Transferability of Newly Developed Pear SSR Markers to Other Rosaceae Species

Publication Overview
TitleTransferability of Newly Developed Pear SSR Markers to Other Rosaceae Species
AuthorsFan L, Zhang MY, Liu QZ, Li LT, Song Y, Wang LF, Zhang SL, Wu J
TypeJournal Article
Journal NamePlant molecular biology reporter / ISPMB
Volume31
Year2013
Page(s)1271-1282
CitationFan L, Zhang MY, Liu QZ, Li LT, Song Y, Wang LF, Zhang SL, Wu J. Transferability of Newly Developed Pear SSR Markers to Other Rosaceae Species. Plant molecular biology reporter / ISPMB. 2013; 31:1271-1282.

Abstract

A set of 120 simple sequence repeats (SSRs) was developed from the newly assembled pear sequence and evaluated for polymorphisms in seven genotypes of pear from different genetic backgrounds. Of these, 67 (55.8 %) primer pairs produced polymorphic amplifications. Together, the 67 SSRs detected 277 alleles with an average of 4.13 per locus. Sequencing of the amplification products from randomly picked loci NAUPy31a and NAUpy53a verified the presence of the SSR loci. When the 67 primer pairs were tested on 96 individual members of eight species in the Rosaceae family, 61.2 % (41/67) of the tested SSRs successfully amplified a PCR product in at least one of the Rosaceae genera. The transferability from pear to different species varied from 58.2 % (apple) to 11.9 % (cherry). The ratio of transferability also reflected the closer relationships within Maloideae over Prunoideae. Two pear SSR markers, NAUpy43c and NAUpy55k, could distinguish the 20 different apple genotypes thoroughly, and UPGMA cluster analysis grouped them into three groups at the similarity level of 0.56. The high level of polymorphism and good transferability of pear SSRs to Rosaceae species indicate their promise for application to future molecular screening, map construction, and comparative genomic studies among pears and other Rosaceae species.

Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:24415844
AGL: USDA National Agricultural LibraryAGL:4778520