Fragaria Unigene v5.0

This is the fifth version of the Fragaria unigene. This build was used many sequencing projects around the world are depositing ESTs from the genus Fragaria in the NCBI dbEST database. The Fragaria ESTs included in this assembly were downloaded on July 1, 2012.

Not all of the Fragaria ESTs are of high quality. To filter, we crossmatched the public sequences against NCBI's UniVec database and used the BLAST sequence similarity algorithm to remove species-specific chloroplast, mitochondrial, tRNA, and rRNA sequences. To reduce redundancy and create longer transcripts we assembled these ESTs using the CAP3¹ program. The final assembly has been annotated by BLAST sequence similarity searching agaist Swiss-Prot²,TrEMBL³,TAIR⁴Arabidopsis proteinsPrunus persica⁵,Populus trichocarpa⁶ and Vitis vinifera⁷.

References

1. Huan, X. and Madan, A. (1999). CAP3: A DNA sequence assembly program. Genome Research, 9, 868-877.
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3. Boeckmann B., Bairoch A., Apweiler R., Blatter M.-C., Estreicher A., Gasteiger E., Martin M.J., Michoud K., O'Donovan C., Phan I., Pilbout S., and Sneider M. (2003) The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003. Nucleic Acids Research. 31:365-370.
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7. French-Italian Public Consortium for Grapevine Genome Characterization.(2007) The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature. Sep 27; 449(7161):463-7.