High-resolution mapping of the RMia gene for resistance to root-knot nematodes in peach

Publication Overview
TitleHigh-resolution mapping of the RMia gene for resistance to root-knot nematodes in peach
AuthorsDuval H, Hoerter M, Polidori J, Confolent C, Masse M, Moretti A, Van Ghelder C, Esmenjaud D
TypeJournal Article
Journal NameTree genetics & genomes
Volume10
Issue2
Year2014
Page(s)297-306
CitationDuval H, Hoerter M, Polidori J, Confolent C, Masse M, Moretti A, Van Ghelder C, Esmenjaud D. High-resolution mapping of the RMia gene for resistance to root-knot nematodes in peach. Tree genetics & genomes. 2014; 10(2):297-306.

Abstract

The RMia gene, which confers resistance (R) to the root-knot nematodes (RKN) Meloidogyne incognita and Meloidogyne arenaria, has been shown to segregate in the peach rootstocks Nemared, Shalil, and Juseitou on LG2 of the Prunus map. Here, we report the high-resolution mapping of RMia in Nemared, using the peach genome sequence and 790 individuals from two segregating peach populations, the F2 cross Montclar x Nemared and the four-way cross [(Pamirskij × Rubira) × (Montclar × Nemared)], in which Montclar, Pamirskij, and Rubira are susceptible (S) to RKN. Among the simple sequence repeat (SSR) markers designed for an initial flanking region of more than 1 Mb, five SSR markers specific for Nemared were characterized. The genotyping and phenotyping of recombinant individuals in this interval narrowed the gene’s location to a 300 kb physical distance between the SSR markers AMPP117 and AMPP116. In this interval, SNP polymorphisms were recovered from 1-kb-sequenced DNA fragments that were selected at 20 kb intervals. Two SNP markers (A20SNP and SNP_APP91) were shown to flank the gene in a final 92-kb region, containing four candidate genes from the TIR–NBS–LRR family. Finally, we studied the polymorphism of three closely linked markers, SNP_APP92, SNP_APP91, and AMPP117, on 28 R or S accessions from diverse Prunus species or hybrids. These markers discriminated between most R and S accessions, suggesting that at least the R sources of Nemared, Nemaguard, and Shalil share a common resistant ancestor.
Features
This publication contains information about 8 features:
Feature NameUniquenameType
AMPP115AMPP115genetic_marker
AMPP116AMPP116genetic_marker
AMPP117AMPP117genetic_marker
AMPP118AMPP118genetic_marker
AMPP119AMPP119genetic_marker
SNP_APP91SNP_APP91genetic_marker
SNP_APP92SNP_APP92genetic_marker
Meloidogine incognita resistanceRoot-knot nematode resistance-RMiaheritable_phenotypic_marker
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2014
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s11295-013-0683-z
KeywordsDNA, Meloidogyne arenaria, Meloidogyne incognita, Prunus, genes, genotyping, hybrids, microsatellite repeats, peaches, root-knot nematodes, single nucleotide polymorphism
Cross References
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DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4801179