High-density mapping suggests cytoplasmic male sterility with two restorer genes in almond × peach progenies

Publication Overview
TitleHigh-density mapping suggests cytoplasmic male sterility with two restorer genes in almond × peach progenies
AuthorsDonoso JM, Eduardo I, Picañol R, Batlle I, Howad W, Aranzana MJ, Arús P.
TypeJournal Article
Journal NameHorticulture Research
Volume2
Year2015
CitationDonoso JM, Eduardo I, Picañol R, Batlle I, Howad W, Aranzana MJ, Arús P. High-density mapping suggests cytoplasmic male sterility with two restorer genes in almond × peach progenies. Horticulture Research 2015 2: 15016

Abstract

Peach (Prunus persica) and almond (Prunus dulcis) are two sexually compatible species that produce fertile offspring. Almond, a highly polymorphic species, is a potential source of new genes for peach that has a strongly eroded gene pool. Here we describe the genetics of a male sterile phenotype that segregated in two almond (‘Texas’) × peach (‘Earlygold’) progenies: an F2 (T×E) and a backcross one (T1E) to the ‘Earlygold’ parent. High-density maps were developed using a 9k peach SNP chip and 135 simple-sequence repeats. Three highly syntenic and collinear maps were obtained: one for the F2 (T×E) and two for the backcross, T1E (for the hybrid) and E (for ‘Earlygold’). A major reduction of recombination was observed in the interspecific maps (T×E and T1E) compared to the intraspecific parent (E). The E map also had extensive monomorphic genomic regions suggesting the presence of large DNA fragments identical by descent. Our data for the male sterility character were consistent with the existence of cytoplasmic male sterility, where individuals having the almond cytoplasm required the almond allele in at least one of two independent restorer genes, Rf1 and Rf2, to be fertile. The restorer genes were located in a 3.4 Mbp fragment of linkage group 2 (Rf1) and 1.4 Mbp of linkage group 6 (Rf2). Both fragments contained several genes coding for pentatricopeptide proteins, demonstrated to be responsible for restoring fertility in other species. The implications of these results for using almond as a source of novel variability in peach are discussed.
File Attachments
AttachmentSize
File TxE_GDR.xlsx868.74 KB
File T1E_GDR.xlsx2.71 MB
File E_GDR.xlsx816.01 KB
File MS_GDR.xlsx1.47 MB
Features
This publication contains information about 480 features:
Feature NameUniquenameType
AMPA112AMPA112genetic_marker
BPPCT936BPPCT936genetic_marker
BPPCT937BPPCT937genetic_marker
BPPCT938BPPCT938genetic_marker
CPP20836CPP20836genetic_marker
CPP21245CPP21245genetic_marker
CPP21413CPP21413genetic_marker
CPP8062CPP8062genetic_marker
CPPCT011CPPCT011genetic_marker
CPPCT051CPPCT051genetic_marker
CPPCT053CPPCT053genetic_marker
CPPCT057CPPCT057genetic_marker
CPPCT371CPPCT371genetic_marker
CPPCT930CPPCT930genetic_marker
CPPCT944CPPCT944genetic_marker
CPSC1004CPSC1004genetic_marker
EPDCU0532EPDCU0532genetic_marker
EPDCU1945EPDCU1945genetic_marker
EPDCU2682EPDCU2682genetic_marker
EPDCU3832EPDCU3832genetic_marker
EPDCU4558EPDCU4558genetic_marker
EPDCU4610EPDCU4610genetic_marker
EPDCU4956EPDCU4956genetic_marker
EPPCU5178EPPCU5178genetic_marker
PaCITA21PaCITA21genetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Prunus-TE-F2-2015
Prunus-TE-BC1-T1E
Prunus-TE-BC1-E
Properties
Additional details for this publication include:
Property NameValue
URLhttp://www.nature.com/articles/hortres201516