DNA Fingerprinting of Closely Related Cultivars of Sweet Cherry

Publication Overview
TitleDNA Fingerprinting of Closely Related Cultivars of Sweet Cherry
AuthorsWiersma, P. A., Erogul, D., Ali, S.
TypeJournal Article
Journal NameJournal of the American Society for Horticultural Science
Volume143
Issue4
Year2018
Page(s)282-288
CitationWiersma, P. A., Erogul, D., Ali, S. (2018). "DNA Fingerprinting of Closely Related Cultivars of Sweet Cherry." Journal of the American Society for Horticultural Science 143(4): 282-288.

Abstract

Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were evaluated in an effort to reliably DNA fingerprint sweet cherry (Prunus avium L.) cultivars and advanced selections from the breeding program at the Summerland Research and Development Center (Summerland, BC, Canada). SSR markers were found that differentiated the 35 cultivars and selections tested. However, groups of cultivars closely related to the parental cultivars, Lapins and Sweetheart, were differentiated by only a few SSR markers each. These last few markers were discovered by specifically screening within these small groups of cultivars and the resulting markers had lower discriminating power (Dj) statistics within the full set of 35 cultivars and selections. To further characterize the differences in one of these closely related groups, SNP markers were identified in the cultivar Sweetheart and an analysis was made of how these markers segregated into three of its open-pollinated progeny. Large blocks of the ‘Sweetheart’ genome (34%) did not contain informative SNP markers, which was consistent with its ancestry where the cultivar Van is both a parent and grandparent. The three progeny cultivars differed from ‘Sweetheart’ at 14%, 31%, and 29% of the 3011 SNP positions tested. These were located in blocks of linked haplotypes covering from 2.5 to 20 million bps each and were distinct for the three cultivars. The cultivar Staccato, which required the most effort for SSR marker discrimination, also had the lowest number of SNP position differences from ‘Sweetheart’ (14%). These informative SNP markers were located in only five small regions of the sweet cherry genome, which also contained the discriminating SSR markers and provides an explanation for the difficulty of locating SSR markers for this cultivar. In addition to clearly differentiating these cultivars, this SNP analysis shows the level of variation expected within this closely related group.
Projects
This publication contains information about 1 projects:
Project NameDescription
Sweet_Cherry_Wiersma_2018
Features
This publication contains information about 3,011 features:
Feature NameUniquenameType
Pav_SUsnp1_0001Pav_SUsnp1_0001genetic_marker
Pav_SUsnp1_0002Pav_SUsnp1_0002genetic_marker
Pav_SUsnp1_0003Pav_SUsnp1_0003genetic_marker
Pav_SUsnp1_0004Pav_SUsnp1_0004genetic_marker
Pav_SUsnp1_0005Pav_SUsnp1_0005genetic_marker
Pav_SUsnp1_0006Pav_SUsnp1_0006genetic_marker
Pav_SUsnp1_0007Pav_SUsnp1_0007genetic_marker
Pav_SUsnp1_0008Pav_SUsnp1_0008genetic_marker
Pav_SUsnp1_0009Pav_SUsnp1_0009genetic_marker
Pav_SUsnp1_0010Pav_SUsnp1_0010genetic_marker
Pav_SUsnp1_0011Pav_SUsnp1_0011genetic_marker
Pav_SUsnp1_0012Pav_SUsnp1_0012genetic_marker
Pav_SUsnp1_0013Pav_SUsnp1_0013genetic_marker
Pav_SUsnp1_0014Pav_SUsnp1_0014genetic_marker
Pav_SUsnp1_0015Pav_SUsnp1_0015genetic_marker
Pav_SUsnp1_0016Pav_SUsnp1_0016genetic_marker
Pav_SUsnp1_0017Pav_SUsnp1_0017genetic_marker
Pav_SUsnp1_0018Pav_SUsnp1_0018genetic_marker
Pav_SUsnp1_0019Pav_SUsnp1_0019genetic_marker
Pav_SUsnp1_0020Pav_SUsnp1_0020genetic_marker
Pav_SUsnp1_0021Pav_SUsnp1_0021genetic_marker
Pav_SUsnp1_0022Pav_SUsnp1_0022genetic_marker
Pav_SUsnp1_0023Pav_SUsnp1_0023genetic_marker
Pav_SUsnp1_0024Pav_SUsnp1_0024genetic_marker
Pav_SUsnp1_0025Pav_SUsnp1_0025genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
DOI10.21273/JASHS04454-18