The Peach v2.0 release: high-resolution linkage mapping and deep resequencing improve chromosome-scale assembly and contiguity

Publication Overview
TitleThe Peach v2.0 release: high-resolution linkage mapping and deep resequencing improve chromosome-scale assembly and contiguity
AuthorsVerde I, Jenkins J, Dondini L, Micali S, Pagliarani G, Vendramin E, Paris R, Aramini V, Gazza L, Rossini L, Bassi D, Troggio M, Shu S, Grimwood J, Tartarini S, Dettori MT, Schmutz J
TypeJournal Article
Journal NameBMC genomics
Volume18
Issue1
Year2017
Page(s)225
CitationVerde I, Jenkins J, Dondini L, Micali S, Pagliarani G, Vendramin E, Paris R, Aramini V, Gazza L, Rossini L, Bassi D, Troggio M, Shu S, Grimwood J, Tartarini S, Dettori MT, Schmutz J. The Peach v2.0 release: high-resolution linkage mapping and deep resequencing improve chromosome-scale assembly and contiguity. BMC genomics. 2017 Mar 11; 18(1):225.

Abstract

BACKGROUND
The availability of the peach genome sequence has fostered relevant research in peach and related Prunus species enabling the identification of genes underlying important horticultural traits as well as the development of advanced tools for genetic and genomic analyses. The first release of the peach genome (Peach v1.0) represented a high-quality WGS (Whole Genome Shotgun) chromosome-scale assembly with high contiguity (contig L50 214.2 kb), large portions of mapped sequences (96%) and high base accuracy (99.96%). The aim of this work was to improve the quality of the first assembly by increasing the portion of mapped and oriented sequences, correcting misassemblies and improving the contiguity and base accuracy using high-throughput linkage mapping and deep resequencing approaches.

RESULTS
Four linkage maps with 3,576 molecular markers were used to improve the portion of mapped and oriented sequences (from 96.0% and 85.6% of Peach v1.0 to 99.2% and 98.2% of v2.0, respectively) and enabled a more detailed identification of discernible misassemblies (10.4 Mb in total). The deep resequencing approach fixed 859 homozygous SNPs (Single Nucleotide Polymorphisms) and 1347 homozygous indels. Moreover, the assembled NGS contigs enabled the closing of 212 gaps with an improvement in the contig L50 of 19.2%.

CONCLUSIONS
The improved high quality peach genome assembly (Peach v2.0) represents a valuable tool for the analysis of the genetic diversity, domestication, and as a vehicle for genetic improvement of peach and related Prunus species. Moreover, the important phylogenetic position of peach and the absence of recent whole genome duplication (WGD) events make peach a pivotal species for comparative genomics studies aiming at elucidating plant speciation and diversification processes.

Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
Texas_x_Earligold-F2Texas_x_Earligold-F2population
IF7310828_x_Ferganensis-BC1IF7310828_x_Ferganensis-BC1population
Contender_x _Ambra-F2Contender_x _Ambra-F2population
Maria Dolce_x_SD81-F1Maria Dolce_x_SD81-F1population
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Pages

Featuremaps
This publication contains information about 6 maps:
Map Name
Prunus-TxE-peachV2.0
Peach-CA-BC1-2017
Peach-PF-BC1-2017
Peach-PF-F1-2017
Peach-PF-Recurrent-2017
Prunus-MDxSD-2017
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Journal CountryEngland
Journal AbbreviationBMC Genomics
DOI10.1186/s12864-017-3606-9
Elocation10.1186/s12864-017-3606-9
Publication ModelElectronic
ISSN1471-2164
eISSN1471-2164
Publication Date2017 Mar 11
LanguageEnglish
Language Abbreng
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:28284188