maker-Fvb7-4-snap-gene-64.46-mRNA-1, maker-Fvb7-4-snap-gene-64.46-mRNA-1_v1.0.a1 (mRNA) Fragaria x ananassa

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. TAIR10
Match: AT2G28930.2 (| Symbols: APK1B, PK1B | protein kinase 1B | chr2:12424551-12426565 FORWARD LENGTH=412)

HSP 1 Score: 437.573 bits (1124), Expect = 6.315e-146
Identity = 208/363 (57.30%), Postives = 270/363 (74.38%), Query Frame = 0

Query:  523 VPSTPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYI 881
            + + P  + +IL    L+ F + ELK AT NF P++ LGEG FG VFKGW+D+ T TA+KPGT +++AVK +N++G QGH+EWL E+ YLG+  HPN+VKL+GYC ED+HRLLVYEFM  GSLE HLF   SY QPLSW LR+K+ALGAAK LAF+H++E  VI+RDFK+SNILLDS YNAKLSDFG AKDGP GD SHVSTR+MGTYGYAAPEY+ATGHLT K+DVY +GVV+LE+LSGR+ +D +RP  EQ LVEW++P LA+KR++ ++ D R++ QYS+  A K A LA RCL+ E +LRPNMNEVV  LE +Q     E  G +++ +++ +   S+S   + K    F    + G I
Sbjct:   38 IRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQTLN--EAGGRNIDMVQRRMRRRSDSVAINQKPNAGFARQTAVGVI 398          

HSP 2 Score: 415.231 bits (1066), Expect = 2.519e-137
Identity = 203/324 (62.65%), Postives = 247/324 (76.23%), Query Frame = 0

Query:  115 VPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQ 430
            + +NP+T+ EIL    LKSF + ELKAATRNFRPD+VLGEGGFG V+KGWID+ +L AS+PGTG+ +A+K+L+QD  QG +EW  EV YLGQ  HPNLVKLIGYC ED   LLVYEF+PRGSLEN+LFR  S F  +PLSW  R+K+ALGAAK LAFLHN E  +I+RDFK+SNILLDS YN KLSDFGL  DG +    G K+ V   I+GTYGYAAPEY+ATG LTTK DVY +GVV+LE+LSG+R  DKNRPPGEQ LVEWA+P L  K ++ ++ D R++ Q+S+  A K   LA +CL+   KLRPNMN VV  LE +Q
Sbjct:   38 IRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYF--QPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPT----GDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQ 355          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. TAIR10
Match: AT2G28930.3 (| Symbols: APK1B, PK1B | protein kinase 1B | chr2:12424551-12426565 FORWARD LENGTH=415)

HSP 1 Score: 437.187 bits (1123), Expect = 1.075e-145
Identity = 208/363 (57.30%), Postives = 270/363 (74.38%), Query Frame = 0

Query:  523 VPSTPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYI 881
            + + P  + +IL    L+ F + ELK AT NF P++ LGEG FG VFKGW+D+ T TA+KPGT +++AVK +N++G QGH+EWL E+ YLG+  HPN+VKL+GYC ED+HRLLVYEFM  GSLE HLF   SY QPLSW LR+K+ALGAAK LAF+H++E  VI+RDFK+SNILLDS YNAKLSDFG AKDGP GD SHVSTR+MGTYGYAAPEY+ATGHLT K+DVY +GVV+LE+LSGR+ +D +RP  EQ LVEW++P LA+KR++ ++ D R++ QYS+  A K A LA RCL+ E +LRPNMNEVV  LE +Q     E  G +++ +++ +   S+S   + K    F    + G I
Sbjct:   41 IRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQTLN--EAGGRNIDMVQRRMRRRSDSVAINQKPNAGFARQTAVGVI 401          

HSP 2 Score: 414.846 bits (1065), Expect = 4.564e-137
Identity = 203/324 (62.65%), Postives = 247/324 (76.23%), Query Frame = 0

Query:  115 VPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQ 430
            + +NP+T+ EIL    LKSF + ELKAATRNFRPD+VLGEGGFG V+KGWID+ +L AS+PGTG+ +A+K+L+QD  QG +EW  EV YLGQ  HPNLVKLIGYC ED   LLVYEF+PRGSLEN+LFR  S F  +PLSW  R+K+ALGAAK LAFLHN E  +I+RDFK+SNILLDS YN KLSDFGL  DG +    G K+ V   I+GTYGYAAPEY+ATG LTTK DVY +GVV+LE+LSG+R  DKNRPPGEQ LVEWA+P L  K ++ ++ D R++ Q+S+  A K   LA +CL+   KLRPNMN VV  LE +Q
Sbjct:   41 IRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYF--QPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPT----GDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQ 358          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. TAIR10
Match: AT2G28930.1 (| Symbols: APK1B, PK1B | protein kinase 1B | chr2:12424957-12426565 FORWARD LENGTH=423)

HSP 1 Score: 436.417 bits (1121), Expect = 2.341e-145
Identity = 208/363 (57.30%), Postives = 270/363 (74.38%), Query Frame = 0

Query:  523 VPSTPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYI 881
            + + P  + +IL    L+ F + ELK AT NF P++ LGEG FG VFKGW+D+ T TA+KPGT +++AVK +N++G QGH+EWL E+ YLG+  HPN+VKL+GYC ED+HRLLVYEFM  GSLE HLF   SY QPLSW LR+K+ALGAAK LAF+H++E  VI+RDFK+SNILLDS YNAKLSDFG AKDGP GD SHVSTR+MGTYGYAAPEY+ATGHLT K+DVY +GVV+LE+LSGR+ +D +RP  EQ LVEW++P LA+KR++ ++ D R++ QYS+  A K A LA RCL+ E +LRPNMNEVV  LE +Q     E  G +++ +++ +   S+S   + K    F    + G I
Sbjct:   49 IRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQTLN--EAGGRNIDMVQRRMRRRSDSVAINQKPNAGFARQTAVGVI 409          

HSP 2 Score: 414.075 bits (1063), Expect = 1.113e-136
Identity = 203/324 (62.65%), Postives = 247/324 (76.23%), Query Frame = 0

Query:  115 VPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQ 430
            + +NP+T+ EIL    LKSF + ELKAATRNFRPD+VLGEGGFG V+KGWID+ +L AS+PGTG+ +A+K+L+QD  QG +EW  EV YLGQ  HPNLVKLIGYC ED   LLVYEF+PRGSLEN+LFR  S F  +PLSW  R+K+ALGAAK LAFLHN E  +I+RDFK+SNILLDS YN KLSDFGL  DG +    G K+ V   I+GTYGYAAPEY+ATG LTTK DVY +GVV+LE+LSG+R  DKNRPPGEQ LVEWA+P L  K ++ ++ D R++ Q+S+  A K   LA +CL+   KLRPNMN VV  LE +Q
Sbjct:   49 IRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYF--QPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPT----GDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQ 366          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. TAIR10
Match: AT1G07570.1 (| Symbols: APK1A, APK1 | Protein kinase superfamily protein | chr1:2331369-2333210 REVERSE LENGTH=410)

HSP 1 Score: 427.557 bits (1098), Expect = 4.012e-142
Identity = 200/325 (61.54%), Postives = 256/325 (78.77%), Query Frame = 0

Query:  516 SMFSICDVPSTPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQ 836
            S  S   V  +P  + +IL    L+ F + ELK+AT NF P++ LGEG FG VFKGW+D+ + TA++PGT +++AVK +N++G QGH+EWL E+ YLG+  H ++VKL+GYC ED+HRLLVYEFM  GSLE HLF    Y QPLSW LR+K+ALGAAK LAF+HSSE +VI+RDFK+SNILLDS YNAKLSDFG AKDGP GD SHVSTRVMGT+GYAAPEY+ATGHLT K+DVY FGVV+LELLSGR+ +D +RP+ E++LVEW+KPYL +KR++ ++ D R++ QYS+  A K A L+ RCL+ E +LRPNM+EVV  LE +Q
Sbjct:   30 SKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQ 354          

HSP 2 Score: 403.675 bits (1036), Expect = 6.494e-133
Identity = 202/329 (61.40%), Postives = 248/329 (75.38%), Query Frame = 0

Query:  107 RAESLPYRVPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLH-NEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQ 430
            +A S+  R PS P+T+ EIL    LKSF + ELK+ATRNFRPD+VLGEGGFG V+KGWID+ SL AS PGTG+ +A+K+L+QD  QG +EW  EV YLGQ  H +LVKLIGYC ED   LLVYEF+PRGSLEN+LFR    F  +PLSW  R+K+ALGAAK LAFLH +E ++I+RDFK+SNILLDS YN KLSDFGL  DG         T+V +GT+GYAAPEY+ATG LTTK DVY FGVV+LELLSG+R  DKNRP GE+NLVEWAKPYLV K ++ ++ D R++ Q+S+  A K   L+ +CL+   KLRPNM+ VV  LE +Q
Sbjct:   31 KASSVSVR-PS-PRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYF--QPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRV-MGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQ 354          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. TAIR10
Match: AT1G07570.2 (| Symbols: APK1A, APK1 | Protein kinase superfamily protein | chr1:2331369-2333210 REVERSE LENGTH=410)

HSP 1 Score: 427.557 bits (1098), Expect = 4.012e-142
Identity = 200/325 (61.54%), Postives = 256/325 (78.77%), Query Frame = 0

Query:  516 SMFSICDVPSTPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQ 836
            S  S   V  +P  + +IL    L+ F + ELK+AT NF P++ LGEG FG VFKGW+D+ + TA++PGT +++AVK +N++G QGH+EWL E+ YLG+  H ++VKL+GYC ED+HRLLVYEFM  GSLE HLF    Y QPLSW LR+K+ALGAAK LAF+HSSE +VI+RDFK+SNILLDS YNAKLSDFG AKDGP GD SHVSTRVMGT+GYAAPEY+ATGHLT K+DVY FGVV+LELLSGR+ +D +RP+ E++LVEW+KPYL +KR++ ++ D R++ QYS+  A K A L+ RCL+ E +LRPNM+EVV  LE +Q
Sbjct:   30 SKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQ 354          

HSP 2 Score: 403.675 bits (1036), Expect = 6.494e-133
Identity = 202/329 (61.40%), Postives = 248/329 (75.38%), Query Frame = 0

Query:  107 RAESLPYRVPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLH-NEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQ 430
            +A S+  R PS P+T+ EIL    LKSF + ELK+ATRNFRPD+VLGEGGFG V+KGWID+ SL AS PGTG+ +A+K+L+QD  QG +EW  EV YLGQ  H +LVKLIGYC ED   LLVYEF+PRGSLEN+LFR    F  +PLSW  R+K+ALGAAK LAFLH +E ++I+RDFK+SNILLDS YN KLSDFGL  DG         T+V +GT+GYAAPEY+ATG LTTK DVY FGVV+LELLSG+R  DKNRP GE+NLVEWAKPYLV K ++ ++ D R++ Q+S+  A K   L+ +CL+   KLRPNM+ VV  LE +Q
Sbjct:   31 KASSVSVR-PS-PRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYF--QPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRV-MGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQ 354          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. TAIR10
Match: AT1G07570.3 (| Symbols: APK1A | Protein kinase superfamily protein | chr1:2331369-2333589 REVERSE LENGTH=424)

HSP 1 Score: 426.402 bits (1095), Expect = 1.789e-141
Identity = 200/325 (61.54%), Postives = 256/325 (78.77%), Query Frame = 0

Query:  516 SMFSICDVPSTPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQ 836
            S  S   V  +P  + +IL    L+ F + ELK+AT NF P++ LGEG FG VFKGW+D+ + TA++PGT +++AVK +N++G QGH+EWL E+ YLG+  H ++VKL+GYC ED+HRLLVYEFM  GSLE HLF    Y QPLSW LR+K+ALGAAK LAF+HSSE +VI+RDFK+SNILLDS YNAKLSDFG AKDGP GD SHVSTRVMGT+GYAAPEY+ATGHLT K+DVY FGVV+LELLSGR+ +D +RP+ E++LVEW+KPYL +KR++ ++ D R++ QYS+  A K A L+ RCL+ E +LRPNM+EVV  LE +Q
Sbjct:   44 SKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQ 368          

HSP 2 Score: 402.905 bits (1034), Expect = 2.633e-132
Identity = 198/321 (61.68%), Postives = 243/321 (75.70%), Query Frame = 0

Query:  115 VPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLH-NEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQ 430
            V  +P+T+ EIL    LKSF + ELK+ATRNFRPD+VLGEGGFG V+KGWID+ SL AS PGTG+ +A+K+L+QD  QG +EW  EV YLGQ  H +LVKLIGYC ED   LLVYEF+PRGSLEN+LFR    F  +PLSW  R+K+ALGAAK LAFLH +E ++I+RDFK+SNILLDS YN KLSDFGL  DG         T+V +GT+GYAAPEY+ATG LTTK DVY FGVV+LELLSG+R  DKNRP GE+NLVEWAKPYLV K ++ ++ D R++ Q+S+  A K   L+ +CL+   KLRPNM+ VV  LE +Q
Sbjct:   51 VRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYF--QPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRV-MGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQ 368          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. TAIR10
Match: AT5G02290.2 (| Symbols: NAK | Protein kinase superfamily protein | chr5:470387-472397 REVERSE LENGTH=389)

HSP 1 Score: 413.69 bits (1062), Expect = 4.493e-137
Identity = 188/314 (59.87%), Postives = 252/314 (80.25%), Query Frame = 0

Query:  527 PPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQ 836
            P  + +IL    L+ F  +ELK+AT NF P++ +GEG FG VFKGW+D+++   +KPGT +++AVK +N+EG QGH EWL EI YLG+L HPN+VKL+GYC E++HRLLVYEFM  GSLE HLF   ++ QPLSW  R+++ALGAA+ LAF+H+++ +VI+RDFK+SNILLDSNYNAKLSDFG A+DGP GD+SHVSTRVMGT GYAAPEY+ATGHL+ K+DVY FGVV+LELLSGR+ +D ++P  E +LV+W++PYL +KRR+L++ D R++GQYS+  A K A LA  C+S++ + RP MNE+VK +E+L 
Sbjct:   41 PRTEGEILQNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKRLNQEGFQGHREWLAEINYLGQLDHPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFYQPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDVYSFGVVLLELLSGRRAIDKNQPVGEHNLVDWARPYLTNKRRLLRVMDPRLQGQYSLTRALKIAVLALDCISIDAKSRPTMNEIVKTMEELH 354          

HSP 2 Score: 399.823 bits (1026), Expect = 1.045e-131
Identity = 196/334 (58.68%), Postives = 254/334 (76.05%), Query Frame = 0

Query:  119 PQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKL----QQSSDRDDLRISQD 443
            P+T+ EIL    LK+F  +ELK+ATRNFRPD+V+GEGGFG V+KGWID++SLA S+PGTG+ +A+KRL+Q+  QG  EW  E+ YLGQL HPNLVKLIGYC E+   LLVYEF+ RGSLEN+LFR  + +  +PLSWN R+++ALGAA+ LAFLHN + Q+I+RDFK+SNILLDS YN KLSDFGL  DG    +    T+V +GT GYAAPEY+ATG L+ K DVY FGVV+LELLSG+R  DKN+P GE NLV+WA+PYL  K R+L++ D R++GQ+S+ RALK   LA  C+S + K RP MN +VK +E+L    + S ++ + +IS D
Sbjct:   41 PRTEGEILQNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKRLNQEGFQGHREWLAEINYLGQLDHPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFY--QPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRV-MGTQGYAAPEYLATGHLSVKSDVYSFGVVLLELLSGRRAIDKNQPVGEHNLVDWARPYLTNKRRLLRVMDPRLQGQYSLTRALKIAVLALDCISIDAKSRPTMNEIVKTMEELHIQKEASKEQQNPQISID 371          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. TAIR10
Match: AT5G02290.1 (| Symbols: NAK | Protein kinase superfamily protein | chr5:470387-472397 REVERSE LENGTH=389)

HSP 1 Score: 413.69 bits (1062), Expect = 4.493e-137
Identity = 188/314 (59.87%), Postives = 252/314 (80.25%), Query Frame = 0

Query:  527 PPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQ 836
            P  + +IL    L+ F  +ELK+AT NF P++ +GEG FG VFKGW+D+++   +KPGT +++AVK +N+EG QGH EWL EI YLG+L HPN+VKL+GYC E++HRLLVYEFM  GSLE HLF   ++ QPLSW  R+++ALGAA+ LAF+H+++ +VI+RDFK+SNILLDSNYNAKLSDFG A+DGP GD+SHVSTRVMGT GYAAPEY+ATGHL+ K+DVY FGVV+LELLSGR+ +D ++P  E +LV+W++PYL +KRR+L++ D R++GQYS+  A K A LA  C+S++ + RP MNE+VK +E+L 
Sbjct:   41 PRTEGEILQNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKRLNQEGFQGHREWLAEINYLGQLDHPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFYQPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDVYSFGVVLLELLSGRRAIDKNQPVGEHNLVDWARPYLTNKRRLLRVMDPRLQGQYSLTRALKIAVLALDCISIDAKSRPTMNEIVKTMEELH 354          

HSP 2 Score: 399.823 bits (1026), Expect = 1.045e-131
Identity = 196/334 (58.68%), Postives = 254/334 (76.05%), Query Frame = 0

Query:  119 PQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKL----QQSSDRDDLRISQD 443
            P+T+ EIL    LK+F  +ELK+ATRNFRPD+V+GEGGFG V+KGWID++SLA S+PGTG+ +A+KRL+Q+  QG  EW  E+ YLGQL HPNLVKLIGYC E+   LLVYEF+ RGSLEN+LFR  + +  +PLSWN R+++ALGAA+ LAFLHN + Q+I+RDFK+SNILLDS YN KLSDFGL  DG    +    T+V +GT GYAAPEY+ATG L+ K DVY FGVV+LELLSG+R  DKN+P GE NLV+WA+PYL  K R+L++ D R++GQ+S+ RALK   LA  C+S + K RP MN +VK +E+L    + S ++ + +IS D
Sbjct:   41 PRTEGEILQNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKRLNQEGFQGHREWLAEINYLGQLDHPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFY--QPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRV-MGTQGYAAPEYLATGHLSVKSDVYSFGVVLLELLSGRRAIDKNQPVGEHNLVDWARPYLTNKRRLLRVMDPRLQGQYSLTRALKIAVLALDCISIDAKSRPTMNEIVKTMEELHIQKEASKEQQNPQISID 371          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. TAIR10
Match: AT2G39660.1 (| Symbols: BIK1 | botrytis-induced kinase1 | chr2:16531943-16533601 FORWARD LENGTH=395)

HSP 1 Score: 412.149 bits (1058), Expect = 2.693e-136
Identity = 188/310 (60.65%), Postives = 247/310 (79.68%), Query Frame = 0

Query:  529 IQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQES 838
            I S   ++ F +NELK AT NF P++ +GEG FG VFKGW+D++T T  KPGT +++AVK +N+EG QGH EWLTEI YLG+L HPN+VKL+GYC ED+HRLLVYEFM  GSLE HLF   +Y +PL W LR+ +AL AAK LAF+HS   KVI+RD K+SNILLD++YNAKLSDFG A+DGP GD S+VSTRVMGTYGYAAPEY+++GHL  ++DVY FGV++LE+LSG++ LD +RP +E++LV+W++PYL SKR+VL I D R+  QY    A + A++A +CLS EP+ RP M++VV+AL+QLQ++
Sbjct:   46 ILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKGWLDESTLTPTKPGTGLVIAVKKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEHRLLVYEFMQKGSLENHLFRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAKLSDFGLARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNARSDVYSFGVLLLEILSGKRALDHNRPAKEENLVDWARPYLTSKRKVLLIVDNRLDTQYLPEEAVRMASVAVQCLSFEPKSRPTMDQVVRALQQLQDN 355          

HSP 2 Score: 371.318 bits (952), Expect = 1.295e-120
Identity = 192/380 (50.53%), Postives = 256/380 (67.37%), Query Frame = 0

Query:   97 KSRDSNGTRIRAESLPYRVPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNE-AQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSRNGIGTMSGGIRTSVKGF 471
            KS D  G  + +      V +  +T+ EIL    +KSF +NELK ATRNFRPD+V+GEGGFG V+KGW+D+++L  ++PGTG+ +A+K+L+Q+  QG  EW  E+ YLGQL HPNLVKLIGYC ED   LLVYEF+ +GSLEN+LFR  + F  +PL W  R+ +AL AAK LAFLH++  ++I+RD K+SNILLD+ YN KLSDFGL  DG         T+V +GTYGYAAPEY+++G L  + DVY FGV++LE+LSGKR  D NRP  E+NLV+WA+PYL  K +VL I D R+  Q+    A++   +A QCLS  PK RP M+ VV+AL++LQ     D+L     KP Q         +G  +G  ++S K F
Sbjct:   18 KSSDLYGLSLSSRKSSSTVAAAQKTEGEILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKGWLDESTLTPTKPGTGLVIAVKKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEHRLLVYEFMQKGSLENHLFRRGAYF--KPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAKLSDFGLARDGPMGDLSYVSTRV-MGTYGYAAPEYMSSGHLNARSDVYSFGVLLLEILSGKRALDHNRPAKEENLVDWARPYLTSKRKVLLIVDNRLDTQYLPEEAVRMASVAVQCLSFEPKSRPTMDQVVRALQQLQ-----DNL----GKPSQTNPVKDTKKLGFKTGTTKSSEKRF 385          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. TAIR10
Match: AT3G55450.1 (| Symbols: PBL1 | PBS1-like 1 | chr3:20558129-20559963 FORWARD LENGTH=389)

HSP 1 Score: 397.127 bits (1019), Expect = 1.250e-130
Identity = 186/311 (59.81%), Postives = 241/311 (77.49%), Query Frame = 0

Query:  529 IQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSY-LQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQES 838
            I S   ++ F +NELK AT NF  ++ +GEG FG VF+GW+D+ T T  K  + +++AVK +N +G QGH EWLTEI YLG+L HPN+VKL+GYC ED+ RLLVYEFM  GSLE HLF   +   +PLSWILR+K+AL AAK LAF+HS   KVI+RD K+SNILLDS++NAKLSDFG A+DGP G+ S+VSTRVMGT+GYAAPEYV+TGHL  ++DVY FGVV+LELL GR+ LD +RP +EQ+LV+W++PYL S+R+VL I D R+  QY    A + A++A +CLS EP+ RP M++VV+AL QLQ+S
Sbjct:   40 ILSSTTVKSFSFNELKLATRNFRSDSVVGEGGFGCVFRGWLDETTLTPTKSSSGLVIAVKRLNPDGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEQRLLVYEFMHKGSLENHLFANGNKDFKPLSWILRIKVALDAAKGLAFLHSDPVKVIYRDIKASNILLDSDFNAKLSDFGLARDGPMGEQSYVSTRVMGTFGYAAPEYVSTGHLNARSDVYSFGVVLLELLCGRQALDHNRPAKEQNLVDWARPYLTSRRKVLLIVDTRLNSQYKPEGAVRLASIAVQCLSFEPKSRPTMDQVVRALVQLQDS 350          

HSP 2 Score: 361.303 bits (926), Expect = 8.353e-117
Identity = 181/357 (50.70%), Postives = 245/357 (68.63%), Query Frame = 0

Query:  120 QTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNE-AQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQS----SDRDDLRISQDKP---CQNAQASSRNGIGTMSGGI 464
            +T+ EIL    +KSF +NELK ATRNFR D+V+GEGGFG V++GW+D+ +L  ++  +G+ +A+KRL+ D  QG  EW  E+ YLGQL HPNLVKLIGYC ED + LLVYEF+ +GSLEN+LF  + N + +PLSW  R+K+AL AAK LAFLH++  ++I+RD K+SNILLDS +N KLSDFGL  DG         T+V +GT+GYAAPEY++TG L  + DVY FGVV+LELL G++  D NRP  EQNLV+WA+PYL  + +VL I D R+  Q+    A++   +A QCLS  PK RP M+ VV+AL +LQ S    ++ D L++   K     +      RNG+   + G+
Sbjct:   35 KTEGEILSSTTVKSFSFNELKLATRNFRSDSVVGEGGFGCVFRGWLDETTLTPTKSSSGLVIAVKRLNPDGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEQRLLVYEFMHKGSLENHLF-ANGNKDFKPLSWILRIKVALDAAKGLAFLHSDPVKVIYRDIKASNILLDSDFNAKLSDFGLARDGPMGEQSYVSTRV-MGTFGYAAPEYVSTGHLNARSDVYSFGVVLLELLCGRQALDHNRPAKEQNLVDWARPYLTSRRKVLLIVDTRLNSQYKPEGAVRLASIAVQCLSFEPKSRPTMDQVVRALVQLQDSVVKPANVDPLKVKDTKKLVGLKTEDKYQRNGLNKKTVGL 389          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy Swiss-Prot
Match: RK176_ORYSJ (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 451.44 bits (1160), Expect = 1.991e-150
Identity = 217/348 (62.36%), Postives = 269/348 (77.30%), Query Frame = 0

Query:  524 PSTPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWK 867
            P TP  + +IL    ++ F +NEL+TAT NF P++ LGEG FG VFKGW+D+ T    KPGT M++AVK +N+EG QGH EWL E+ YLG+L HP +V+L+GYC ED+ RLLVYEFM  GSLE HLF   ++ QPLSW LRMKIALGAAK LAF+HS + KVI+RDFK+SN+LLD+NY+AKLSDFG AKDGP GD SHVSTRVMGTYGYAAPEY+ATGHLT K+DVY FGVV+LE+LSGR+ LD +RPT E +LVEW++PYL SKRR+ +I DAR+ GQYS+A A+KAA LA +C+SVE + RPNM +VV  LEQLQ+S    G+   L +   S  + SN +K S K
Sbjct:   40 PPTPRSEDEILEAANVKAFAFNELRTATRNFRPDSVLGEGGFGSVFKGWIDEKTLAPTKPGTGMVIAVKKLNQEGHQGHREWLAEVNYLGQLSHPYLVRLVGYCVEDEQRLLVYEFMPRGSLENHLFRRSTHFQPLSWNLRMKIALGAAKGLAFLHSDKVKVIYRDFKTSNVLLDANYDAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLTTKSDVYSFGVVLLEMLSGRRALDKNRPTGEHNLVEWARPYLMSKRRIFRILDARLGGQYSLAKAQKAATLALQCISVEAKNRPNMEQVVAVLEQLQDSKET-GANPQLQKKSSSKNAGSNGSKPSSK 386          

HSP 2 Score: 423.705 bits (1088), Expect = 1.054e-139
Identity = 209/360 (58.06%), Postives = 267/360 (74.17%), Query Frame = 0

Query:  116 PSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLH-NEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSRNGIGTMSGGIRTSVKG 470
            P  P+++ EIL    +K+F +NEL+ ATRNFRPD+VLGEGGFG V+KGWID+ +LA ++PGTGM +A+K+L+Q+  QG  EW  EV YLGQL HP LV+L+GYC ED + LLVYEF+PRGSLEN+LFR S++F  +PLSWN RMKIALGAAK LAFLH ++ ++I+RDFK+SN+LLD+ Y+ KLSDFGL  DG +       T+V +GTYGYAAPEY+ATG LTTK DVY FGVV+LE+LSG+R  DKNRP GE NLVEWA+PYL+ K R+ +I DAR+ GQ+S+A+A KA  LA QC+S   K RPNM  VV  LE+LQ S +      +   P    ++SS+N     S G + S KG
Sbjct:   40 PPTPRSEDEILEAANVKAFAFNELRTATRNFRPDSVLGEGGFGSVFKGWIDEKTLAPTKPGTGMVIAVKKLNQEGHQGHREWLAEVNYLGQLSHPYLVRLVGYCVEDEQRLLVYEFMPRGSLENHLFRRSTHF--QPLSWNLRMKIALGAAKGLAFLHSDKVKVIYRDFKTSNVLLDANYDAKLSDFGLAKDGPTGDKSHVSTRV-MGTYGYAAPEYLATGHLTTKSDVYSFGVVLLEMLSGRRALDKNRPTGEHNLVEWARPYLMSKRRIFRILDARLGGQYSLAKAQKAATLALQCISVEAKNRPNMEQVVAVLEQLQDSKE------TGANPQLQKKSSSKN---AGSNGSKPSSKG 387          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy Swiss-Prot
Match: PBL10_ARATH (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 437.573 bits (1124), Expect = 8.754e-145
Identity = 208/363 (57.30%), Postives = 270/363 (74.38%), Query Frame = 0

Query:  523 VPSTPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYI 881
            + + P  + +IL    L+ F + ELK AT NF P++ LGEG FG VFKGW+D+ T TA+KPGT +++AVK +N++G QGH+EWL E+ YLG+  HPN+VKL+GYC ED+HRLLVYEFM  GSLE HLF   SY QPLSW LR+K+ALGAAK LAF+H++E  VI+RDFK+SNILLDS YNAKLSDFG AKDGP GD SHVSTR+MGTYGYAAPEY+ATGHLT K+DVY +GVV+LE+LSGR+ +D +RP  EQ LVEW++P LA+KR++ ++ D R++ QYS+  A K A LA RCL+ E +LRPNMNEVV  LE +Q     E  G +++ +++ +   S+S   + K    F    + G I
Sbjct:   38 IRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQTLN--EAGGRNIDMVQRRMRRRSDSVAINQKPNAGFARQTAVGVI 398          

HSP 2 Score: 415.231 bits (1066), Expect = 3.492e-136
Identity = 203/324 (62.65%), Postives = 247/324 (76.23%), Query Frame = 0

Query:  115 VPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQ 430
            + +NP+T+ EIL    LKSF + ELKAATRNFRPD+VLGEGGFG V+KGWID+ +L AS+PGTG+ +A+K+L+QD  QG +EW  EV YLGQ  HPNLVKLIGYC ED   LLVYEF+PRGSLEN+LFR  S F  +PLSW  R+K+ALGAAK LAFLHN E  +I+RDFK+SNILLDS YN KLSDFGL  DG +    G K+ V   I+GTYGYAAPEY+ATG LTTK DVY +GVV+LE+LSG+R  DKNRPPGEQ LVEWA+P L  K ++ ++ D R++ Q+S+  A K   LA +CL+   KLRPNMN VV  LE +Q
Sbjct:   38 IRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYF--QPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPT----GDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQ 355          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy Swiss-Prot
Match: PBL9_ARATH (Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN=PBL9 PE=1 SV=1)

HSP 1 Score: 427.557 bits (1098), Expect = 5.561e-141
Identity = 200/325 (61.54%), Postives = 256/325 (78.77%), Query Frame = 0

Query:  516 SMFSICDVPSTPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQ 836
            S  S   V  +P  + +IL    L+ F + ELK+AT NF P++ LGEG FG VFKGW+D+ + TA++PGT +++AVK +N++G QGH+EWL E+ YLG+  H ++VKL+GYC ED+HRLLVYEFM  GSLE HLF    Y QPLSW LR+K+ALGAAK LAF+HSSE +VI+RDFK+SNILLDS YNAKLSDFG AKDGP GD SHVSTRVMGT+GYAAPEY+ATGHLT K+DVY FGVV+LELLSGR+ +D +RP+ E++LVEW+KPYL +KR++ ++ D R++ QYS+  A K A L+ RCL+ E +LRPNM+EVV  LE +Q
Sbjct:   30 SKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQ 354          

HSP 2 Score: 403.675 bits (1036), Expect = 9.001e-132
Identity = 202/329 (61.40%), Postives = 248/329 (75.38%), Query Frame = 0

Query:  107 RAESLPYRVPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLH-NEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQ 430
            +A S+  R PS P+T+ EIL    LKSF + ELK+ATRNFRPD+VLGEGGFG V+KGWID+ SL AS PGTG+ +A+K+L+QD  QG +EW  EV YLGQ  H +LVKLIGYC ED   LLVYEF+PRGSLEN+LFR    F  +PLSW  R+K+ALGAAK LAFLH +E ++I+RDFK+SNILLDS YN KLSDFGL  DG         T+V +GT+GYAAPEY+ATG LTTK DVY FGVV+LELLSG+R  DKNRP GE+NLVEWAKPYLV K ++ ++ D R++ Q+S+  A K   L+ +CL+   KLRPNM+ VV  LE +Q
Sbjct:   31 KASSVSVR-PS-PRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGLYF--QPLSWKLRLKVALGAAKGLAFLHSSETRVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRV-MGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQ 354          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy Swiss-Prot
Match: PBL11_ARATH (Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 GN=PBL11 PE=1 SV=2)

HSP 1 Score: 413.69 bits (1062), Expect = 6.228e-136
Identity = 188/314 (59.87%), Postives = 252/314 (80.25%), Query Frame = 0

Query:  527 PPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQ 836
            P  + +IL    L+ F  +ELK+AT NF P++ +GEG FG VFKGW+D+++   +KPGT +++AVK +N+EG QGH EWL EI YLG+L HPN+VKL+GYC E++HRLLVYEFM  GSLE HLF   ++ QPLSW  R+++ALGAA+ LAF+H+++ +VI+RDFK+SNILLDSNYNAKLSDFG A+DGP GD+SHVSTRVMGT GYAAPEY+ATGHL+ K+DVY FGVV+LELLSGR+ +D ++P  E +LV+W++PYL +KRR+L++ D R++GQYS+  A K A LA  C+S++ + RP MNE+VK +E+L 
Sbjct:   41 PRTEGEILQNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKRLNQEGFQGHREWLAEINYLGQLDHPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFYQPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDVYSFGVVLLELLSGRRAIDKNQPVGEHNLVDWARPYLTNKRRLLRVMDPRLQGQYSLTRALKIAVLALDCISIDAKSRPTMNEIVKTMEELH 354          

HSP 2 Score: 399.823 bits (1026), Expect = 1.449e-130
Identity = 196/334 (58.68%), Postives = 254/334 (76.05%), Query Frame = 0

Query:  119 PQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKL----QQSSDRDDLRISQD 443
            P+T+ EIL    LK+F  +ELK+ATRNFRPD+V+GEGGFG V+KGWID++SLA S+PGTG+ +A+KRL+Q+  QG  EW  E+ YLGQL HPNLVKLIGYC E+   LLVYEF+ RGSLEN+LFR  + +  +PLSWN R+++ALGAA+ LAFLHN + Q+I+RDFK+SNILLDS YN KLSDFGL  DG    +    T+V +GT GYAAPEY+ATG L+ K DVY FGVV+LELLSG+R  DKN+P GE NLV+WA+PYL  K R+L++ D R++GQ+S+ RALK   LA  C+S + K RP MN +VK +E+L    + S ++ + +IS D
Sbjct:   41 PRTEGEILQNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKRLNQEGFQGHREWLAEINYLGQLDHPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHLFRRGTFY--QPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRV-MGTQGYAAPEYLATGHLSVKSDVYSFGVVLLELLSGRRAIDKNQPVGEHNLVDWARPYLTNKRRLLRVMDPRLQGQYSLTRALKIAVLALDCISIDAKSRPTMNEIVKTMEELHIQKEASKEQQNPQISID 371          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy Swiss-Prot
Match: BIK1_ARATH (Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=1 SV=1)

HSP 1 Score: 412.149 bits (1058), Expect = 3.733e-135
Identity = 188/310 (60.65%), Postives = 247/310 (79.68%), Query Frame = 0

Query:  529 IQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQES 838
            I S   ++ F +NELK AT NF P++ +GEG FG VFKGW+D++T T  KPGT +++AVK +N+EG QGH EWLTEI YLG+L HPN+VKL+GYC ED+HRLLVYEFM  GSLE HLF   +Y +PL W LR+ +AL AAK LAF+HS   KVI+RD K+SNILLD++YNAKLSDFG A+DGP GD S+VSTRVMGTYGYAAPEY+++GHL  ++DVY FGV++LE+LSG++ LD +RP +E++LV+W++PYL SKR+VL I D R+  QY    A + A++A +CLS EP+ RP M++VV+AL+QLQ++
Sbjct:   46 ILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKGWLDESTLTPTKPGTGLVIAVKKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEHRLLVYEFMQKGSLENHLFRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAKLSDFGLARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNARSDVYSFGVLLLEILSGKRALDHNRPAKEENLVDWARPYLTSKRKVLLIVDNRLDTQYLPEEAVRMASVAVQCLSFEPKSRPTMDQVVRALQQLQDN 355          

HSP 2 Score: 371.318 bits (952), Expect = 1.795e-119
Identity = 192/380 (50.53%), Postives = 256/380 (67.37%), Query Frame = 0

Query:   97 KSRDSNGTRIRAESLPYRVPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNE-AQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSRNGIGTMSGGIRTSVKGF 471
            KS D  G  + +      V +  +T+ EIL    +KSF +NELK ATRNFRPD+V+GEGGFG V+KGW+D+++L  ++PGTG+ +A+K+L+Q+  QG  EW  E+ YLGQL HPNLVKLIGYC ED   LLVYEF+ +GSLEN+LFR  + F  +PL W  R+ +AL AAK LAFLH++  ++I+RD K+SNILLD+ YN KLSDFGL  DG         T+V +GTYGYAAPEY+++G L  + DVY FGV++LE+LSGKR  D NRP  E+NLV+WA+PYL  K +VL I D R+  Q+    A++   +A QCLS  PK RP M+ VV+AL++LQ     D+L     KP Q         +G  +G  ++S K F
Sbjct:   18 KSSDLYGLSLSSRKSSSTVAAAQKTEGEILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKGWLDESTLTPTKPGTGLVIAVKKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEHRLLVYEFMQKGSLENHLFRRGAYF--KPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAKLSDFGLARDGPMGDLSYVSTRV-MGTYGYAAPEYMSSGHLNARSDVYSFGVLLLEILSGKRALDHNRPAKEENLVDWARPYLTSKRKVLLIVDNRLDTQYLPEEAVRMASVAVQCLSFEPKSRPTMDQVVRALQQLQ-----DNL----GKPSQTNPVKDTKKLGFKTGTTKSSEKRF 385          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy Swiss-Prot
Match: PBL1_ARATH (Probable serine/threonine-protein kinase PBL1 OS=Arabidopsis thaliana OX=3702 GN=PBL1 PE=1 SV=1)

HSP 1 Score: 397.127 bits (1019), Expect = 1.732e-129
Identity = 186/311 (59.81%), Postives = 241/311 (77.49%), Query Frame = 0

Query:  529 IQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSY-LQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQES 838
            I S   ++ F +NELK AT NF  ++ +GEG FG VF+GW+D+ T T  K  + +++AVK +N +G QGH EWLTEI YLG+L HPN+VKL+GYC ED+ RLLVYEFM  GSLE HLF   +   +PLSWILR+K+AL AAK LAF+HS   KVI+RD K+SNILLDS++NAKLSDFG A+DGP G+ S+VSTRVMGT+GYAAPEYV+TGHL  ++DVY FGVV+LELL GR+ LD +RP +EQ+LV+W++PYL S+R+VL I D R+  QY    A + A++A +CLS EP+ RP M++VV+AL QLQ+S
Sbjct:   40 ILSSTTVKSFSFNELKLATRNFRSDSVVGEGGFGCVFRGWLDETTLTPTKSSSGLVIAVKRLNPDGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEQRLLVYEFMHKGSLENHLFANGNKDFKPLSWILRIKVALDAAKGLAFLHSDPVKVIYRDIKASNILLDSDFNAKLSDFGLARDGPMGEQSYVSTRVMGTFGYAAPEYVSTGHLNARSDVYSFGVVLLELLCGRQALDHNRPAKEQNLVDWARPYLTSRRKVLLIVDTRLNSQYKPEGAVRLASIAVQCLSFEPKSRPTMDQVVRALVQLQDS 350          

HSP 2 Score: 361.303 bits (926), Expect = 1.158e-115
Identity = 181/357 (50.70%), Postives = 245/357 (68.63%), Query Frame = 0

Query:  120 QTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNE-AQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQS----SDRDDLRISQDKP---CQNAQASSRNGIGTMSGGI 464
            +T+ EIL    +KSF +NELK ATRNFR D+V+GEGGFG V++GW+D+ +L  ++  +G+ +A+KRL+ D  QG  EW  E+ YLGQL HPNLVKLIGYC ED + LLVYEF+ +GSLEN+LF  + N + +PLSW  R+K+AL AAK LAFLH++  ++I+RD K+SNILLDS +N KLSDFGL  DG         T+V +GT+GYAAPEY++TG L  + DVY FGVV+LELL G++  D NRP  EQNLV+WA+PYL  + +VL I D R+  Q+    A++   +A QCLS  PK RP M+ VV+AL +LQ S    ++ D L++   K     +      RNG+   + G+
Sbjct:   35 KTEGEILSSTTVKSFSFNELKLATRNFRSDSVVGEGGFGCVFRGWLDETTLTPTKSSSGLVIAVKRLNPDGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEQRLLVYEFMHKGSLENHLF-ANGNKDFKPLSWILRIKVALDAAKGLAFLHSDPVKVIYRDIKASNILLDSDFNAKLSDFGLARDGPMGEQSYVSTRV-MGTFGYAAPEYVSTGHLNARSDVYSFGVVLLELLCGRQALDHNRPAKEQNLVDWARPYLTSRRKVLLIVDTRLNSQYKPEGAVRLASIAVQCLSFEPKSRPTMDQVVRALVQLQDSVVKPANVDPLKVKDTKKLVGLKTEDKYQRNGLNKKTVGL 389          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy Swiss-Prot
Match: PBL3_ARATH (Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN=PBL3 PE=1 SV=1)

HSP 1 Score: 394.045 bits (1011), Expect = 8.564e-128
Identity = 187/324 (57.72%), Postives = 245/324 (75.62%), Query Frame = 0

Query:  526 TPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSG 845
            TP  + +IL    L+ F +NELK AT NF P++ LGEG FG VFKGW+D  T TA+KPG+ ++VAVK +  EG QGH+EWLTE+ YLG+L HPN+VKL+GYC E ++RLLVYEFM  GSLE HLF   +  QPL+W +RMK+A+GAAK L F+H ++ +VI+RDFK++NILLD+ +N+KLSDFG AK GP GD +HVST+VMGT+GYAAPEYVATG LT K+DVY FGVV+LELLSGR+ +D S+   EQ LV+W+ PYL  KR++ +I D R+ GQY    A  AA+LA +CL+ + +LRP M+EV+  L+QL+ +    G G
Sbjct:   55 TPRTEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGA--QPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLGGQYPQKGAYTAASLALQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVG 376          

HSP 2 Score: 370.548 bits (950), Expect = 9.724e-119
Identity = 185/332 (55.72%), Postives = 242/332 (72.89%), Query Frame = 0

Query:  109 ESLPYRVPSNPQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQS 432
            ESLP      P+T+ EIL    LK+F +NELK ATRNFRPD++LGEGGFG V+KGWID  +L AS+PG+G+ VA+K+L  +  QG +EW  EV YLGQL HPNLVKL+GYC E    LLVYEF+P+GSLEN+LFR  +    +PL+W  RMK+A+GAAK L FLH+ ++Q+I+RDFK++NILLD+ +N KLSDFGL   G +    G KT V   ++GT+GYAAPEY+ATGRLT K DVY FGVV+LELLSG+R  DK++   EQ+LV+WA PYL  K ++ +I D R+ GQ+    A  A  LA QCL+ + KLRP M+ V+  L++L+ +
Sbjct:   51 ESLP-----TPRTEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGA----QPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPT----GDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLGGQYPQKGAYTAASLALQCLNPDAKLRPKMSEVLAKLDQLEST 369          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy Swiss-Prot
Match: PBL8_ARATH (Probable serine/threonine-protein kinase PBL8 OS=Arabidopsis thaliana OX=3702 GN=PBL8 PE=1 SV=1)

HSP 1 Score: 390.578 bits (1002), Expect = 1.125e-126
Identity = 193/324 (59.57%), Postives = 243/324 (75.00%), Query Frame = 0

Query:  524 PSTPPIQSD-------ILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGN 840
            PSTP  + D         +  F   EL+T T +F P+  LGEG FG V+KG++DDN     K   S+ VAVK +N+EGLQGH EWLTE+ +LG+L HPN+VKL+GYC EDDHRLLVYEFM  GSLE HLF   +   PLSW  RM IALGAAK LAF+H++E  VI+RDFK+SNILLDS+Y AKLSDFG AK GP GD +HVSTRVMGTYGYAAPEYV TGHLT ++DVY FGVV+LE+L+GRK +D +RP++EQ+LV+W++P L  KR++L+I D R++ QYSV  A+KA +LA  CLS  P+ RP M++VV+ LE LQ +G+
Sbjct:   36 PSTPRFRDDSRTPISYAQVIPFTLFELETITKSFRPDYILGEGGFGTVYKGYIDDNLRVGLK---SLPVAVKVLNKEGLQGHREWLTEVNFLGQLRHPNLVKLIGYCCEDDHRLLVYEFMLRGSLENHLFRKTT--APLSWSRRMMIALGAAKGLAFLHNAERPVIYRDFKTSNILLDSDYTAKLSDFGLAKAGPQGDETHVSTRVMGTYGYAAPEYVMTGHLTARSDVYSFGVVLLEMLTGRKSVDKTRPSKEQNLVDWARPKLNDKRKLLQIIDPRLENQYSVRAAQKACSLAYYCLSQNPKARPLMSDVVETLEPLQCTGD 354          

HSP 2 Score: 347.436 bits (890), Expect = 4.091e-110
Identity = 176/306 (57.52%), Postives = 219/306 (71.57%), Query Frame = 0

Query:  130 FFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSD 434
            F   EL+  T++FRPD +LGEGGFG VYKG+ID N     +    + VA+K L+++ +QG  EW  EV +LGQLRHPNLVKLIGYC ED+  LLVYEF+ RGSLEN+LFR ++     PLSW+ RM IALGAAK LAFLHN E  +I+RDFK+SNILLDS Y  KLSDFGL   G         T+V+ GTYGYAAPEY+ TG LT + DVY FGVV+LE+L+G++  DK RP  EQNLV+WA+P L  K ++LQI D R+  Q+SV  A KA  LA+ CLS+NPK RP M++VV+ LE LQ + D
Sbjct:   57 FTLFELETITKSFRPDYILGEGGFGTVYKGYIDDNLRVGLK---SLPVAVKVLNKEGLQGHREWLTEVNFLGQLRHPNLVKLIGYCCEDDHRLLVYEFMLRGSLENHLFRKTT----APLSWSRRMMIALGAAKGLAFLHNAERPVIYRDFKTSNILLDSDYTAKLSDFGLAKAGPQGDETHVSTRVM-GTYGYAAPEYVMTGHLTARSDVYSFGVVLLEMLTGRKSVDKTRPSKEQNLVDWARPKLNDKRKLLQIIDPRLENQYSVRAAQKACSLAYYCLSQNPKARPLMSDVVETLEPLQCTGD 354          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy Swiss-Prot
Match: PIX7_ARATH (Probable serine/threonine-protein kinase PIX7 OS=Arabidopsis thaliana OX=3702 GN=PIX7 PE=1 SV=1)

HSP 1 Score: 393.66 bits (1010), Expect = 1.291e-126
Identity = 187/316 (59.18%), Postives = 240/316 (75.95%), Query Frame = 0

Query:  525 STPPIQSDI----LLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGK-VIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQL 835
            STP I  ++     LR+F +N+LK +T NF PE+ LGEG FG VFKGW+++N +   KPGT + VAVK +N +GLQGH+EWL EI +LG L HPN+VKL+GYC EDD RLLVYEFM  GSLE HLF       PL W +RMKIALGAAK L+F+H    K VI+RDFK+SNILLD++YNAKLSDFG AKD P    +HVSTRVMGTYGYAAPEYV TGHLT K+DVY FGVV+LE+L+GR+ +D +RP  E +LVEW++P+L  KRR  ++ D R++G +S+  A+K   LA++CLS +P++RP M++VV+AL+ L
Sbjct:  113 STPVISEELNISSHLRKFTFNDLKLSTRNFRPESLLGEGGFGCVFKGWIEENGTAPVKPGTGLTVAVKTLNPDGLQGHKEWLAEINFLGNLLHPNLVKLVGYCIEDDQRLLVYEFMPRGSLENHLF---RRSLPLPWSIRMKIALGAAKGLSFLHEEALKPVIYRDFKTSNILLDADYNAKLSDFGLAKDAPDEGKTHVSTRVMGTYGYAAPEYVMTGHLTSKSDVYSFGVVLLEMLTGRRSMDKNRPNGEHNLVEWARPHLLDKRRFYRLLDPRLEGHFSIKGAQKVTQLAAQCLSRDPKIRPKMSDVVEALKPL 425          

HSP 2 Score: 384.415 bits (986), Expect = 3.965e-123
Identity = 182/317 (57.41%), Postives = 235/317 (74.13%), Query Frame = 0

Query:  120 QTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNEA--QLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSD 434
            + ++   L+ F +N+LK +TRNFRP+++LGEGGFG V+KGWI++N  A  +PGTG+ VA+K L+ D +QG +EW  E+ +LG L HPNLVKL+GYC ED++ LLVYEF+PRGSLEN+LFR S      PL W+ RMKIALGAAK L+FLH EA   +I+RDFK+SNILLD+ YN KLSDFGL  D          T+V +GTYGYAAPEY+ TG LT+K DVY FGVV+LE+L+G+R  DKNRP GE NLVEWA+P+L+ K R  ++ D R+ G FS+  A K   LA QCLSR+PK+RP M++VV+AL+ L    D
Sbjct:  120 ELNISSHLRKFTFNDLKLSTRNFRPESLLGEGGFGCVFKGWIEENGTAPVKPGTGLTVAVKTLNPDGLQGHKEWLAEINFLGNLLHPNLVKLVGYCIEDDQRLLVYEFMPRGSLENHLFRRSL-----PLPWSIRMKIALGAAKGLSFLHEEALKPVIYRDFKTSNILLDADYNAKLSDFGLAKDAPDEGKTHVSTRV-MGTYGYAAPEYVMTGHLTSKSDVYSFGVVLLEMLTGRRSMDKNRPNGEHNLVEWARPHLLDKRRFYRLLDPRLEGHFSIKGAQKVTQLAAQCLSRDPKIRPKMSDVVEALKPLPHLKD 430          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy Swiss-Prot
Match: Y3130_ARATH (Receptor-like serine/threonine-protein kinase At3g01300 OS=Arabidopsis thaliana OX=3702 GN=At3g01300 PE=2 SV=1)

HSP 1 Score: 386.341 bits (991), Expect = 6.428e-124
Identity = 188/316 (59.49%), Postives = 235/316 (74.37%), Query Frame = 0

Query:  525 STPPIQSDI----LLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGK-VIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQL 835
            STP I  ++     L++F + +LK AT NF PE+ LGEG FG VFKGWV++N +   KPGT + VAVK +N +GLQGH+EWL EI YLG L HPN+VKL+GYC EDD RLLVYEFM  GSLE HLF       PL W +RMKIALGAAK L+F+H    K VI+RDFK+SNILLD  YNAKLSDFG AKD P    +HVSTRVMGTYGYAAPEYV TGHLT K+DVY FGVV+LE+L+GR+ +D +RP  E +LVEW++P+L  KRR  ++ D R++G +SV  A+K   LA++CLS + ++RP M+EVV+ L+ L
Sbjct:  107 STPIISEELNIYSHLKKFSFIDLKLATRNFRPESLLGEGGFGCVFKGWVEENGTAPVKPGTGLTVAVKTLNPDGLQGHKEWLAEINYLGNLLHPNLVKLVGYCIEDDQRLLVYEFMPRGSLENHLF---RRSLPLPWSIRMKIALGAAKGLSFLHEEALKPVIYRDFKTSNILLDGEYNAKLSDFGLAKDAPDEGKTHVSTRVMGTYGYAAPEYVMTGHLTSKSDVYSFGVVLLEMLTGRRSMDKNRPNGEHNLVEWARPHLLDKRRFYRLLDPRLEGHFSVKGAQKVTQLAAQCLSRDSKIRPKMSEVVEVLKPL 419          

HSP 2 Score: 377.867 bits (969), Expect = 1.102e-120
Identity = 182/310 (58.71%), Postives = 227/310 (73.23%), Query Frame = 0

Query:  127 LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNEA--QLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSD 434
            LK F + +LK ATRNFRP+++LGEGGFG V+KGW+++N  A  +PGTG+ VA+K L+ D +QG +EW  E+ YLG L HPNLVKL+GYC ED++ LLVYEF+PRGSLEN+LFR S      PL W+ RMKIALGAAK L+FLH EA   +I+RDFK+SNILLD  YN KLSDFGL  D          T+V +GTYGYAAPEY+ TG LT+K DVY FGVV+LE+L+G+R  DKNRP GE NLVEWA+P+L+ K R  ++ D R+ G FSV  A K   LA QCLSR+ K+RP M+ VV+ L+ L    D
Sbjct:  121 LKKFSFIDLKLATRNFRPESLLGEGGFGCVFKGWVEENGTAPVKPGTGLTVAVKTLNPDGLQGHKEWLAEINYLGNLLHPNLVKLVGYCIEDDQRLLVYEFMPRGSLENHLFRRSL-----PLPWSIRMKIALGAAKGLSFLHEEALKPVIYRDFKTSNILLDGEYNAKLSDFGLAKDAPDEGKTHVSTRV-MGTYGYAAPEYVMTGHLTSKSDVYSFGVVLLEMLTGRRSMDKNRPNGEHNLVEWARPHLLDKRRFYRLLDPRLEGHFSVKGAQKVTQLAAQCLSRDSKIRPKMSEVVEVLKPLPHLKD 424          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. NCBI nr
Match: gi|1358184708|gb|PRQ59182.1| (putative transferase, protein kinase RLK-Pelle-RLCK-VIIa-2 family [Rosa chinensis])

HSP 1 Score: 1074.69 bits (2778), Expect = 0.000e+0
Identity = 554/833 (66.51%), Postives = 641/833 (76.95%), Query Frame = 0

Query:  107 RAESLPY----RVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSRNGI-----GTMSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAAADSVDKMKAQEKS--------------------------MFSICD----------------------------VPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISF 872
            R  SLP      V S P+T+ EILL+ FF NELK AT NF PD ++GEGGFG V+KGW+D +S AA+ PG GM +A+KR++++   G E+W  E+YYLG+LRHPNLVKL+GYC ED   LLV+EF+ RG L+N++F   S   ++PLSWN RMKI+L AA  LAFLHN E  ++ RD  SSNILLDSTYN KL+DFGL  DG +    G K+ V   ++GT+GYAAPEYIATG LT KCDVYGFGVVMLE+LSG+R  D++ P  +QNLV+WAKPYL  K RVL++FD  ++GQ+SVA ALKA +LA QCLS  P+ RPNM  VVKALE+LQ   D   L+I Q++PCQ+ +A+S N        TMSGGIRTSVKGFLNAKRLA S+EIDTVHETNE IT NF+ALQAAADSVDK KAQEKS                          +F IC                             +PS+P  Q++IL RRFFYNELKTATWNFHP N +GEG +G+VFKGW+DDNTST+AKPG+ ML+AVK I  EGL+G +EWLTEIYYLGRL HPNVVKLLGYCF+DDHRLLVYEFMA G+LE  L GWVSYLQPLSWILRMKIALGAAK+LAFIH+SEGKVIHRDFKSSNILLDSNYNAKL+DFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLT KNDVYG+GVVMLELL+G+ VLD SRPTREQDLVEW+KPYL SKRRVLKI DARIKGQYSVA ARKAANL ++CLS EP LRPNMNEVVK LEQLQESG+IEG+GIS +E  QS YSNS+S KSSW+SWISF
Sbjct:    4 RKHSLPAVSSAAVSSCPRTEGEILLRRFFLNELKMATWNFHPDNMVGEGGFGSVFKGWVDDSS-AAANPGKGMLIAVKRINKEGFLGHEQWLAEIYYLGRLRHPNLVKLLGYCFEDGHRLLVFEFMSRGGLDNHIFGRDSC--LQPLSWNLRMKISLEAANVLAFLHNGEETVVHRDITSSNILLDSTYNAKLADFGLAKDGPT----GDKSHVSTAVMGTHGYAAPEYIATGHLTAKCDVYGFGVVMLEMLSGRRSLDQSLPTVQQNLVDWAKPYLASKRRVLKVFDPCLKGQYSVAGALKAANLAKQCLSEEPEFRPNMTEVVKALEQLQGPEDMKGLKIPQNEPCQSPRANSSNRRRSTRRATMSGGIRTSVKGFLNAKRLALSAEIDTVHETNETITLNFSALQAAADSVDKKKAQEKSIASTSYGLSIYDGSFPMFNSAPKLFFLFLICITNLNIVSCTNMLQFAYLNSFCCAIEGSMLPSSPRTQNEILPRRFFYNELKTATWNFHPVNMVGEGCYGRVFKGWIDDNTSTSAKPGSGMLIAVKTIKGEGLRGQKEWLTEIYYLGRLRHPNVVKLLGYCFDDDHRLLVYEFMAKGNLEFRLVGWVSYLQPLSWILRMKIALGAAKALAFIHNSEGKVIHRDFKSSNILLDSNYNAKLADFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTSKNDVYGYGVVMLELLTGKPVLDTSRPTREQDLVEWAKPYLDSKRRVLKILDARIKGQYSVAAARKAANLPNQCLSAEPELRPNMNEVVKELEQLQESGDIEGAGISHSEPCQSPYSNSSSVKSSWRSWISF 829          

HSP 2 Score: 436.032 bits (1120), Expect = 8.928e-136
Identity = 248/505 (49.11%), Postives = 321/505 (63.56%), Query Frame = 0

Query:    1 MNDVVKALEELQESE--------------------------FSIEDMGGGIRTSVKGYLLSQRITMAS--DTVHE-NESINRNFTAPLAA----DTEQETMMDLDSTNSFRFSRFSGG-----------------------IRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQN 448
            M +VVKALE+LQ  E                               M GGIRTSVKG+L ++R+ +++  DTVHE NE+I  NF+A  AA    D ++     + ST S+  S + G                        + C  +L FA    +S    I    L    PS+P+T  EIL + FFYNELK AT NF P  ++GEG +G V+KGWID N+  +++PG+GM +A+K +  + ++G++EW  E+YYLG+LRHPN+VKL+GYC +D+  LLVYEF+ +G+LE  L    S   ++PLSW  RMKIALGAAKALAF+HN E ++I RDFKSSNILLDS YN KL+DFG   DG +       T+V +GTYGYAAPEY+ATG LT+K DVYG+GVVMLELL+GK V D +RP  EQ+LVEWAKPYL  K RVL+I DARI+GQ+SVA A KA +L  QCLS  P+LRPNMN VVK LE+LQ+S D +   IS  +PCQ+
Sbjct:  317 MTEVVKALEQLQGPEDMKGLKIPQNEPCQSPRANSSNRRRSTRRATMSGGIRTSVKGFLNAKRLALSAEIDTVHETNETITLNFSALQAAADSVDKKKAQEKSIAST-SYGLSIYDGSFPMFNSAPKLFFLFLICITNLNIVSCTNMLQFA--YLNSFCCAIEGSML----PSSPRTQNEILPRRFFYNELKTATWNFHPVNMVGEGCYGRVFKGWIDDNTSTSAKPGSGMLIAVKTIKGEGLRGQKEWLTEIYYLGRLRHPNVVKLLGYCFDDDHRLLVYEFMAKGNLEFRLVGWVS--YLQPLSWILRMKIALGAAKALAFIHNSEGKVIHRDFKSSNILLDSNYNAKLADFGFAKDGPAGDSSHVSTRV-MGTYGYAAPEYVATGHLTSKNDVYGYGVVMLELLTGKPVLDTSRPTREQDLVEWAKPYLDSKRRVLKILDARIKGQYSVAAARKAANLPNQCLSAEPELRPNMNEVVKELEQLQESGDIEGAGISHSEPCQS 811          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. NCBI nr
Match: gi|470146484|ref|XP_004308854.1| (PREDICTED: protein kinase APK1B, chloroplastic-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 857.44 bits (2214), Expect = 0.000e+0
Identity = 412/436 (94.50%), Postives = 422/436 (96.79%), Query Frame = 0

Query:   21 MGGGIRTSVKGYLLSQRITMASDTVHENESINRNFTAPLAADTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSRNG 456
            MGGGIRTSVKGYLLSQRITMASDTVHENES NRNFTA LAADTE++T MDLDSTNS+RFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNP+TDVEILLKSFFYNELKAATRNFRPDT+LGEGGFGPVYKGWIDQN LAASEPGTGMAVAIKRLDQDSMQGREEW KEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLEN+LFRGSSNFEVEPLSWNARMKIALGAAKAL FLHNEA LIFRDFK+SNILLDSTYNVKLSDFGL VDGRSIRHPGAKTKVI+GTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGK VFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARI+GQFSVA ALKAVDLAF CLSRNPKLRPNM NVVKALEKLQQS + DDLRISQDKPCQNAQASSRNG
Sbjct:    1 MGGGIRTSVKGYLLSQRITMASDTVHENESTNRNFTASLAADTERKTRMDLDSTNSYRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPRTDVEILLKSFFYNELKAATRNFRPDTLLGEGGFGPVYKGWIDQNPLAASEPGTGMAVAIKRLDQDSMQGREEWSKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENHLFRGSSNFEVEPLSWNARMKIALGAAKALTFLHNEALLIFRDFKTSNILLDSTYNVKLSDFGLAVDGRSIRHPGAKTKVIMGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKLVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIKGQFSVASALKAVDLAFLCLSRNPKLRPNMKNVVKALEKLQQSCNMDDLRISQDKPCQNAQASSRNG 436          

HSP 2 Score: 436.032 bits (1120), Expect = 2.218e-141
Identity = 240/438 (54.79%), Postives = 296/438 (67.58%), Query Frame = 0

Query:  460 MSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAAADS-------VDKMKAQEKSMFS---IC-------------------------DVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVS--YLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRV-MGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNS 859
            M GGIRTSVKG+L ++R+  +S  DTVHE NE+  RNF A   AAD+       +D   +   S FS    C                          VPS P    +ILL+ FFYNELK AT NF P+  LGEG FG V+KGW+D N   A++PGT M VA+K ++++ +QG EEW  E+YYLG+L HPN+VKL+GYC ED+  LLVYEF+  GSLE HLF   S   ++PLSW  RMKIALGAAK+L F+H+ E  +I RDFK+SNILLDS YN KLSDFG A DG +       T+V MGTYGYAAPEY+ATG LT K DVYGFGVVMLELLSG+ V D +RP  EQ+LVEW+KPYL  K RVL+IFDARIKGQ+SVA+A KA +LA  CLS  P+LRPNM  VVKALE+LQ+S N++   IS ++  Q+  ++S
Sbjct:    1 MGGGIRTSVKGYLLSQRITMAS--DTVHE-NESTNRNFTA-SLAADTERKTRMDLDSTNSYRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPRTDVEILLKSFFYNELKAATRNFRPDTLLGEGGFGPVYKGWIDQNPLAASEPGTGMAVAIKRLDQDSMQGREEWSKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENHLFRGSSNFEVEPLSWNARMKIALGAAKALTFLHN-EALLIFRDFKTSNILLDSTYNVKLSDFGLAVDGRSIRHPGAKTKVIMGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKLVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIKGQFSVASALKAVDLAFLCLSRNPKLRPNMKNVVKALEKLQQSCNMDDLRISQDKPCQNAQASS 433          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. NCBI nr
Match: gi|764632484|ref|XP_004307505.2| (PREDICTED: protein kinase APK1B, chloroplastic-like isoform X1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 820.076 bits (2117), Expect = 0.000e+0
Identity = 406/432 (93.98%), Postives = 413/432 (95.60%), Query Frame = 0

Query:  455 NGIGTMSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENI----TRNFAALQAAADSVDKMKAQEKSMFSICDVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYIR 882
            +G GTMSGGIRTSVKGFLNAKRLA SS+IDTVHETNENI    TRNF ALQAAADSVDKMKAQE  MFSICDVPSTP  Q + LLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGT MLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDH+LLVYEFMAY SLE+HLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAG SSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVL+ISRPTREQDLVEW+KPYLASKR VLKIFDARIKGQYSVA ARKAANLASRCLSVEPRLRPNMNEVV ALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYIR
Sbjct:    4 SGTGTMSGGIRTSVKGFLNAKRLALSSKIDTVHETNENINENITRNFTALQAAADSVDKMKAQETPMFSICDVPSTPQTQGENLLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTGMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHQLLVYEFMAYRSLEVHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGGSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLEISRPTREQDLVEWAKPYLASKRGVLKIFDARIKGQYSVAAARKAANLASRCLSVEPRLRPNMNEVVTALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYIR 435          

HSP 2 Score: 427.557 bits (1098), Expect = 4.302e-138
Identity = 237/441 (53.74%), Postives = 299/441 (67.80%), Query Frame = 0

Query:   21 MGGGIRTSVKGYLLSQRITMAS--DTVHE-----NESINRNFTAPLAADTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASS 453
            M GGIRTSVKG+L ++R+ ++S  DTVHE     NE+I RNFTA  AA    + M   ++                P+ S                     VPS PQT  E LL+ FFYNELK AT NF P+  LGEG FG V+KGW+D N+  A++PGTGM VA+K ++++ +QG EEW  E+YYLG+L HPN+VKL+GYC ED+  LLVYEF+   SLE +LF   S   ++PLSW  RMKIALGAAK+LAF+H+ E ++I RDFKSSNILLDS YN KLSDFG   DG +       T+V+ GTYGYAAPEY+ATG LT K DVYGFGVVMLELLSG++V + +RP  EQ+LVEWAKPYL  K  VL+IFDARI+GQ+SVA A KA +LA +CLS  P+LRPNMN VV ALE+LQ+S + +   IS ++  Q+  ++S
Sbjct:    9 MSGGIRTSVKGFLNAKRLALSSKIDTVHETNENINENITRNFTALQAAADSVDKMKAQET----------------PMFSICD------------------VPSTPQTQGENLLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTGMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHQLLVYEFMAYRSLEVHLFGWVS--YLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGGSSHVSTRVM-GTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLEISRPTREQDLVEWAKPYLASKRGVLKIFDARIKGQYSVAAARKAANLASRCLSVEPRLRPNMNEVVTALEQLQESGNIEGSGISLNELRQSLYSNS 412          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. NCBI nr
Match: gi|470146488|ref|XP_004308856.1| (PREDICTED: probable serine/threonine-protein kinase NAK [Fragaria vesca subsp. vesca] >gi|764632516|ref|XP_011469769.1| PREDICTED: probable serine/threonine-protein kinase NAK [Fragaria vesca subsp. vesca] >gi|764632519|ref|XP_011469770.1| PREDICTED: probable serine/threonine-protein kinase NAK [Fragaria vesca subsp. vesca])

HSP 1 Score: 804.668 bits (2077), Expect = 0.000e+0
Identity = 395/454 (87.00%), Postives = 411/454 (90.53%), Query Frame = 0

Query:   21 MGGGIRTSVKGYLLSQRITMASDTVHENESINRNFTAPLAA---DTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---------------IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSRNG 456
            MGGGIRTSVKGYLLSQRITMASDTVHENES NR+FTAPLAA   DTEQET MDLD+TNS+RF RFSG IRCLPILSFASKSR SNGT IRAESLPYRVPSNP+TDVEILLKSFF+ ELK ATRNF PDTV+GEGGFGPV+KGWIDQNSLAASEPGTGMAVAIKRL+Q+SMQGREEW KEVYYLGQLRHPNLVKLIGYCSEDNELLLVYE+VPRGSLEN LFR SSN  VEPLSWNARMKIALGAAKAL FLHNEAQLIFRDFKSSNILLDSTYNVKL+DFGLV+DGRSIRHPGAKTKV               ++GTYGYAAPEYIATGRLTTKCDVYGFG VMLELLSGKRVFDKNRPPGEQNLVEWAKP LVRKHRVLQIFD  I+GQFSV  +LKAVDLAFQCLSRNPKLRPNM NVVKALEKLQQS D DDLRISQDKPCQNAQASS NG
Sbjct:    1 MGGGIRTSVKGYLLSQRITMASDTVHENESSNRSFTAPLAAETADTEQETRMDLDTTNSYRFGRFSGVIRCLPILSFASKSRVSNGTSIRAESLPYRVPSNPRTDVEILLKSFFFKELKTATRNFGPDTVVGEGGFGPVFKGWIDQNSLAASEPGTGMAVAIKRLNQNSMQGREEWSKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEYVPRGSLENNLFRRSSNL-VEPLSWNARMKIALGAAKALTFLHNEAQLIFRDFKSSNILLDSTYNVKLADFGLVMDGRSIRHPGAKTKVMGTYGYAAPEYLETRVLGTYGYAAPEYIATGRLTTKCDVYGFGAVMLELLSGKRVFDKNRPPGEQNLVEWAKPLLVRKHRVLQIFDGYIKGQFSVTSSLKAVDLAFQCLSRNPKLRPNMKNVVKALEKLQQSCDMDDLRISQDKPCQNAQASSSNG 453          

HSP 2 Score: 416.772 bits (1070), Expect = 1.114e-133
Identity = 231/457 (50.55%), Postives = 298/457 (65.21%), Query Frame = 0

Query:  460 MSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAA--ADS-------VDKMKAQEKSMFS---IC-------------------------DVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYL-QPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDG-----PAGDSS-----------HVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSA 862
            M GGIRTSVKG+L ++R+  +S  DTVHE NE+  R+F A  AA  AD+       +D   +     FS    C                          VPS P    +ILL+ FF+ ELKTAT NF P+  +GEG FG VFKGW+D N+  A++PGT M VA+K +N+  +QG EEW  E+YYLG+L HPN+VKL+GYC ED+  LLVYE++  GSLE +LF   S L +PLSW  RMKIALGAAK+L F+H+ E ++I RDFKSSNILLDS YN KL+DFG   DG     P   +            ++ TRV+GTYGYAAPEY+ATG LT K DVYGFG VMLELLSG++V D +RP  EQ+LVEW+KP L  K RVL+IFD  IKGQ+SV ++ KA +LA +CLS  P+LRPNM  VVKALE+LQ+S +++   IS ++  Q+  ++S++ 
Sbjct:    1 MGGGIRTSVKGYLLSQRITMAS--DTVHE-NESSNRSFTAPLAAETADTEQETRMDLDTTNSYRFGRFSGVIRCLPILSFASKSRVSNGTSIRAESLPYRVPSNPRTDVEILLKSFFFKELKTATRNFGPDTVVGEGGFGPVFKGWIDQNSLAASEPGTGMAVAIKRLNQNSMQGREEWSKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEYVPRGSLENNLFRRSSNLVEPLSWNARMKIALGAAKALTFLHN-EAQLIFRDFKSSNILLDSTYNVKLADFGLVMDGRSIRHPGAKTKVMGTYGYAAPEYLETRVLGTYGYAAPEYIATGRLTTKCDVYGFGAVMLELLSGKRVFDKNRPPGEQNLVEWAKPLLVRKHRVLQIFDGYIKGQFSVTSSLKAVDLAFQCLSRNPKLRPNMKNVVKALEKLQQSCDMDDLRISQDKPCQNAQASSSNG 453          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. NCBI nr
Match: gi|764632487|ref|XP_011469764.1| (PREDICTED: protein kinase APK1B, chloroplastic-like isoform X2 [Fragaria vesca subsp. vesca] >gi|764632491|ref|XP_011469765.1| PREDICTED: protein kinase APK1B, chloroplastic-like isoform X2 [Fragaria vesca subsp. vesca])

HSP 1 Score: 803.512 bits (2074), Expect = 0.000e+0
Identity = 402/434 (92.63%), Postives = 409/434 (94.24%), Query Frame = 0

Query:  460 MSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENI----TRNFAALQAAADSVDKMKAQEKSMFSICDVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWV-------SYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYIR 882
            MSGGIRTSVKGFLNAKRLA SS+IDTVHETNENI    TRNF ALQAAADSVDKMKAQE  MFSICDVPSTP  Q + LLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGT MLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDH+LLVYEFMAY SLE+HLFG+V       SYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAG SSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVL+ISRPTREQDLVEW+KPYLASKR VLKIFDARIKGQYSVA ARKAANLASRCLSVEPRLRPNMNEVV ALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYIR
Sbjct:    1 MSGGIRTSVKGFLNAKRLALSSKIDTVHETNENINENITRNFTALQAAADSVDKMKAQETPMFSICDVPSTPQTQGENLLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTGMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHQLLVYEFMAYRSLEVHLFGYVVGLGTGVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGGSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLEISRPTREQDLVEWAKPYLASKRGVLKIFDARIKGQYSVAAARKAANLASRCLSVEPRLRPNMNEVVTALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYIR 434          

HSP 2 Score: 426.787 bits (1096), Expect = 7.830e-138
Identity = 237/446 (53.14%), Postives = 300/446 (67.26%), Query Frame = 0

Query:   21 MGGGIRTSVKGYLLSQRITMAS--DTVHE-----NESINRNFTAPLAADTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLF-----RGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASS 453
            M GGIRTSVKG+L ++R+ ++S  DTVHE     NE+I RNFTA  AA    + M   ++                P+ S                     VPS PQT  E LL+ FFYNELK AT NF P+  LGEG FG V+KGW+D N+  A++PGTGM VA+K ++++ +QG EEW  E+YYLG+L HPN+VKL+GYC ED+  LLVYEF+   SLE +LF      G+    ++PLSW  RMKIALGAAK+LAF+H+ E ++I RDFKSSNILLDS YN KLSDFG   DG +       T+V+ GTYGYAAPEY+ATG LT K DVYGFGVVMLELLSG++V + +RP  EQ+LVEWAKPYL  K  VL+IFDARI+GQ+SVA A KA +LA +CLS  P+LRPNMN VV ALE+LQ+S + +   IS ++  Q+  ++S
Sbjct:    1 MSGGIRTSVKGFLNAKRLALSSKIDTVHETNENINENITRNFTALQAAADSVDKMKAQET----------------PMFSICD------------------VPSTPQTQGENLLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTGMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHQLLVYEFMAYRSLEVHLFGYVVGLGTGVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGGSSHVSTRVM-GTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLEISRPTREQDLVEWAKPYLASKRGVLKIFDARIKGQYSVAAARKAANLASRCLSVEPRLRPNMNEVVTALEQLQESGNIEGSGISLNELRQSLYSNS 411          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. NCBI nr
Match: gi|1358184713|gb|PRQ59187.1| (putative transferase, protein kinase RLK-Pelle-RLCK-VIIa-2 family [Rosa chinensis])

HSP 1 Score: 712.605 bits (1838), Expect = 0.000e+0
Identity = 350/449 (77.95%), Postives = 387/449 (86.19%), Query Frame = 0

Query:   20 DMGGGIRTSVKGYLLSQRITMASDTVHENESINRNFTAPLAADTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSI-RHPGAKTKVI---------------IGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQAS 452
            DMGGGIRTSVKGYLLSQRIT ASDTVH NES NRNFTAP AAD+EQET  DLDS+NS+  SRFSG I CLPIL  ASKS+ SNGTRIRAESL +RVPS P+T+VEILLKSFF+NELK ATRNFRPD V+GEGGFG V+KGWIDQNS AA++PG GMAVAIKRL+Q+S QGREEWF+E+YYLGQLRHPNLVKLIGYCSEDNELLLVYEFVP GSLEN++FR SS+F  +PLSW  RMKIALGAAKALAFLHNEA++I+RDFK++NILLDSTYNVKLSDFGL +DGR I RHPGAKT+VI               +GTYGYAAPEYIATGRL TKCDVYGFG VMLELLSG+RVFD++R PGEQ LV+WAKPYLV K RVLQIFD+ I+GQFSVARALKAVDLAF CLSR+PKLRPNMNNVVK LEKLQ+SSD D L ISQ+K  QN   S
Sbjct:   47 DMGGGIRTSVKGYLLSQRITTASDTVHANESTNRNFTAPQAADSEQETSTDLDSSNSYWLSRFSGSIPCLPILGSASKSKVSNGTRIRAESLRHRVPSTPRTEVEILLKSFFFNELKTATRNFRPDNVVGEGGFGTVFKGWIDQNSFAAAKPGKGMAVAIKRLNQESYQGREEWFREIYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPHGSLENHIFRRSSHF--QPLSWTFRMKIALGAAKALAFLHNEAKVIYRDFKAANILLDSTYNVKLSDFGLAMDGRLIDRHPGAKTRVIGTYGCAAPKYIETRVMGTYGYAAPEYIATGRLNTKCDVYGFGAVMLELLSGRRVFDRSRLPGEQKLVKWAKPYLVSKQRVLQIFDSHIKGQFSVARALKAVDLAFLCLSRDPKLRPNMNNVVKTLEKLQESSDMDGLGISQNKRRQNPHVS 493          

HSP 2 Score: 466.077 bits (1198), Expect = 3.821e-152
Identity = 249/446 (55.83%), Postives = 303/446 (67.94%), Query Frame = 0

Query:  460 MSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAA------ADSVDKMKAQEKSMFS---IC-------------------------DVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDG------PAGDS-----------SHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQS 854
            M GGIRTSVKG+L ++R+  +S  DTVH  NE+  RNF A QAA      +  +D   +   S FS    C                          VPSTP  + +ILL+ FF+NELKTAT NF P+N +GEG FG VFKGW+D N+  AAKPG  M VA+K +N+E  QG EEW  EIYYLG+L HPN+VKL+GYC ED+  LLVYEF+ +GSLE H+F   S+ QPLSW  RMKIALGAAK+LAF+H +E KVI+RDFK++NILLDS YN KLSDFG A DG      P   +            ++ TRVMGTYGYAAPEY+ATG L  K DVYGFG VMLELLSGR+V D SR   EQ LV+W+KPYL SK+RVL+IFD+ IKGQ+SVA A KA +LA  CLS +P+LRPNMN VVK LE+LQES +++G GIS N+ RQ+
Sbjct:   48 MGGGIRTSVKGYLLSQRITTAS--DTVH-ANESTNRNFTAPQAADSEQETSTDLDSSNSYWLSRFSGSIPCLPILGSASKSKVSNGTRIRAESLRHRVPSTPRTEVEILLKSFFFNELKTATRNFRPDNVVGEGGFGTVFKGWIDQNSFAAAKPGKGMAVAIKRLNQESYQGREEWFREIYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPHGSLENHIFRRSSHFQPLSWTFRMKIALGAAKALAFLH-NEAKVIYRDFKAANILLDSTYNVKLSDFGLAMDGRLIDRHPGAKTRVIGTYGCAAPKYIETRVMGTYGYAAPEYIATGRLNTKCDVYGFGAVMLELLSGRRVFDRSRLPGEQKLVKWAKPYLVSKQRVLQIFDSHIKGQFSVARALKAVDLAFLCLSRDPKLRPNMNNVVKTLEKLQESSDMDGLGISQNKRRQN 489          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. NCBI nr
Match: gi|1365953016|ref|XP_024177016.1| (receptor-like cytoplasmic kinase 176 [Rosa chinensis])

HSP 1 Score: 701.819 bits (1810), Expect = 0.000e+0
Identity = 344/448 (76.79%), Postives = 386/448 (86.16%), Query Frame = 0

Query:   21 MGGGIRTSVKGYLLSQRITMASDTVHENESINRNFTAPLAADTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSI-RHPGAKTKVI---------------IGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQAS 452
            MGGGIRTSVKGYLLSQRIT ASDTVH NES NRNFTAP AAD+EQET  DLDS+NS+  SRFSG + CLPIL  A KS+ SNGTRIRAESL +RVPS P+T+VEILL+SFF+NELK ATRNFRPD V+GEGGFG V+KGWIDQNSLAA++PG GMAVAIKRL+Q+S QGREEWF+E+YYLGQLRHPNLVKLIGYCSEDNELLLVYEFVP GSL+N++FR SS+F  +PLSW  RMKIALGAAKALAFLHNEA++I+RDFK++NILLDSTYNVKLSDFGL +DGR I RHPGAKT+VI               +GTYG+AAPEYIATGRL TKCDVYGFG VMLELLSG+RVFD++R PGEQ LV+WAKPYLV K RVLQIFD+ I+GQFSVARALKAVD+AF CLSR+PKLRPNMNNVVK LEKLQ+SSD D L ISQ+K  QN   S
Sbjct:    1 MGGGIRTSVKGYLLSQRITTASDTVHANESTNRNFTAPQAADSEQETSTDLDSSNSYWLSRFSGSMSCLPILGSAPKSKVSNGTRIRAESLRHRVPSTPRTEVEILLRSFFFNELKTATRNFRPDNVVGEGGFGTVFKGWIDQNSLAAAKPGKGMAVAIKRLNQESYQGREEWFREIYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPHGSLDNHIFRRSSHF--QPLSWTFRMKIALGAAKALAFLHNEAKVIYRDFKAANILLDSTYNVKLSDFGLAMDGRLIDRHPGAKTRVIGTYGCAAPKYIETRVMGTYGHAAPEYIATGRLNTKCDVYGFGAVMLELLSGRRVFDRSRLPGEQKLVKWAKPYLVSKQRVLQIFDSHIKGQFSVARALKAVDVAFLCLSRDPKLRPNMNNVVKTLEKLQESSDIDGLGISQNKRRQNPHVS 446          

HSP 2 Score: 463.381 bits (1191), Expect = 9.357e-152
Identity = 248/446 (55.61%), Postives = 303/446 (67.94%), Query Frame = 0

Query:  460 MSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAA------ADSVDKMKAQEKSMFS---IC-------------------------DVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDG------PAGDS-----------SHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQS 854
            M GGIRTSVKG+L ++R+  +S  DTVH  NE+  RNF A QAA      +  +D   +   S FS    C                          VPSTP  + +ILLR FF+NELKTAT NF P+N +GEG FG VFKGW+D N+  AAKPG  M VA+K +N+E  QG EEW  EIYYLG+L HPN+VKL+GYC ED+  LLVYEF+ +GSL+ H+F   S+ QPLSW  RMKIALGAAK+LAF+H +E KVI+RDFK++NILLDS YN KLSDFG A DG      P   +            ++ TRVMGTYG+AAPEY+ATG L  K DVYGFG VMLELLSGR+V D SR   EQ LV+W+KPYL SK+RVL+IFD+ IKGQ+SVA A KA ++A  CLS +P+LRPNMN VVK LE+LQES +I+G GIS N+ RQ+
Sbjct:    1 MGGGIRTSVKGYLLSQRITTAS--DTVH-ANESTNRNFTAPQAADSEQETSTDLDSSNSYWLSRFSGSMSCLPILGSAPKSKVSNGTRIRAESLRHRVPSTPRTEVEILLRSFFFNELKTATRNFRPDNVVGEGGFGTVFKGWIDQNSLAAAKPGKGMAVAIKRLNQESYQGREEWFREIYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPHGSLDNHIFRRSSHFQPLSWTFRMKIALGAAKALAFLH-NEAKVIYRDFKAANILLDSTYNVKLSDFGLAMDGRLIDRHPGAKTRVIGTYGCAAPKYIETRVMGTYGHAAPEYIATGRLNTKCDVYGFGAVMLELLSGRRVFDRSRLPGEQKLVKWAKPYLVSKQRVLQIFDSHIKGQFSVARALKAVDVAFLCLSRDPKLRPNMNNVVKTLEKLQESSDIDGLGISQNKRRQN 442          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. NCBI nr
Match: gi|1366012341|ref|XP_024159101.1| (receptor-like cytoplasmic kinase 176 isoform X3 [Rosa chinensis])

HSP 1 Score: 683.33 bits (1762), Expect = 0.000e+0
Identity = 336/420 (80.00%), Postives = 362/420 (86.19%), Query Frame = 0

Query:  460 MSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAAADSVDKMKAQEKSM------FSICDVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFR 873
            MSGG+R+SVK +LNAKRL FS EIDTV+ETNE I RNF+ALQAA DSVDK K QEKS+       S+ DVPST   +  ILL+RF +NELK ATWNFHP NKLGEG FG VF+GWVDDN STAA PGT ML+AVK IN+EGLQGH+EWLTEIYYLGRL HPN+V LLGYCFEDD RLLVYEFMA GSL+ HL+G  S LQPLSWILRMKIALGAA SLAFIHS EGKVIHRD KSSNILLDSNYNAKLSDFGFAKDGPAGD SHVST VMGT GYAAPEYVATGHLT K DVYGFGVVMLELL+G+ VLD SRPTRE++LVEW++PY ASKRRVLKIFDARIKGQYSVA ARKAANL ++CLS EP LRPNMNEVVKALEQLQESGN EGSGIS  E RQS YSNSNSAKSSW+SWISFR
Sbjct:    1 MSGGLRSSVKSYLNAKRLPFSGEIDTVNETNETINRNFSALQAAVDSVDKKKPQEKSIASTSYQLSMYDVPSTSR-RERILLKRFLFNELKVATWNFHPHNKLGEGGFGSVFRGWVDDNISTAASPGTGMLIAVKRINQEGLQGHKEWLTEIYYLGRLRHPNLVTLLGYCFEDDCRLLVYEFMAGGSLDTHLYGRASSLQPLSWILRMKIALGAANSLAFIHSGEGKVIHRDVKSSNILLDSNYNAKLSDFGFAKDGPAGDKSHVSTWVMGTRGYAAPEYVATGHLTRKTDVYGFGVVMLELLTGKPVLDTSRPTRERNLVEWARPYFASKRRVLKIFDARIKGQYSVAAARKAANLTNQCLSTEPELRPNMNEVVKALEQLQESGNNEGSGISHKEPRQSPYSNSNSAKSSWRSWISFR 419          

HSP 2 Score: 426.402 bits (1095), Expect = 6.860e-138
Identity = 234/445 (52.58%), Postives = 299/445 (67.19%), Query Frame = 0

Query:   21 MGGGIRTSVKGYLLSQRITMAS--DTVHE-NESINRNFTAPLAA----DTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLF-RGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASS 453
            M GG+R+SVK YL ++R+  +   DTV+E NE+INRNF+A  AA    D ++     + ST S++ S                                Y VPS  + +  ILLK F +NELK AT NF P   LGEGGFG V++GW+D N   A+ PGTGM +A+KR++Q+ +QG +EW  E+YYLG+LRHPNLV L+GYC ED+  LLVYEF+  GSL+ +L+ R SS   ++PLSW  RMKIALGAA +LAF+H+ E ++I RD KSSNILLDS YN KLSDFG   DG +    G K+ V   ++GT GYAAPEY+ATG LT K DVYGFGVVMLELL+GK V D +RP  E+NLVEWA+PY   K RVL+IFDARI+GQ+SVA A KA +L  QCLS  P+LRPNMN VVKALE+LQ+S + +   IS  +P Q+  ++S
Sbjct:    1 MSGGLRSSVKSYLNAKRLPFSGEIDTVNETNETINRNFSALQAAVDSVDKKKPQEKSIAST-SYQLSM-------------------------------YDVPSTSRRE-RILLKRFLFNELKVATWNFHPHNKLGEGGFGSVFRGWVDDNISTAASPGTGMLIAVKRINQEGLQGHKEWLTEIYYLGRLRHPNLVTLLGYCFEDDCRLLVYEFMAGGSLDTHLYGRASS---LQPLSWILRMKIALGAANSLAFIHSGEGKVIHRDVKSSNILLDSNYNAKLSDFGFAKDGPA----GDKSHVSTWVMGTRGYAAPEYVATGHLTRKTDVYGFGVVMLELLTGKPVLDTSRPTRERNLVEWARPYFASKRRVLKIFDARIKGQYSVAAARKAANLTNQCLSTEPELRPNMNEVVKALEQLQESGNNEGSGISHKEPRQSPYSNS 405          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. NCBI nr
Match: gi|1366012335|ref|XP_024159098.1| (receptor-like cytoplasmic kinase 176 isoform X1 [Rosa chinensis] >gi|1366012337|ref|XP_024159099.1| receptor-like cytoplasmic kinase 176 isoform X1 [Rosa chinensis])

HSP 1 Score: 672.159 bits (1733), Expect = 0.000e+0
Identity = 342/429 (79.72%), Postives = 368/429 (85.78%), Query Frame = 0

Query:  460 MSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAAADSVDKMKAQEKSM------FSICDVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYIR 882
            MSGG+R+SVK +LNAKRL FS EIDTV+ETNE I RNF+ALQAA DSVDK K QEKS+       S+ DVPST   +  ILL+RF +NELK ATWNFHP NKLGEG FG VF+GWVDDN STAA PGT ML+AVK IN+EGLQGH+EWLTEIYYLGRL HPN+V LLGYCFEDD RLLVYEFMA GSL+ HL+G  S LQPLSWILRMKIALGAA SLAFIHS EGKVIHRD KSSNILLDSNYNAKLSDFGFAKDGPAGD SHVST VMGT GYAAPEYVATGHLT K DVYGFGVVMLELL+G+ VLD SRPTRE++LVEW++PY ASKRRVLKIFDARIKGQYSVA ARKAANL ++CLS EP LRPNMNEVVKALEQLQESGN EGSGIS  E RQS YSNSNSAKSSW+SWISFRSS S  YIR
Sbjct:    1 MSGGLRSSVKSYLNAKRLPFSGEIDTVNETNETINRNFSALQAAVDSVDKKKPQEKSIASTSYQLSMYDVPSTSR-RERILLKRFLFNELKVATWNFHPHNKLGEGGFGSVFRGWVDDNISTAASPGTGMLIAVKRINQEGLQGHKEWLTEIYYLGRLRHPNLVTLLGYCFEDDCRLLVYEFMAGGSLDTHLYGRASSLQPLSWILRMKIALGAANSLAFIHSGEGKVIHRDVKSSNILLDSNYNAKLSDFGFAKDGPAGDKSHVSTWVMGTRGYAAPEYVATGHLTRKTDVYGFGVVMLELLTGKPVLDTSRPTRERNLVEWARPYFASKRRVLKIFDARIKGQYSVAAARKAANLTNQCLSTEPELRPNMNEVVKALEQLQESGNNEGSGISHKEPRQSPYSNSNSAKSSWRSWISFRSSSSHSYIR 428          

HSP 2 Score: 426.402 bits (1095), Expect = 9.611e-138
Identity = 234/445 (52.58%), Postives = 299/445 (67.19%), Query Frame = 0

Query:   21 MGGGIRTSVKGYLLSQRITMAS--DTVHE-NESINRNFTAPLAA----DTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLF-RGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASS 453
            M GG+R+SVK YL ++R+  +   DTV+E NE+INRNF+A  AA    D ++     + ST S++ S                                Y VPS  + +  ILLK F +NELK AT NF P   LGEGGFG V++GW+D N   A+ PGTGM +A+KR++Q+ +QG +EW  E+YYLG+LRHPNLV L+GYC ED+  LLVYEF+  GSL+ +L+ R SS   ++PLSW  RMKIALGAA +LAF+H+ E ++I RD KSSNILLDS YN KLSDFG   DG +    G K+ V   ++GT GYAAPEY+ATG LT K DVYGFGVVMLELL+GK V D +RP  E+NLVEWA+PY   K RVL+IFDARI+GQ+SVA A KA +L  QCLS  P+LRPNMN VVKALE+LQ+S + +   IS  +P Q+  ++S
Sbjct:    1 MSGGLRSSVKSYLNAKRLPFSGEIDTVNETNETINRNFSALQAAVDSVDKKKPQEKSIAST-SYQLSM-------------------------------YDVPSTSRRE-RILLKRFLFNELKVATWNFHPHNKLGEGGFGSVFRGWVDDNISTAASPGTGMLIAVKRINQEGLQGHKEWLTEIYYLGRLRHPNLVTLLGYCFEDDCRLLVYEFMAGGSLDTHLYGRASS---LQPLSWILRMKIALGAANSLAFIHSGEGKVIHRDVKSSNILLDSNYNAKLSDFGFAKDGPA----GDKSHVSTWVMGTRGYAAPEYVATGHLTRKTDVYGFGVVMLELLTGKPVLDTSRPTRERNLVEWARPYFASKRRVLKIFDARIKGQYSVAAARKAANLTNQCLSTEPELRPNMNEVVKALEQLQESGNNEGSGISHKEPRQSPYSNS 405          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. NCBI nr
Match: gi|1366012339|ref|XP_024159100.1| (receptor-like cytoplasmic kinase 176 isoform X2 [Rosa chinensis])

HSP 1 Score: 667.922 bits (1722), Expect = 0.000e+0
Identity = 340/425 (80.00%), Postives = 366/425 (86.12%), Query Frame = 0

Query:  460 MSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAAADSVDKMKAQEKSMFSICD--VPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYIR 882
            MSGG+R+SVK +LNAKRL FS EIDTV+ETNE I RNF+ALQAA DSVDK K QEKS+ S     +PST   +  ILL+RF +NELK ATWNFHP NKLGEG FG VF+GWVDDN STAA PGT ML+AVK IN+EGLQGH+EWLTEIYYLGRL HPN+V LLGYCFEDD RLLVYEFMA GSL+ HL+G  S LQPLSWILRMKIALGAA SLAFIHS EGKVIHRD KSSNILLDSNYNAKLSDFGFAKDGPAGD SHVST VMGT GYAAPEYVATGHLT K DVYGFGVVMLELL+G+ VLD SRPTRE++LVEW++PY ASKRRVLKIFDARIKGQYSVA ARKAANL ++CLS EP LRPNMNEVVKALEQLQESGN EGSGIS  E RQS YSNSNSAKSSW+SWISFRSS S  YIR
Sbjct:    1 MSGGLRSSVKSYLNAKRLPFSGEIDTVNETNETINRNFSALQAAVDSVDKKKPQEKSIASTSYQLMPSTSR-RERILLKRFLFNELKVATWNFHPHNKLGEGGFGSVFRGWVDDNISTAASPGTGMLIAVKRINQEGLQGHKEWLTEIYYLGRLRHPNLVTLLGYCFEDDCRLLVYEFMAGGSLDTHLYGRASSLQPLSWILRMKIALGAANSLAFIHSGEGKVIHRDVKSSNILLDSNYNAKLSDFGFAKDGPAGDKSHVSTWVMGTRGYAAPEYVATGHLTRKTDVYGFGVVMLELLTGKPVLDTSRPTRERNLVEWARPYFASKRRVLKIFDARIKGQYSVAAARKAANLTNQCLSTEPELRPNMNEVVKALEQLQESGNNEGSGISHKEPRQSPYSNSNSAKSSWRSWISFRSSSSHSYIR 424          

HSP 2 Score: 422.55 bits (1085), Expect = 2.234e-136
Identity = 232/441 (52.61%), Postives = 296/441 (67.12%), Query Frame = 0

Query:   21 MGGGIRTSVKGYLLSQRITMAS--DTVHE-NESINRNFTAPLAADTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLF-RGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASS 453
            M GG+R+SVK YL ++R+  +   DTV+E NE+INRNF+A  AA         +DS +  +              S AS S              Y++  +      ILLK F +NELK AT NF P   LGEGGFG V++GW+D N   A+ PGTGM +A+KR++Q+ +QG +EW  E+YYLG+LRHPNLV L+GYC ED+  LLVYEF+  GSL+ +L+ R SS   ++PLSW  RMKIALGAA +LAF+H+ E ++I RD KSSNILLDS YN KLSDFG   DG +    G K+ V   ++GT GYAAPEY+ATG LT K DVYGFGVVMLELL+GK V D +RP  E+NLVEWA+PY   K RVL+IFDARI+GQ+SVA A KA +L  QCLS  P+LRPNMN VVKALE+LQ+S + +   IS  +P Q+  ++S
Sbjct:    1 MSGGLRSSVKSYLNAKRLPFSGEIDTVNETNETINRNFSALQAA---------VDSVDKKKPQE----------KSIASTS--------------YQLMPSTSRRERILLKRFLFNELKVATWNFHPHNKLGEGGFGSVFRGWVDDNISTAASPGTGMLIAVKRINQEGLQGHKEWLTEIYYLGRLRHPNLVTLLGYCFEDDCRLLVYEFMAGGSLDTHLYGRASS---LQPLSWILRMKIALGAANSLAFIHSGEGKVIHRDVKSSNILLDSNYNAKLSDFGFAKDGPA----GDKSHVSTWVMGTRGYAAPEYVATGHLTRKTDVYGFGVVMLELLTGKPVLDTSRPTRERNLVEWARPYFASKRRVLKIFDARIKGQYSVAAARKAANLTNQCLSTEPELRPNMNEVVKALEQLQESGNNEGSGISHKEPRQSPYSNS 401          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy TrEMBL
Match: A0A2P6SKH5_ROSCH (Putative transferase, protein kinase RLK-Pelle-RLCK-VIIa-2 family OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0367351 PE=4 SV=1)

HSP 1 Score: 1074.69 bits (2778), Expect = 0.000e+0
Identity = 554/833 (66.51%), Postives = 641/833 (76.95%), Query Frame = 0

Query:  107 RAESLPY----RVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSRNGI-----GTMSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAAADSVDKMKAQEKS--------------------------MFSICD----------------------------VPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISF 872
            R  SLP      V S P+T+ EILL+ FF NELK AT NF PD ++GEGGFG V+KGW+D +S AA+ PG GM +A+KR++++   G E+W  E+YYLG+LRHPNLVKL+GYC ED   LLV+EF+ RG L+N++F   S   ++PLSWN RMKI+L AA  LAFLHN E  ++ RD  SSNILLDSTYN KL+DFGL  DG +    G K+ V   ++GT+GYAAPEYIATG LT KCDVYGFGVVMLE+LSG+R  D++ P  +QNLV+WAKPYL  K RVL++FD  ++GQ+SVA ALKA +LA QCLS  P+ RPNM  VVKALE+LQ   D   L+I Q++PCQ+ +A+S N        TMSGGIRTSVKGFLNAKRLA S+EIDTVHETNE IT NF+ALQAAADSVDK KAQEKS                          +F IC                             +PS+P  Q++IL RRFFYNELKTATWNFHP N +GEG +G+VFKGW+DDNTST+AKPG+ ML+AVK I  EGL+G +EWLTEIYYLGRL HPNVVKLLGYCF+DDHRLLVYEFMA G+LE  L GWVSYLQPLSWILRMKIALGAAK+LAFIH+SEGKVIHRDFKSSNILLDSNYNAKL+DFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLT KNDVYG+GVVMLELL+G+ VLD SRPTREQDLVEW+KPYL SKRRVLKI DARIKGQYSVA ARKAANL ++CLS EP LRPNMNEVVK LEQLQESG+IEG+GIS +E  QS YSNS+S KSSW+SWISF
Sbjct:    4 RKHSLPAVSSAAVSSCPRTEGEILLRRFFLNELKMATWNFHPDNMVGEGGFGSVFKGWVDDSS-AAANPGKGMLIAVKRINKEGFLGHEQWLAEIYYLGRLRHPNLVKLLGYCFEDGHRLLVFEFMSRGGLDNHIFGRDSC--LQPLSWNLRMKISLEAANVLAFLHNGEETVVHRDITSSNILLDSTYNAKLADFGLAKDGPT----GDKSHVSTAVMGTHGYAAPEYIATGHLTAKCDVYGFGVVMLEMLSGRRSLDQSLPTVQQNLVDWAKPYLASKRRVLKVFDPCLKGQYSVAGALKAANLAKQCLSEEPEFRPNMTEVVKALEQLQGPEDMKGLKIPQNEPCQSPRANSSNRRRSTRRATMSGGIRTSVKGFLNAKRLALSAEIDTVHETNETITLNFSALQAAADSVDKKKAQEKSIASTSYGLSIYDGSFPMFNSAPKLFFLFLICITNLNIVSCTNMLQFAYLNSFCCAIEGSMLPSSPRTQNEILPRRFFYNELKTATWNFHPVNMVGEGCYGRVFKGWIDDNTSTSAKPGSGMLIAVKTIKGEGLRGQKEWLTEIYYLGRLRHPNVVKLLGYCFDDDHRLLVYEFMAKGNLEFRLVGWVSYLQPLSWILRMKIALGAAKALAFIHNSEGKVIHRDFKSSNILLDSNYNAKLADFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTSKNDVYGYGVVMLELLTGKPVLDTSRPTREQDLVEWAKPYLDSKRRVLKILDARIKGQYSVAAARKAANLPNQCLSAEPELRPNMNEVVKELEQLQESGDIEGAGISHSEPCQSPYSNSSSVKSSWRSWISF 829          

HSP 2 Score: 436.032 bits (1120), Expect = 7.500e-136
Identity = 248/505 (49.11%), Postives = 321/505 (63.56%), Query Frame = 0

Query:    1 MNDVVKALEELQESE--------------------------FSIEDMGGGIRTSVKGYLLSQRITMAS--DTVHE-NESINRNFTAPLAA----DTEQETMMDLDSTNSFRFSRFSGG-----------------------IRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQN 448
            M +VVKALE+LQ  E                               M GGIRTSVKG+L ++R+ +++  DTVHE NE+I  NF+A  AA    D ++     + ST S+  S + G                        + C  +L FA    +S    I    L    PS+P+T  EIL + FFYNELK AT NF P  ++GEG +G V+KGWID N+  +++PG+GM +A+K +  + ++G++EW  E+YYLG+LRHPN+VKL+GYC +D+  LLVYEF+ +G+LE  L    S   ++PLSW  RMKIALGAAKALAF+HN E ++I RDFKSSNILLDS YN KL+DFG   DG +       T+V +GTYGYAAPEY+ATG LT+K DVYG+GVVMLELL+GK V D +RP  EQ+LVEWAKPYL  K RVL+I DARI+GQ+SVA A KA +L  QCLS  P+LRPNMN VVK LE+LQ+S D +   IS  +PCQ+
Sbjct:  317 MTEVVKALEQLQGPEDMKGLKIPQNEPCQSPRANSSNRRRSTRRATMSGGIRTSVKGFLNAKRLALSAEIDTVHETNETITLNFSALQAAADSVDKKKAQEKSIAST-SYGLSIYDGSFPMFNSAPKLFFLFLICITNLNIVSCTNMLQFA--YLNSFCCAIEGSML----PSSPRTQNEILPRRFFYNELKTATWNFHPVNMVGEGCYGRVFKGWIDDNTSTSAKPGSGMLIAVKTIKGEGLRGQKEWLTEIYYLGRLRHPNVVKLLGYCFDDDHRLLVYEFMAKGNLEFRLVGWVS--YLQPLSWILRMKIALGAAKALAFIHNSEGKVIHRDFKSSNILLDSNYNAKLADFGFAKDGPAGDSSHVSTRV-MGTYGYAAPEYVATGHLTSKNDVYGYGVVMLELLTGKPVLDTSRPTREQDLVEWAKPYLDSKRRVLKILDARIKGQYSVAAARKAANLPNQCLSAEPELRPNMNEVVKELEQLQESGDIEGAGISHSEPCQS 811          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy TrEMBL
Match: A0A2P6SKH6_ROSCH (Putative transferase, protein kinase RLK-Pelle-RLCK-VIIa-2 family OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0367411 PE=3 SV=1)

HSP 1 Score: 712.605 bits (1838), Expect = 0.000e+0
Identity = 350/449 (77.95%), Postives = 387/449 (86.19%), Query Frame = 0

Query:   20 DMGGGIRTSVKGYLLSQRITMASDTVHENESINRNFTAPLAADTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSI-RHPGAKTKVI---------------IGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQAS 452
            DMGGGIRTSVKGYLLSQRIT ASDTVH NES NRNFTAP AAD+EQET  DLDS+NS+  SRFSG I CLPIL  ASKS+ SNGTRIRAESL +RVPS P+T+VEILLKSFF+NELK ATRNFRPD V+GEGGFG V+KGWIDQNS AA++PG GMAVAIKRL+Q+S QGREEWF+E+YYLGQLRHPNLVKLIGYCSEDNELLLVYEFVP GSLEN++FR SS+F  +PLSW  RMKIALGAAKALAFLHNEA++I+RDFK++NILLDSTYNVKLSDFGL +DGR I RHPGAKT+VI               +GTYGYAAPEYIATGRL TKCDVYGFG VMLELLSG+RVFD++R PGEQ LV+WAKPYLV K RVLQIFD+ I+GQFSVARALKAVDLAF CLSR+PKLRPNMNNVVK LEKLQ+SSD D L ISQ+K  QN   S
Sbjct:   47 DMGGGIRTSVKGYLLSQRITTASDTVHANESTNRNFTAPQAADSEQETSTDLDSSNSYWLSRFSGSIPCLPILGSASKSKVSNGTRIRAESLRHRVPSTPRTEVEILLKSFFFNELKTATRNFRPDNVVGEGGFGTVFKGWIDQNSFAAAKPGKGMAVAIKRLNQESYQGREEWFREIYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPHGSLENHIFRRSSHF--QPLSWTFRMKIALGAAKALAFLHNEAKVIYRDFKAANILLDSTYNVKLSDFGLAMDGRLIDRHPGAKTRVIGTYGCAAPKYIETRVMGTYGYAAPEYIATGRLNTKCDVYGFGAVMLELLSGRRVFDRSRLPGEQKLVKWAKPYLVSKQRVLQIFDSHIKGQFSVARALKAVDLAFLCLSRDPKLRPNMNNVVKTLEKLQESSDMDGLGISQNKRRQNPHVS 493          

HSP 2 Score: 466.077 bits (1198), Expect = 3.210e-152
Identity = 249/446 (55.83%), Postives = 303/446 (67.94%), Query Frame = 0

Query:  460 MSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAA------ADSVDKMKAQEKSMFS---IC-------------------------DVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDG------PAGDS-----------SHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQS 854
            M GGIRTSVKG+L ++R+  +S  DTVH  NE+  RNF A QAA      +  +D   +   S FS    C                          VPSTP  + +ILL+ FF+NELKTAT NF P+N +GEG FG VFKGW+D N+  AAKPG  M VA+K +N+E  QG EEW  EIYYLG+L HPN+VKL+GYC ED+  LLVYEF+ +GSLE H+F   S+ QPLSW  RMKIALGAAK+LAF+H +E KVI+RDFK++NILLDS YN KLSDFG A DG      P   +            ++ TRVMGTYGYAAPEY+ATG L  K DVYGFG VMLELLSGR+V D SR   EQ LV+W+KPYL SK+RVL+IFD+ IKGQ+SVA A KA +LA  CLS +P+LRPNMN VVK LE+LQES +++G GIS N+ RQ+
Sbjct:   48 MGGGIRTSVKGYLLSQRITTAS--DTVH-ANESTNRNFTAPQAADSEQETSTDLDSSNSYWLSRFSGSIPCLPILGSASKSKVSNGTRIRAESLRHRVPSTPRTEVEILLKSFFFNELKTATRNFRPDNVVGEGGFGTVFKGWIDQNSFAAAKPGKGMAVAIKRLNQESYQGREEWFREIYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPHGSLENHIFRRSSHFQPLSWTFRMKIALGAAKALAFLH-NEAKVIYRDFKAANILLDSTYNVKLSDFGLAMDGRLIDRHPGAKTRVIGTYGCAAPKYIETRVMGTYGYAAPEYIATGRLNTKCDVYGFGAVMLELLSGRRVFDRSRLPGEQKLVKWAKPYLVSKQRVLQIFDSHIKGQFSVARALKAVDLAFLCLSRDPKLRPNMNNVVKTLEKLQESSDMDGLGISQNKRRQN 489          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy TrEMBL
Match: A0A200PQX9_9MAGN (Protein kinase domain OS=Macleaya cordata OX=56857 GN=BVC80_9085g28 PE=4 SV=1)

HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0
Identity = 337/711 (47.40%), Postives = 468/711 (65.82%), Query Frame = 0

Query:  128 KSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNEAQ--LIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSRNGIGTMSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAAADSVDKMKAQEKSMFSICDVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQ 836
            +SF + EL +AT+NF+   ++GEGGFG VYKG +D          TG  VA+K+LDQ+S+QG +E+  EV  L  L HPNLV LIGYC++ ++ LLVYE++P GSLE++LF  SS+ E   L WN RMKIA+GAA+ L +LH +A   +I+RD KS+NILLD+ +N KLSDFGL   G    +    T+V+ GTYGY APEY  +G+LT K D+Y FGVV+LEL++G++  D  +   EQ+LV W++P+L  + + +Q+ D  ++G+F +     AV +   CL   P  RP + ++V ALE L                   A    + G     G   T        +  AFS E D   + N N        + A        ++  +  SI + P+   + +  L+  F + EL++AT NF P+  LGEG FG+VFKGW+D+ T   +K G+ M+VAVK +N E +QG EEW +E+ +LGRL HPN+VKLLGYC+ED   LLVYEFM  GSLE HLF  +S +QPLSW +R+KI +GAA+ LAF+HSSE ++I+RDFK+SNILLD ++NAKLSDFG AK+GP+G  SHV+TR+MGTYGYAAPEYVATGHL  K+DVYGFGVV+LE+L+G + LD +RP+ + +LV+W KP L  +R+++++ D R++GQY    A +AA L  +CL+ E + RP+M EVV+ALEQ+Q
Sbjct:   71 RSFTFRELASATKNFKSSNLIGEGGFGRVYKGRLD----------TGQIVAVKQLDQNSLQGNQEFIVEVLMLSLLHHPNLVNLIGYCTDGDQRLLVYEYMPMGSLEDHLFDLSSDQET--LDWNTRMKIAVGAAQGLEYLHCKANPPVIYRDLKSANILLDNDFNPKLSDFGLAKLGPVGDNTHVSTRVM-GTYGYCAPEYAMSGKLTLKSDIYSFGVVLLELITGRKAIDFTKVREEQSLVAWSRPFLRDRKKFVQLADPLLQGRFPLRCYHHAVAITSMCLQEQPNFRPLIRDIVVALEYL-------------------ASQPYKPGTHNTRGPRSTPPPSPSRREGSAFSREPDGTSKNNSN--------ENAFSGSSAKNSRFSASGSIDEFPNGQILPTPNLIV-FSFAELRSATRNFKPDTVLGEGGFGKVFKGWIDEKTYAPSKIGSGMVVAVKKLNSESMQGFEEWQSEVNFLGRLSHPNLVKLLGYCWEDKELLLVYEFMQRGSLENHLFRRISSMQPLSWSIRLKITIGAARGLAFLHSSEKQIIYRDFKASNILLDGHFNAKLSDFGLAKNGPSGGDSHVTTRIMGTYGYAAPEYVATGHLYVKSDVYGFGVVLLEMLTGLRALDTNRPSGQHNLVDWLKPSLLDRRKLIRVMDQRLEGQYPSKGAVQAAQLTLKCLAPESKNRPSMKEVVEALEQIQ 740          

HSP 2 Score: 305.064 bits (780), Expect = 2.561e-87
Identity = 147/308 (47.73%), Postives = 207/308 (67.21%), Query Frame = 0

Query:  529 IQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHS-SEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQL 835
            I +    R F + EL +AT NF   N +GEG FG+V+KG +D          T  +VAVK +++  LQG++E++ E+  L  LHHPN+V L+GYC + D RLLVYE+M  GSLE HLF   S  + L W  RMKIA+GAA+ L ++H  +   VI+RD KS+NILLD+++N KLSDFG AK GP GD++HVSTRVMGTYGY APEY  +G LT K+D+Y FGVV+LEL++GRK +D ++   EQ LV WS+P+L  +++ +++ D  ++G++ +     A  + S CL  +P  RP + ++V ALE L
Sbjct:   64 IPTSTTARSFTFRELASATKNFKSSNLIGEGGFGRVYKGRLD----------TGQIVAVKQLDQNSLQGNQEFIVEVLMLSLLHHPNLVNLIGYCTDGDQRLLVYEYMPMGSLEDHLFDLSSDQETLDWNTRMKIAVGAAQGLEYLHCKANPPVIYRDLKSANILLDNDFNPKLSDFGLAKLGPVGDNTHVSTRVMGTYGYCAPEYAMSGKLTLKSDIYSFGVVLLELITGRKAIDFTKVREEQSLVAWSRPFLRDRKKFVQLADPLLQGRFPLRCYHHAVAITSMCLQEQPNFRPLIRDIVVALEYL 361          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy TrEMBL
Match: A0A2P6Q9N6_ROSCH (Putative transferase, protein kinase RLK-Pelle-RLCK-VIIa-2 family OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0029531 PE=4 SV=1)

HSP 1 Score: 587.03 bits (1512), Expect = 0.000e+0
Identity = 297/367 (80.93%), Postives = 317/367 (86.38%), Query Frame = 0

Query:  516 SMFSICDVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSNSAKSSWKSWISFRSSVSCGYIR 882
            +  S   VPST   +  ILL+RF +NELK ATWNFHP NKLGEG FG VF+GWVDDN STAA PGT ML+AVK IN+EGLQGH+EWLTEIYYLGRL HPN+V LLGYCFEDD RLLVYEFMA GSL+ HL+G  S LQPLSWILRMKIALGAA SLAFIHS EGKVIHRD KSSNILLDSNYNAKLSDFGFAKDGPAGD SHVST VMGT GYAAPEYVATGHLT K DVYGFGVVMLELL+G+ VLD SRPTRE++LVEW++PY ASKRRVLKIFDARIKGQYSVA ARKAANL ++CLS EP LRPNMNEVVKALEQLQESGN EGSGIS  E RQS YSNSNSAKSSW+SWISFRSS S  YIR
Sbjct:  164 TTLSSVSVPSTSR-RERILLKRFLFNELKVATWNFHPHNKLGEGGFGSVFRGWVDDNISTAASPGTGMLIAVKRINQEGLQGHKEWLTEIYYLGRLRHPNLVTLLGYCFEDDCRLLVYEFMAGGSLDTHLYGRASSLQPLSWILRMKIALGAANSLAFIHSGEGKVIHRDVKSSNILLDSNYNAKLSDFGFAKDGPAGDKSHVSTWVMGTRGYAAPEYVATGHLTRKTDVYGFGVVMLELLTGKPVLDTSRPTRERNLVEWARPYFASKRRVLKIFDARIKGQYSVAAARKAANLTNQCLSTEPELRPNMNEVVKALEQLQESGNNEGSGISHKEPRQSPYSNSNSAKSSWRSWISFRSSSSHSYIR 529          

HSP 2 Score: 403.29 bits (1035), Expect = 1.449e-127
Identity = 204/344 (59.30%), Postives = 255/344 (74.13%), Query Frame = 0

Query:  115 VPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLF-RGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASS 453
            VPS  + +  ILLK F +NELK AT NF P   LGEGGFG V++GW+D N   A+ PGTGM +A+KR++Q+ +QG +EW  E+YYLG+LRHPNLV L+GYC ED+  LLVYEF+  GSL+ +L+ R SS   ++PLSW  RMKIALGAA +LAF+H+ E ++I RD KSSNILLDS YN KLSDFG   DG +    G K+ V   ++GT GYAAPEY+ATG LT K DVYGFGVVMLELL+GK V D +RP  E+NLVEWA+PY   K RVL+IFDARI+GQ+SVA A KA +L  QCLS  P+LRPNMN VVKALE+LQ+S + +   IS  +P Q+  ++S
Sbjct:  171 VPSTSRRE-RILLKRFLFNELKVATWNFHPHNKLGEGGFGSVFRGWVDDNISTAASPGTGMLIAVKRINQEGLQGHKEWLTEIYYLGRLRHPNLVTLLGYCFEDDCRLLVYEFMAGGSLDTHLYGRASS---LQPLSWILRMKIALGAANSLAFIHSGEGKVIHRDVKSSNILLDSNYNAKLSDFGFAKDGPA----GDKSHVSTWVMGTRGYAAPEYVATGHLTRKTDVYGFGVVMLELLTGKPVLDTSRPTRERNLVEWARPYFASKRRVLKIFDARIKGQYSVAAARKAANLTNQCLSTEPELRPNMNEVVKALEQLQESGNNEGSGISHKEPRQSPYSNS 506          

HSP 3 Score: 182.57 bits (462), Expect = 1.426e-45
Identity = 93/148 (62.84%), Postives = 116/148 (78.38%), Query Frame = 0

Query:  147 VLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQD---SMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRG-SSNFEVEPLSWNARMKIALGAAKALAFLH-NEAQLIFRDFKSSNILLDST 289
            +LGEG     +KGWID+ S  A++PGTGMAVAIKRL+Q    ++Q  +EW +EV  LGQL HPNLVKLIGYC +D++ LLVYEFVP+GSLEN++FR  SS F++  LSWN RMKIALGAAKALAF H +EA+ I+ DF++S ILLDS 
Sbjct:    1 MLGEGRICSFFKGWIDEQSFTATKPGTGMAVAIKRLNQQLQGTLQSHKEWMEEVNCLGQLSHPNLVKLIGYCQKDDKRLLVYEFVPKGSLENHIFRRFSSCFQI--LSWNLRMKIALGAAKALAFHHSDEAKCIYVDFETSKILLDSV 146          

HSP 4 Score: 169.859 bits (429), Expect = 2.836e-41
Identity = 85/144 (59.03%), Postives = 106/144 (73.61%), Query Frame = 0

Query:  556 LGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINRE---GLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLF-GWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDS 695
            LGEG     FKGW+D+ + TA KPGT M VA+K +N++    LQ H+EW+ E+  LG+L HPN+VKL+GYC +DD RLLVYEF+  GSLE H+F  + S  Q LSW LRMKIALGAAK+LAF HS E K I+ DF++S ILLDS
Sbjct:    2 LGEGRICSFFKGWIDEQSFTATKPGTGMAVAIKRLNQQLQGTLQSHKEWMEEVNCLGQLSHPNLVKLIGYCQKDDKRLLVYEFVPKGSLENHIFRRFSSCFQILSWNLRMKIALGAAKALAFHHSDEAKCIYVDFETSKILLDS 145          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy TrEMBL
Match: A0A371G654_MUCPR (Putative serine/threonine-protein kinase PIX13 (Fragment) OS=Mucuna pruriens OX=157652 GN=PIX13 PE=4 SV=1)

HSP 1 Score: 583.563 bits (1503), Expect = 0.000e+0
Identity = 337/786 (42.88%), Postives = 470/786 (59.80%), Query Frame = 0

Query:   98 SRDSNGTRIR-AESLPY-RVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLF-RGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDR--DDLR------ISQ----DKPCQNAQASSRNGIGTMSGGIRTSVKGFLNAKRLAFSSEIDTVH-------ETNENITRNFAALQAAADSVDKMKAQEKSMFSICDVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFE-DDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVAT-----------------------GHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQ 836
            S +S  +R+R  E  P+ ++   P       L+ F Y EL+AAT+NFR DTVLGEGGFG VYKG I          G G+  AIK+L   S QG  EW  EV +LG+L HPNLVKL+GY  ED+EL LVYEF+ RGSL+N+LF RG++   V PLSW+AR+++ +GAA+ L FLH+ + Q+I+RDFK SNILLD TY VKLSDFGL     S  H    T+V+ GT+GYAAPEYIATGRL  K DVYGFG+V++E+L+GKR+ D  R   E++L +W K  L+ + +++   DA++ G++    A K  +LA +C+  NPK+RP+M  VV+ LE+++  +++  D+ +      + Q    D  C     SS  G   +S GIR + +   N    +      +          +N N + +F   + +  S    + +++  F    +   P       LR F   EL++AT NF   +  GEG FG+V+KG +  N+      G S+ +AVK +N E LQG  EW +E+  LGRL HPN+VKLLG+  E  D   LVYEF+  GSL  HLF   + ++PL W  R+K+ +GAA+ L F+HS E K+IHRDFK SNILLD  Y AKLSDFG AK  P  D SHV+TRVMGT GYAAPEY+AT                       GHL  K+DVYGFG+V++E+L G ++ +I +      L +W +  L ++ ++    DAR+ G+Y    A + A LA RC+   P++RP+M EVV+ LE+++
Sbjct:   73 SENSQASRVRDEEEFPFGQILDAPN------LRVFTYAELRAATKNFRADTVLGEGGFGRVYKGLIR---------GEGLTFAIKKLTSGSRQGVAEWLSEVNFLGRLSHPNLVKLLGYGREDDELFLVYEFMNRGSLDNHLFGRGAT---VRPLSWDARLRVMIGAARGLNFLHSLDTQIIYRDFKPSNILLDKTYMVKLSDFGLAKSVTSPDHSHVSTQVV-GTHGYAAPEYIATGRLYVKSDVYGFGIVLVEVLTGKRIGDVMRLCQEKSLRDWLKSNLLNRRKIISTMDAKLEGRYPTKLASKVAELAHKCIQENPKVRPSMKEVVETLEQVEADNEKPVDNRKRGTRSQVEQQGQPDGVCLAVTMSSGTGSNQLSAGIRNT-RLIENDNSTSLGGSSHSGGISMFFGPSSNNNTSTSFWGSENSQAS----RVRDEGEFPCGQILDAPN------LRVFTLAELRSATKNFRDNSLTGEGGFGRVYKGLIRGNS------GESLTIAVKKLNPESLQGVAEWQSEVNILGRLSHPNLVKLLGFGREGSDCCFLVYEFVHRGSLNNHLFRRGADVRPLPWDTRLKVMIGAARGLNFLHSLEIKIIHRDFKPSNILLDKTYTAKLSDFGLAKSFPLSDQSHVTTRVMGTGGYAAPEYLATVYSSSLRPFINLLRKRDKTLNGVGHLYVKSDVYGFGIVLVEVLIGNRIKNILQQGPRMSLRDWLRSNLLNRGKIRSTMDARLDGKYPTNLASQVAQLAYRCIQENPKVRPSMKEVVETLEKIE 822          

HSP 2 Score: 262.692 bits (670), Expect = 6.930e-70
Identity = 152/382 (39.79%), Postives = 220/382 (57.59%), Query Frame = 0

Query:   98 SRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNEL-LLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIAT-----------------------GRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSR 454
            S +S  +R+R E      P     D   L + F   EL++AT+NFR +++ GEGGFG VYKG I  NS      G  + +A+K+L+ +S+QG  EW  EV  LG+L HPNLVKL+G+  E ++   LVYEFV RGSL N+LFR  +  +V PL W+ R+K+ +GAA+ L FLH+ E ++I RDFK SNILLD TY  KLSDFGL      +      T  ++GT GYAAPEY+AT                       G L  K DVYGFG+V++E+L G R+ +  +     +L +W +  L+ + ++    DAR+ G++    A +   LA++C+  NPK+RP+M  VV+ LEK++         ++ +KP  N +  +R
Sbjct:  478 SENSQASRVRDEG---EFPCGQILDAPNL-RVFTLAELRSATKNFRDNSLTGEGGFGRVYKGLIRGNS------GESLTIAVKKLNPESLQGVAEWQSEVNILGRLSHPNLVKLLGFGREGSDCCFLVYEFVHRGSLNNHLFRRGA--DVRPLPWDTRLKVMIGAARGLNFLHSLEIKIIHRDFKPSNILLDKTYTAKLSDFGLA-KSFPLSDQSHVTTRVMGTGGYAAPEYLATVYSSSLRPFINLLRKRDKTLNGVGHLYVKSDVYGFGIVLVEVLIGNRIKNILQQGPRMSLRDWLRSNLLNRGKIRSTMDARLDGKYPTNLASQVAQLAYRCIQENPKVRPSMKEVVETLEKIE---------VANEKPADNLKRVTR 837          

HSP 3 Score: 229.18 bits (583), Expect = 1.071e-58
Identity = 116/240 (48.33%), Postives = 161/240 (67.08%), Query Frame = 0

Query:  603 TEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHL---TGK---NDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQ 836
            +E+  LGRL HPN+VKLLG+  ED    LVYEFM  GSL+ HLFG  + ++PL W  R+K+ + AA+ L F+HS E K+I+RDFK SNILLD  Y  KLSDFG AK   + D SHVSTRVMGT GYAAPEYVATG++   T K   N  +     +  +L+G+++ DI R  + + L +W K  L ++R+++   DA+++G+Y    A K A LA +C+   P++RP+M EVV+ LEQ++
Sbjct:  929 SEVNLLGRLSHPNLVKLLGFGREDSELFLVYEFMHCGSLDNHLFGRGANVRPLPWDTRLKVMIEAARGLNFLHSLETKIIYRDFKPSNILLDKTYTVKLSDFGLAKTVTSPDQSHVSTRVMGTRGYAAPEYVATGNINKYTHKPFINLSWKSDKTLNRVLTGKRIDDIMRLCQGKSLRDWLKSNLLNRRKIISTMDAKLEGKYPTNLASKVAELAHKCIQENPKVRPSMKEVVETLEQIE 1168          

HSP 4 Score: 201.445 bits (511), Expect = 1.252e-49
Identity = 113/268 (42.16%), Postives = 166/268 (61.94%), Query Frame = 0

Query:  196 EVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHN-EAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRL---TTKCDV---YGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSRNG 456
            EV  LG+L HPNLVKL+G+  ED+EL LVYEF+  GSL+N+LF   +N  V PL W+ R+K+ + AA+ L FLH+ E ++I+RDFK SNILLD TY VKLSDFGL     S       T+V+ GT GYAAPEY+ATG +   T K  +   +     +  +L+GKR+ D  R    ++L +W K  L+ + +++   DA++ G++    A K  +LA +C+  NPK+RP+M  VV+ LE+++  +         +KP  N +  +R+G
Sbjct:  930 EVNLLGRLSHPNLVKLLGFGREDSELFLVYEFMHCGSLDNHLFGRGAN--VRPLPWDTRLKVMIEAARGLNFLHSLETKIIYRDFKPSNILLDKTYTVKLSDFGLAKTVTSPDQSHVSTRVM-GTRGYAAPEYVATGNINKYTHKPFINLSWKSDKTLNRVLTGKRIDDIMRLCQGKSLRDWLKSNLLNRRKIISTMDAKLEGKYPTNLASKVAELAHKCIQENPKVRPSMKEVVETLEQIEADN---------EKPVDNRKRGTRSG 1185          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy TrEMBL
Match: A0A0E0K8C3_ORYPU (Uncharacterized protein OS=Oryza punctata OX=4537 PE=4 SV=1)

HSP 1 Score: 581.637 bits (1498), Expect = 0.000e+0
Identity = 318/727 (43.74%), Postives = 448/727 (61.62%), Query Frame = 0

Query:  129 SFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNEAQ--LIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKV---IIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSS---DRDDLRISQDKPC---QNAQASSRNGIGTMSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAAADSVDKMKAQEKSMFSICDVPSTPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGN 840
            SF + +L  AT  F     +GEGGFG VYKG I+           G  VA+K+L +D +QGR E+  EV  L  L HP+LV L+G+C++ +E LLVYE++P GSLE++LF        +PL WN RM+IA+G A+ L++LHN A   +I+RD K++NILLD  Y  KLSDFGL   G      G +T V   ++GTYGY AP+Y+ +G+LT K D+Y FGV++LEL++G+R++D +RP  EQ+L+ W++P+L  K +  ++ D  + G +  +   + V ++  CL   P +RP +++VV  L  +       +R  + +S    C   Q A   SR   G        SV   ++   L    +   +H+         A  + AA +V                PS       +L    LR F   EL+  T  F PE  LGEG FG V+KGW D+ T   AK    ++VAVK +N E +QG  EW +E+ +LGRL HPN+VKLLGYC E+   LLVYEFM  GSLE HLF   ++  PLSW LR+KIA+GAA+ LAF+HSSE ++I+RDFK+SNILLD +Y  KLSDFG AK+GP    SHV+TR++GTYGYAAPEYVATGHL  K+DVY FGVV+LELL+G +  D++RP+ +Q+LVEW++PY+A  +++  + D R+ G Y    A +AA LA +CL  +P+ RP+M++VV AL++++  G 
Sbjct:   51 SFTFKDLSVATGYFNEANFIGEGGFGKVYKGKIN-----------GQMVAVKQLTRDGVQGRNEFLVEVLMLTVLNHPHLVSLVGFCAQGDERLLVYEYMPFGSLESHLF--DVPLGKQPLDWNTRMRIAVGVAEGLSYLHNVADPPIIYRDMKAANILLDEDYRPKLSDFGLAKVGPV----GDRTHVSTRVMGTYGYCAPDYVVSGKLTMKSDIYSFGVLLLELITGRRIYDASRPKPEQSLLTWSRPFLHDKRKFHRLADPALHGCYPTSALNQLVVISIMCLQDQPHVRPIISDVVIGLNHVASQPYVPERSSVSLSSPARCGSPQYAGTPSRRRAGRRFLNSVVSVHSIISCALLG--QQPSDIHQPGLEAWEQSADEKPAAAAVSGGGGGGGGREEYRRWPSGGGETGRVLDAPRLRVFTLAELRAVTRGFKPEMVLGEGGFGTVYKGWADERTLNPAKSSAGVVVAVKKLNPESVQGLHEWQSEVNFLGRLVHPNLVKLLGYCKEERELLLVYEFMPKGSLENHLFRRGAF-DPLSWSLRLKIAIGAARGLAFLHSSERQIIYRDFKASNILLDVDYTPKLSDFGLAKNGPVAGRSHVTTRIIGTYGYAAPEYVATGHLYVKSDVYCFGVVLLELLTGLRAHDLNRPSHQQNLVEWARPYIAGGKKLAGLMDPRLAGDYPPKAAARAAKLADKCLCGDPKSRPSMDDVVVALQEIESMGT 757          

HSP 2 Score: 331.643 bits (849), Expect = 2.805e-97
Identity = 167/305 (54.75%), Postives = 221/305 (72.46%), Query Frame = 0

Query:  127 LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLH-NEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQ 430
            L+ F   EL+A TR F+P+ VLGEGGFG VYKGW D+ +L  ++   G+ VA+K+L+ +S+QG  EW  EV +LG+L HPNLVKL+GYC E+ ELLLVYEF+P+GSLEN+LFR  +    +PLSW+ R+KIA+GAA+ LAFLH +E Q+I+RDFK+SNILLD  Y  KLSDFGL  +G         T+ IIGTYGYAAPEY+ATG L  K DVY FGVV+LELL+G R  D NRP  +QNLVEWA+PY+    ++  + D R+ G +    A +A  LA +CL  +PK RP+M++VV AL++++
Sbjct:  453 LRVFTLAELRAVTRGFKPEMVLGEGGFGTVYKGWADERTLNPAKSSAGVVVAVKKLNPESVQGLHEWQSEVNFLGRLVHPNLVKLLGYCKEERELLLVYEFMPKGSLENHLFRRGA---FDPLSWSLRLKIAIGAARGLAFLHSSERQIIYRDFKASNILLDVDYTPKLSDFGLAKNGPVAGRSHVTTR-IIGTYGYAAPEYVATGHLYVKSDVYCFGVVLLELLTGLRAHDLNRPSHQQNLVEWARPYIAGGKKLAGLMDPRLAGDYPPKAAARAAKLADKCLCGDPKSRPSMDDVVVALQEIE 753          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy TrEMBL
Match: A0A2P6SKG7_ROSCH (Putative transferase, protein kinase RLK-Pelle-RLCK-VIIa-2 family OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0367221 PE=3 SV=1)

HSP 1 Score: 516.153 bits (1328), Expect = 3.847e-173
Identity = 249/348 (71.55%), Postives = 289/348 (83.05%), Query Frame = 0

Query:  526 TPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLY-SNSNSAKSSWKS 868
            TP  + +IL    L+ FF+NELKTAT NF P++ +GEG FG VFKGWVD+N  TAAKPGT M++AVK +N+EGLQGH+EWLTEI YLG+L HPN+VKL+GYC EDDHRLLVYEFM  GSL+ HLF   SY QPLSW LRMKIALGAAK LAF+HS E KVI+RDFK+SNILLDS YNAKLSDFG AKDGPAGD SHVSTRVMGTYGYAAPEY+ATGHLT K+DVY FGVVMLE+LSGR+ +D +RPT E  LVEW+KPYLASKR+VL++FDARI GQYSVA+A KA NLA RCLS EP+ RP MN+VVKALEQLQE  ++EG G S NE RQ+L+ S+SN  ++  KS
Sbjct:   32 TPRTEGEILMSSNLKNFFFNELKTATRNFRPDSMVGEGGFGSVFKGWVDENALTAAKPGTGMVIAVKRLNQEGLQGHKEWLTEINYLGQLRHPNLVKLIGYCLEDDHRLLVYEFMPRGSLDNHLFRRASYFQPLSWSLRMKIALGAAKGLAFLHSDEAKVIYRDFKTSNILLDSAYNAKLSDFGLAKDGPAGDRSHVSTRVMGTYGYAAPEYMATGHLTAKSDVYSFGVVMLEMLSGRRAVDKNRPTGEHSLVEWAKPYLASKRKVLQVFDARIDGQYSVASALKAVNLAIRCLSTEPKFRPIMNDVVKALEQLQEPEDMEGPGPSQNEPRQNLHASSSNGPQNRRKS 379          

HSP 2 Score: 470.7 bits (1210), Expect = 2.129e-155
Identity = 232/362 (64.09%), Postives = 284/362 (78.45%), Query Frame = 0

Query:  119 PQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLH-NEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASSRNGIGTMSGGIRTSVKGFLNAK 475
            P+T+ EIL    LK+FF+NELK ATRNFRPD+++GEGGFG V+KGW+D+N+L A++PGTGM +A+KRL+Q+ +QG +EW  E+ YLGQLRHPNLVKLIGYC ED+  LLVYEF+PRGSL+N+LFR +S F+  PLSW+ RMKIALGAAK LAFLH +EA++I+RDFK+SNILLDS YN KLSDFGL  DG +       T+V+ GTYGYAAPEY+ATG LT K DVY FGVVMLE+LSG+R  DKNRP GE +LVEWAKPYL  K +VLQ+FDARI GQ+SVA ALKAV+LA +CLS  PK RP MN+VVKALE+LQ+  D +    SQ++P QN  ASS NG        R S+ G  NA+
Sbjct:   33 PRTEGEILMSSNLKNFFFNELKTATRNFRPDSMVGEGGFGSVFKGWVDENALTAAKPGTGMVIAVKRLNQEGLQGHKEWLTEINYLGQLRHPNLVKLIGYCLEDDHRLLVYEFMPRGSLDNHLFRRASYFQ--PLSWSLRMKIALGAAKGLAFLHSDEAKVIYRDFKTSNILLDSAYNAKLSDFGLAKDGPAGDRSHVSTRVM-GTYGYAAPEYMATGHLTAKSDVYSFGVVMLEMLSGRRAVDKNRPTGEHSLVEWAKPYLASKRKVLQVFDARIDGQYSVASALKAVNLAIRCLSTEPKFRPIMNDVVKALEQLQEPEDMEGPGPSQNEPRQNLHASSSNGPQNR----RKSMNGTSNAR 387          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy TrEMBL
Match: A0A2P6SKG9_ROSCH (Putative transferase, protein kinase RLK-Pelle-RLCK-VIIa-2 family OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0367251 PE=3 SV=1)

HSP 1 Score: 511.531 bits (1316), Expect = 4.149e-172
Identity = 250/332 (75.30%), Postives = 281/332 (84.64%), Query Frame = 0

Query:   21 MGGGIRTSVKGYLLSQRITMASDTVHENESINRNFTAPLAADTEQETMMDLDSTNSFRFSRFSGGIRCLPILSFASKSRDSNGTRIRAESLPYRVPSNPQTDVEILLKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLHNEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSI-RHPGAKTKV---------------IIGTYGYAAPEYIATGRLTTK 336
            MGGGIRTSVKGYLLSQRIT ASDTVH NES NRNFTAP AAD+EQET  DLDS+NS+  SRFSG I CLPIL  ASKSR SN TRIRAESLP+RVPS P+T+VEILLKSFF+NELK AT NFRPD V+GEGGFG V+KGW+DQNSLAA++PGTG+AVA+KRL+Q+  QGREEWF E+YYLGQLRHPNLVKLIGYCSEDNELLLVYEF+PRGSLEN++FR SS+F  +PLSW  RMKIALGAAKALAFLHNEA++I RDFK+SNILLDSTYNVK+SDFGL VD R+  RH GAKT+V               ++GTYGYAAPEY+ATG L  K
Sbjct:    1 MGGGIRTSVKGYLLSQRITTASDTVHANESTNRNFTAPQAADSEQETSTDLDSSNSYWLSRFSGSIPCLPILGSASKSRVSNRTRIRAESLPHRVPSTPRTEVEILLKSFFFNELKTATSNFRPDNVVGEGGFGTVFKGWLDQNSLAAAKPGTGIAVAVKRLNQERHQGREEWFTEIYYLGQLRHPNLVKLIGYCSEDNELLLVYEFMPRGSLENHIFRRSSHF--QPLSWTVRMKIALGAAKALAFLHNEAKVIHRDFKTSNILLDSTYNVKISDFGLAVDARAFDRHRGAKTRVKGTYGYAAPEYIETRVMGTYGYAAPEYVATGILVHK 330          

HSP 2 Score: 326.635 bits (836), Expect = 1.535e-100
Identity = 181/335 (54.03%), Postives = 215/335 (64.18%), Query Frame = 0

Query:  460 MSGGIRTSVKGFLNAKRLAFSSEIDTVHETNENITRNFAALQAA------ADSVDKMKAQEKSMFS---IC-------------------------DVPSTPPIQSDILLRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGD-----------------SSHVSTRVMGTYGYAAPEYVATGHLTGKN 743
            M GGIRTSVKG+L ++R+  +S  DTVH  NE+  RNF A QAA      +  +D   +   S FS    C                          VPSTP  + +ILL+ FF+NELKTAT NF P+N +GEG FG VFKGW+D N+  AAKPGT + VAVK +N+E  QG EEW TEIYYLG+L HPN+VKL+GYC ED+  LLVYEFM  GSLE H+F   S+ QPLSW +RMKIALGAAK+LAF+H +E KVIHRDFK+SNILLDS YN K+SDFG A D  A D                   ++ TRVMGTYGYAAPEYVATG L  KN
Sbjct:    1 MGGGIRTSVKGYLLSQRITTAS--DTVH-ANESTNRNFTAPQAADSEQETSTDLDSSNSYWLSRFSGSIPCLPILGSASKSRVSNRTRIRAESLPHRVPSTPRTEVEILLKSFFFNELKTATSNFRPDNVVGEGGFGTVFKGWLDQNSLAAAKPGTGIAVAVKRLNQERHQGREEWFTEIYYLGQLRHPNLVKLIGYCSEDNELLLVYEFMPRGSLENHIFRRSSHFQPLSWTVRMKIALGAAKALAFLH-NEAKVIHRDFKTSNILLDSTYNVKISDFGLAVDARAFDRHRGAKTRVKGTYGYAAPEYIETRVMGTYGYAAPEYVATGILVHKN 331          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy TrEMBL
Match: A0A251QKK8_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G236100 PE=3 SV=1)

HSP 1 Score: 501.901 bits (1291), Expect = 1.405e-167
Identity = 236/339 (69.62%), Postives = 286/339 (84.37%), Query Frame = 0

Query:  526 TPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVATGHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSN 860
            TP  + +IL    L+ F +NELKTAT NF P++ +GEG FG VFKGWVD+N+ TAAKPGT M++AVK +N+EGLQGH+EWLTEI YLG+LHH N+V+L+GYC EDDHR+LVYEFM  GSL+ HLF   SY QPLSW LRMKIALGAAK LAF+HS E KVI+RDFK++NILLDS YNAKLSDFG AKDGPAGD SHVSTR+MGTYGYAAPEY+ATGHLT K+DVY FGVV+LE+LSGR+ +D +RP+ E +LVEW+KPYLASKR+VL+IFDARI+GQYSV  A +A NLA RCL+VEP+ R NMN+VVKALEQLQE  ++EGSG+S N+ R + ++NS+
Sbjct:   33 TPRTEGEILQSSNLKSFAFNELKTATRNFRPDSMVGEGGFGCVFKGWVDENSLTAAKPGTGMVIAVKRLNQEGLQGHKEWLTEINYLGQLHHENLVRLIGYCLEDDHRMLVYEFMPRGSLDNHLFRRASYFQPLSWNLRMKIALGAAKGLAFLHSDEAKVIYRDFKTANILLDSTYNAKLSDFGLAKDGPAGDRSHVSTRIMGTYGYAAPEYLATGHLTAKSDVYSFGVVLLEMLSGRRAVDKNRPSGEHNLVEWAKPYLASKRKVLQIFDARIEGQYSVGGALRAVNLAIRCLAVEPKFRLNMNDVVKALEQLQEPSDMEGSGVSQNDPRPNPHANSS 371          

HSP 2 Score: 455.677 bits (1171), Expect = 1.344e-149
Identity = 220/340 (64.71%), Postives = 272/340 (80.00%), Query Frame = 0

Query:  119 PQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLH-NEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIATGRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASS 453
            P+T+ EIL    LKSF +NELK ATRNFRPD+++GEGGFG V+KGW+D+NSL A++PGTGM +A+KRL+Q+ +QG +EW  E+ YLGQL H NLV+LIGYC ED+  +LVYEF+PRGSL+N+LFR +S F+  PLSWN RMKIALGAAK LAFLH +EA++I+RDFK++NILLDSTYN KLSDFGL  DG +       T+ I+GTYGYAAPEY+ATG LT K DVY FGVV+LE+LSG+R  DKNRP GE NLVEWAKPYL  K +VLQIFDARI GQ+SV  AL+AV+LA +CL+  PK R NMN+VVKALE+LQ+ SD +   +SQ+ P  N  A+S
Sbjct:   34 PRTEGEILQSSNLKSFAFNELKTATRNFRPDSMVGEGGFGCVFKGWVDENSLTAAKPGTGMVIAVKRLNQEGLQGHKEWLTEINYLGQLHHENLVRLIGYCLEDDHRMLVYEFMPRGSLDNHLFRRASYFQ--PLSWNLRMKIALGAAKGLAFLHSDEAKVIYRDFKTANILLDSTYNAKLSDFGLAKDGPAGDRSHVSTR-IMGTYGYAAPEYLATGHLTAKSDVYSFGVVLLEMLSGRRAVDKNRPSGEHNLVEWAKPYLASKRKVLQIFDARIEGQYSVGGALRAVNLAIRCLAVEPKFRLNMNDVVKALEQLQEPSDMEGSGVSQNDPRPNPHANS 370          

BLAST of maker-Fvb7-4-snap-gene-64.46-mRNA-1 vs. ExPASy TrEMBL
Match: A0A314YJ84_PRUYE (Protein kinase APK1A chloroplastic-like OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_29749 PE=3 SV=1)

HSP 1 Score: 496.508 bits (1277), Expect = 1.886e-165
Identity = 234/340 (68.82%), Postives = 285/340 (83.82%), Query Frame = 0

Query:  526 TPPIQSDIL----LRRFFYNELKTATWNFHPENKLGEGSFGQVFKGWVDDNTSTAAKPGTSMLVAVKNINREGLQGHEEWLTEIYYLGRLHHPNVVKLLGYCFEDDHRLLVYEFMAYGSLEIHLFGWVSYLQPLSWILRMKIALGAAKSLAFIHSSEGKVIHRDFKSSNILLDSNYNAKLSDFGFAKDGPAGDSSHVSTRVMGTYGYAAPEYVAT-GHLTGKNDVYGFGVVMLELLSGRKVLDISRPTREQDLVEWSKPYLASKRRVLKIFDARIKGQYSVATARKAANLASRCLSVEPRLRPNMNEVVKALEQLQESGNIEGSGISLNELRQSLYSNSN 860
            TP  + +IL    L+ F +NELKTAT NF P++ +GEG FG VFKGW+D+N+ TAAKPGT M++AVK +N+EGLQGH+EWLTEI YLG+LHH N+V+L+GYC EDDHR+LVYEFM  GSL+ HLF   SY QPLSW LRMKIALGAAK LAF+HS E KVI+RDFK++NILLDS YNAKLSDFG AKDGPAGD SHVSTR+MGTYGYAAPEY+AT GHLT K+DVY FGVV+LE+LSGR+ +D +RP+ E +LVEW+KPYLASKR+VL+IFDARI+GQYSV  A +A NLA RCL+VE + RPNMN+V+KALEQLQE  + EGSG+S N+ R + ++NS+
Sbjct:   33 TPRTEGEILQSSNLKSFAFNELKTATRNFRPDSMVGEGGFGCVFKGWIDENSLTAAKPGTGMVIAVKRLNQEGLQGHKEWLTEINYLGQLHHENLVRLIGYCLEDDHRMLVYEFMPRGSLDNHLFRRASYFQPLSWNLRMKIALGAAKGLAFLHSDEAKVIYRDFKTANILLDSTYNAKLSDFGLAKDGPAGDRSHVSTRIMGTYGYAAPEYLATGGHLTAKSDVYSFGVVLLEMLSGRRAVDKNRPSGEHNLVEWAKPYLASKRKVLQIFDARIEGQYSVGGALRAVNLAIRCLAVESKFRPNMNDVIKALEQLQEPSDTEGSGVSQNDPRPNPHANSS 372          

HSP 2 Score: 451.44 bits (1160), Expect = 6.787e-148
Identity = 220/341 (64.52%), Postives = 272/341 (79.77%), Query Frame = 0

Query:  119 PQTDVEIL----LKSFFYNELKAATRNFRPDTVLGEGGFGPVYKGWIDQNSLAASEPGTGMAVAIKRLDQDSMQGREEWFKEVYYLGQLRHPNLVKLIGYCSEDNELLLVYEFVPRGSLENYLFRGSSNFEVEPLSWNARMKIALGAAKALAFLH-NEAQLIFRDFKSSNILLDSTYNVKLSDFGLVVDGRSIRHPGAKTKVIIGTYGYAAPEYIAT-GRLTTKCDVYGFGVVMLELLSGKRVFDKNRPPGEQNLVEWAKPYLVRKHRVLQIFDARIRGQFSVARALKAVDLAFQCLSRNPKLRPNMNNVVKALEKLQQSSDRDDLRISQDKPCQNAQASS 453
            P+T+ EIL    LKSF +NELK ATRNFRPD+++GEGGFG V+KGWID+NSL A++PGTGM +A+KRL+Q+ +QG +EW  E+ YLGQL H NLV+LIGYC ED+  +LVYEF+PRGSL+N+LFR +S F  +PLSWN RMKIALGAAK LAFLH +EA++I+RDFK++NILLDSTYN KLSDFGL  DG +       T+ I+GTYGYAAPEY+AT G LT K DVY FGVV+LE+LSG+R  DKNRP GE NLVEWAKPYL  K +VLQIFDARI GQ+SV  AL+AV+LA +CL+   K RPNMN+V+KALE+LQ+ SD +   +SQ+ P  N  A+S
Sbjct:   34 PRTEGEILQSSNLKSFAFNELKTATRNFRPDSMVGEGGFGCVFKGWIDENSLTAAKPGTGMVIAVKRLNQEGLQGHKEWLTEINYLGQLHHENLVRLIGYCLEDDHRMLVYEFMPRGSLDNHLFRRASYF--QPLSWNLRMKIALGAAKGLAFLHSDEAKVIYRDFKTANILLDSTYNAKLSDFGLAKDGPAGDRSHVSTR-IMGTYGYAAPEYLATGGHLTAKSDVYSFGVVLLEMLSGRRAVDKNRPSGEHNLVEWAKPYLASKRKVLQIFDARIEGQYSVGGALRAVNLAIRCLAVESKFRPNMNDVIKALEQLQEPSDTEGSGVSQNDPRPNPHANS 371