly

The Prunus zhengheensis Genome v1.0 assembly files are available in FASTA format.

Downloads

Chromosomes (haplome A) (FASTA file) Prunus_zhengheensis_HAP1_v1.0.a1.fasta.gz
Chromosomes (haplome B) (FASTA file) Prunus_zhengheensis_HAP2_v1.0.a1.fasta.gz
Gene Predictions

The Prunus zhengheensis Genome v1.0 gene prediction file are available in GFF3 and FASTA format.

Downloads

Genes (haplome A) (GFF3 file) Prunus_zhengheensis_HAP1_v1.0.a1.genes.gff3.gz
Genes (haplome B) (GFF3 file) Prunus_zhengheensis_HAP2_v1.0.a1.genes.gff3.gz
Protein sequences (haplome A) (FASTA file) Prunus_zhengheensis_HAP1_v1.0.a1.protein.fa.gz
Protein sequences (haplome B) (FASTA file) Prunus_zhengheensis_HAP2_v1.0.a1.protein.fa.gz
CDS sequences (haplome A) (FASTA file) Prunus_zhengheensis_HAP1_v1.0.a1.cds.fa.gz
CDS sequences (haplome B) (FASTA file) Prunus_zhengheensis_HAP2_v1.0.a1.cds.fa.gz
Functional Analysis

Functional annotation for the Prunus zhengheensis genome v1.0 are available for download below. The P. zhengheensis genome v1.0 proteins were analyzed using InterProScan in order to assign InterPro domains and Gene Ontology (GO) terms. Pathways analysis was performed using the KEGG Automatic Annotation Server (KAAS).

Downloads

GO assignments from InterProScan Pzhengheensis_v1.0_genes2GO.xlsx.gz
IPR assignments from InterProScan Pzhengheensis_v1.0_genes2IPR.xlsx.gz
Proteins mapped to KEGG Orthologs Pzhengheensis_v1.0_KEGG-orthologis.xlsx.gz
Proteins mapped to KEGG Pathways Pzhengheensis_v1.0_KEGG-pathways.xlsx.gz
Transcript Alignments
Transcript alignments were performed by the GDR Team of Main Bioinformatics Lab at WSU. The alignment tool 'BLAT' was used to map transcripts to the Prunus zhengheensis genome assembly. Alignments with an alignment length of 97% and 97% identify were preserved. The available files are in GFF3.
Fragaria x ananassa GDR RefTrans v1 Pzhengheensis_v1.0_f.x.ananassa_GDR_reftransV1
P. armeniaca GDR RefTrans v1 Pzhengheensis_v1.0_p.avium_GDR_reftransV1
Prunus persica GDR RefTrans v1 Pzhengheensis_v1.0_p.persica_GDR_reftransV1
Rosa GDR RefTrans v1 Pzhengheensis_v1.0_rosa_GDR_reftransV1
Rubus GDR RefTrans v2 Pzhengheensis_v1.0_rubus_GDR_reftransV2
Malus_x_domestica GDR RefTrans v1 Pzhengheensis_v1.0_m.x.domestica_GDR_reftransV1
Pyrus GDR RefTrans v1 Pzhengheensis_v1.0_pyrus_GDR_reftransV1