Construction of genetic linkage maps and QTL mapping for X-disease resistance in tetraploid chokecherry (Prunus virginiana L.) using SSR and AFLP markers

Publication Overview
TitleConstruction of genetic linkage maps and QTL mapping for X-disease resistance in tetraploid chokecherry (Prunus virginiana L.) using SSR and AFLP markers
AuthorsWang H, Walla JA, Magnusson VA, Zhong S, Dai W
TypeJournal Article
Journal NameMolecular breeding
Volume34
Issue1
Year2014
Page(s)143-157
CitationWang H, Walla JA, Magnusson VA, Zhong S, Dai W. Construction of genetic linkage maps and QTL mapping for X-disease resistance in tetraploid chokecherry (Prunus virginiana L.) using SSR and AFLP markers. Molecular breeding. 2014; 34(1):143-157.

Abstract

Chokecherry (Prunus virginiana L.) (2n = 4x = 32) is a unique Prunus species for both genetics and disease resistance research due to its tetraploid nature and known variations in X-disease resistance. X-disease is a destructive disease of stone fruit trees, causing yield loss and poor fruit quality. However, genetic and genomic information on chokecherry is limited. In this study, simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers were used to construct genetic linkage maps and to identify quantitative trait loci (QTLs) associated with X-disease resistance in chokecherry. A segregating population (101 progenies) was developed by crossing an X-disease-resistant chokecherry line (RC) with a susceptible chokecherry line (SC). A total of 498 DNA markers (257 SSR and 241 AFLP markers) were mapped on the two genetic maps of the two parental lines (RC and SC). The map of RC contains 302 markers assigned to 14 linkage groups covering 2,089 cM of the genome. The map of SC has 259 markers assigned to 16 linkage groups covering 1,562.4 cM of the genome. The average distance between two markers was 6.9 cM for the RC map and 6.0 cM for the SC map. One QTL located on linkage group 15 on the map of SC was found to be associated with X-disease resistance. Genetic linkage maps and the identified QTL linked to X-disease resistance will further facilitate genetic research and breeding of X-disease resistance in chokecherry and other Prunus species.
Projects
This publication contains information about 1 projects:
Project NameDescription
Chokecherry X-disease resistance trait-Dai-2014
Featuremaps
This publication contains information about 1 maps:
Map Name
Chokecherry-RCxSC-F1-2014
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
R-II-2010-3R-II-2010-3breeding_research_material
S-V-2077-3S-V-2077-3breeding_research_material
RC_x_SC-F1RC_x_SC-F1population
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4825651