High-density genetic linkage map construction and identification of fruit-related QTLs in pear using SNP and SSR markers

Publication Overview
TitleHigh-density genetic linkage map construction and identification of fruit-related QTLs in pear using SNP and SSR markers
AuthorsWu J, Li LT, Li M, Khan MA, Li XG, Chen H, Yin H, Zhang SL
TypeJournal Article
Journal NameJournal of experimental botany
Year2014
CitationWu J, Li LT, Li M, Khan MA, Li XG, Chen H, Yin H, Zhang SL. High-density genetic linkage map construction and identification of fruit-related QTLs in pear using SNP and SSR markers. Journal of experimental botany. 2014 Aug 16.

Abstract

Pear (Pyrus spp) is an important fruit crop, grown in all temperate regions of the world, with global production ranked after grape and apples among deciduous tree crops. A high-density linkage map is a valuable tool for fine mapping quantitative trait loci (QTL) and map-based gene cloning. In this study, we firstly constructed a high-density linkage map of pear using SNPs integrated with SSRs, developed by the rapid and robust technology of restriction-associated DNA sequencing (RADseq). The linkage map consists of 3143 SNP markers and 98 SSRs, 3241 markers in total, spanning 2243.4 cM, with an average marker distance of 0.70 cM. Anchoring SSRs were able to anchor seventeen linkage groups to their corresponding chromosomes. Based on this high-density integrated pear linkage map and two years of fruit phenotyping, a total of 32 potential QTLs for 11 traits, including length of pedicel (LFP), single fruit weight (SFW), soluble solid content (SSC), transverse diameter (TD), vertical diameter (VD), calyx status (CS), flesh colour (FC), juice content (JC), number of seeds (NS), skin colour (SC), and skin smooth (SS), were identified and positioned on the genetic map. Among them, some important fruit-related traits have for the first time been identified, such as calyx status, length of pedicel, and flesh colour, and reliable localization of QTLs were verified repeatable. This high-density linkage map of pear is a worthy reference for mapping important fruit traits, QTL identification, and comparison and combination of different genetic maps.

Projects
This publication contains information about 1 projects:
Project NameDescription
Pear-fruit_phenotype-Wu-2014
Features
This publication contains information about 3,175 features:
Feature NameUniquenameType
Pyb05_092Pyb05_092genetic_marker
Pyb05_101Pyb05_101genetic_marker
Pyb05_102Pyb05_102genetic_marker
Pyb05_103Pyb05_103genetic_marker
Pyb05_104Pyb05_104genetic_marker
Pyb05_105Pyb05_105genetic_marker
Pyb05_106Pyb05_106genetic_marker
Pyb05_110Pyb05_110genetic_marker
Pyb05_112Pyb05_112genetic_marker
Pyb05_113Pyb05_113genetic_marker
Pyb05_115Pyb05_115genetic_marker
Pyb05_119Pyb05_119genetic_marker
Pyb05_121Pyb05_121genetic_marker
Pyb05_124Pyb05_124genetic_marker
Pyb05_125Pyb05_125genetic_marker
Pyb05_126Pyb05_126genetic_marker
Pyb05_128Pyb05_128genetic_marker
Pyb05_129Pyb05_129genetic_marker
Pyb05_130Pyb05_130genetic_marker
Pyb05_131Pyb05_131genetic_marker
Pyb05_132Pyb05_132genetic_marker
Pyb05_135Pyb05_135genetic_marker
Pyb05_137Pyb05_137genetic_marker
Pyb05_138Pyb05_138genetic_marker
Pyb05_139Pyb05_139genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Pear-BD-F1-2014-genetic
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
DangshansuliDangshansulicultivar
Bayuehong_x_DangshansuliBayuehong_x_Dangshansulipopulation
BayuehongBayuehongcultivar
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:25129128
AGL: USDA National Agricultural LibraryAGL:4893819