Identification of lipoxygenase (LOX) genes putatively involved in fruit flavour formation in apple (Malus × domestica)

Publication Overview
TitleIdentification of lipoxygenase (LOX) genes putatively involved in fruit flavour formation in apple (Malus × domestica)
AuthorsVogt J, Schiller D, Ulrich D, Schwab W, Dunemann F
TypeJournal Article
Journal NameTree genetics & genomes
Volume9
Issue6
Year2013
Page(s)1493-1511
CitationVogt J, Schiller D, Ulrich D, Schwab W, Dunemann F. Identification of lipoxygenase (LOX) genes putatively involved in fruit flavour formation in apple (Malus × domestica). Tree genetics & genomes. 2013; 9(6):1493-1511.

Abstract

Lipoxygenases (LOXs) are non-heme iron-containing enzymes that catalyse the dioxygenation of polyunsaturated fatty acids. The resulting hydroperoxides are further metabolized into biologically active oxylipins including jasmonic acid and green leaf volatiles (GLV) such as C6-aldehydes and C6-alcohols. LOXs are also known to play a decisive role in the production of volatiles that influence the flavour and aroma of fruits and vegetables. To obtain an overview of the inventory of the apple LOX gene family, the published Golden Delicious genome was mined for LOX coding sequences. In total, 23 putative functional LOX genes were identified and used for the construction of a phylogenetic tree. Two sub-trees were found which differentiate the LOX sequences into type 1- and type 2-LOXs. Their chromosomal locations were assigned to the predicted chromosomes of the assembled Golden Delicious genome sequence. Single LOX genes as well as clusters consisting of up to four genes were detected on apple chromosomes 2, 4, 5, 6, 7, 9, 11, 12, 13 and 16. LOX gene clusters on chromosomes 2 and 7, and on 4 and 12, respectively, indicated duplicated genome regions with high homology, which supports previous hypotheses of an ancient genome-wide duplication event in Malus. By using a PCR-based strategy, eight genes belonging to both type 1- and type 2-LOXs with altogether 30 full-length sequences were cloned. Several putative LOX alleles were detected within the same and among different apple cultivars. Two parental genetic maps available for ‘Discovery’ and ‘Prima’ were used for a quantitative trait locus (QTL) mapping experiment of apple volatile compounds known to be produced by the LOX biosynthetic pathway. The QTL detection resulted in a total number of 15 QTLs for eight volatiles (esters and the aldehyde hexanal) which were located on chromosomes 2, 7, 9 and 12 determined in silico as carriers of at least one LOX gene. To examine the putative roles of apple LOX genes in fruit volatile production, the spatial and temporal expression patterns were analysed by RT-PCR-based transcription analyses of apple leaf and fruit tissues. Two genes, MdLOX1a and MdLOX5e, were identified as candidate genes to be involved in fruit aroma volatile production in apple. The genetic association of QTLs found for the GLV hexanal at the top of chromosome 7, three clustered MdLOX5 genes with a putative 13-LOX function and published apple aphid resistance factors located all in the same region of chromosome 7 indicate that a lipoxygenase action might be involved in Malus aphid resistance reactions.
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
DiscoveryDiscoverycultivar
PrimaPrimacultivar
Discovery_x_PrimaDiscovery_x_Primapopulation
Projects
This publication contains information about 1 projects:
Project NameDescription
Apple-LOX-Dunemann-2013
Features
This publication contains information about 149 features:
Feature NameUniquenameType
MdLox1aMdLox1agenetic_marker
MdLox1dMdLox1dgenetic_marker
MdLox5aMdLox5agenetic_marker
MdLox5bMdLox5bgenetic_marker
MdLox5cMdLox5cgenetic_marker
MdLox5eMdLox5egenetic_marker
MdLox7aMdLox7agenetic_marker
lox1alox1agenetic_marker
lox2alox2agenetic_marker
lox2blox2bgenetic_marker
lox3alox3agenetic_marker
lox7alox7agenetic_marker
lox8alox8agenetic_marker
lox8blox8bgenetic_marker
lox9alox9agenetic_marker
E32M48-1E32M48-1genetic_marker
E32M48-12E32M48-12genetic_marker
E32M48-23E32M48-23genetic_marker
E32M48-8E32M48-8genetic_marker
E32M48-9E32M48-9genetic_marker
E32M50-12E32M50-12genetic_marker
E32M58-14E32M58-14genetic_marker
E32M58-18E32M58-18genetic_marker
E32M58-20E32M58-20genetic_marker
E32M58-4E32M58-4genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Apple-DP-F1-2013
Properties
Additional details for this publication include:
Property NameValue
URLhttp://dx.doi.org/10.1007/s11295-013-0653-5
Publication Model[electronic resource].
Language Abbreng
Published Location|||
Publication Date2013
Publication TypeJournal Article
KeywordsAphis pomi, Malus, alleles, apples, chromosomes, crop production, cultivars, esters, flavor, fruits, hydroperoxides, jasmonic acid, leaves, lipoxygenase, multigene family, nucleotide sequences, odors, oxylipins, phylogeny, polyunsaturated fatty acids, quantitative trait loci, vegetables, volatile compounds
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4786215