Rubus idaeus 'Joan J' Genome v2.0 Assembly & Annotation
Overview
Publication Zhou, J., Li, M., Li, Y., Xiao, Y., Luo, X., Gao, S., Ma, Z., Sadowski, N., Timp, W., Dardick, C., & [et al.]. (2023). Comparison of red raspberry and wild strawberry fruits reveals mechanisms of fruit type specification. Plant Physiology, kiad409. https://doi.org/10.1093/plphys/kiad409. Genome assembly
The final genome assembly has a total length of 297,436,202 bp with scaffold N50 of 33.74 Mb. 33,865 protein-coding genes were identified in the genome with a BUSCO completeness score of 96.5%. Homology
Homology of the Rubus idaeus JoanJ Genome v2.0 proteins was determined by pairwise sequence comparison using the blastp algorithm against various protein databases. An expectation value cutoff less than 1e-9 was used for the NCBI nr (Release 2021-09) and 1e-6 for the Arabidoposis proteins (Araport11), UniProtKB/SwissProt (Release 2021-09), and UniProtKB/TrEMBL (Release 2021-09) databases. The best hit reports are available for download in Excel format. Protein Homologs
Assembly
The Rubus idaeus 'Joan J' Genome v2.0 assembly file is available in FASTA format. Downloads
Gene Predictions
The Rubus idaeus 'Joan J' v2.0 genome gene prediction files are available in FASTA and GFF3 formats. Downloads
Functional Analysis
Functional annotation for the Rubus idaeus JoanJ Genome v2.0 are available for download below. The Rubus idaeus Genome v1.0 proteins were analyzed using InterProScan in order to assign InterPro domains and Gene Ontology (GO) terms. Pathways analysis was performed using the KEGG Automatic Annotation Server (KAAS). Downloads
Transcript Alignments
Transcript alignments were performed by the GDR Team of Main Bioinformatics Lab at WSU. The alignment tool 'BLAT' was used to map transcripts to the Rubus idaeus genome assembly. Alignments with an alignment length of 97% and 97% identify were preserved. The available files are in GFF3 format.
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