A cherry map from the inter-specific cross Prunus avium ‘Napoleon’ x P. nipponica based on microsatellite, gene-specific and isoenzyme markers

Publication Overview
TitleA cherry map from the inter-specific cross Prunus avium ‘Napoleon’ x P. nipponica based on microsatellite, gene-specific and isoenzyme markers
AuthorsClarke JB, Sargent DJ, Bošković RI, Belaj A, Tobutt KR
TypeJournal Article
Journal NameTree Genetics & Genomes
Volume5
Issue1
Year2009
Page(s)41-51
CitationClarke JB, Sargent DJ, Bošković RI, Belaj A, Tobutt KR. A cherry map from the inter-specific cross Prunus avium ‘Napoleon’ x P. nipponica based on microsatellite, gene-specific and isoenzyme markers. Tree Genetics & Genomes. 2009; 5(1):41-51.

Abstract

One hundred and sixty microsatellite (simple sequence repeat (SSR)) and six gene-specific markers revealing 174 loci were scored in 94 seedlings from the inter-specific cross of Prunus avium ‘Napoleon’ × Prunus nipponica accession F1292. The co-segregation data from these markers were used to construct a linkage map for cherry which spanned 680 cM over eight linkage groups with an average marker spacing of 3.9 cM per marker and just six gaps longer than 15 cM. Markers previously mapped in Prunus dulcis ‘Texas’ × Prunus persica ‘Earlygold’ allowed the cherry map to be anchored to the peach × almond map and showed the high level of synteny between the species. Eighty-four loci segregated in P. avium ‘Napoleon’ versus 159 in P. nipponica. The segregations of 32 isoenzyme loci in a subset of 47 seedlings from the progeny were scored, using polyacrylamide gel electrophoresis and/or isoelectric focusing separation followed by activity staining, and the co-segregation data were analysed along with those for 39 isoenzymes reported previously and for the 174 sequence-tagged site loci plus an additional two SSR loci. The second map incorporates 233 loci and spans 736 cM over eight linkage groups with an average marker spacing of 3.2 cM per marker and just two gaps greater than 15 cM. The microsatellite map will provide a useful tool for cherry breeding and marker-assisted selection and for synteny studies within Prunus; the gene-specific markers and isoenzymes will be useful for comparisons with maps of other rosaceous fruit crops.
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
NapoleonNapoleoncultivar
P._nipponicaP._nipponicacultivar
Napoleon_x_P._nipponicaNapoleon_x_P._nipponicacultivar
Features
This publication contains information about 16 features:
Feature NameUniquenameType
EMPA022EMPA022genetic_marker
EMPA026EMPA026genetic_marker
EMPA027EMPA027genetic_marker
EMPA029EMPA029genetic_marker
PS02A02PS02A02genetic_marker
PS09F09PS09F09genetic_marker
Pav_AltPav_Altgenetic_marker
Pav_CadPav_Cadgenetic_marker
Pav_FrkPav_Frkgenetic_marker
Pav_GlrPav_Glrgenetic_marker
Pav_MnrPav_Mnrgenetic_marker
Pav_NadpdhPav_Nadpdhgenetic_marker
Pav_NagPav_Naggenetic_marker
Pav_PepPav_Pepgenetic_marker
Pav_RbcPav_Rbcgenetic_marker
Pav_RnasePav_Rnasegenetic_marker
Featuremaps
This publication contains information about 2 maps:
Map Name
Sweet Cherry-PAxPN-94
Sweet Cherry-47
Properties
Additional details for this publication include:
Property NameValue
URLhttps://link.springer.com/article/10.1007/s11295-008-0166-9