A new three-locus model for rootstock-induced dwarfing in apple revealed by genetic mapping of root bark percentage

Publication Overview
TitleA new three-locus model for rootstock-induced dwarfing in apple revealed by genetic mapping of root bark percentage
AuthorsHarrison N, Harrison RJ, Barber-Perez N, Cascant-Lopez E, Cobo-Medina M, Lipska M, Conde-Ruíz R, Brain P, Gregory PJ, Fernández-Fernández F
TypeJournal Article
Journal NameJournal of experimental botany
Volume67
Issue6
Year2016
Page(s)1871-1881
CitationHarrison N, Harrison RJ, Barber-Perez N, Cascant-Lopez E, Cobo-Medina M, Lipska M, Conde-Ruíz R, Brain P, Gregory PJ, Fernández-Fernández F. A new three-locus model for rootstock-induced dwarfing in apple revealed by genetic mapping of root bark percentage. Journal of experimental botany. 2016 Jan 29; 67(6):1871-1881.

Abstract

Rootstock-induced dwarfing of apple scions revolutionized global apple production during the twentieth century, leading to the development of modern intensive orchards. A high root bark percentage (the percentage of the whole root area constituted by root cortex) has previously been associated with rootstock-induced dwarfing in apple. In this study, the root bark percentage was measured in a full-sib family of ungrafted apple rootstocks and found to be under the control of three loci. Two quantitative trait loci (QTLs) for root bark percentage were found to co-localize to the same genomic regions on chromosome 5 and chromosome 11 previously identified as controlling dwarfing, Dw1 and Dw2, respectively. A third QTL was identified on chromosome 13 in a region that has not been previously associated with dwarfing. The development of closely linked sequence-tagged site markers improved the resolution of allelic classes, thereby allowing the detection of dominance and epistatic interactions between loci, with high root bark percentage only occurring in specific allelic combinations. In addition, we report a significant negative correlation between root bark percentage and stem diameter (an indicator of tree vigour), measured on a clonally propagated grafted subset of the mapping population. The demonstrated link between root bark percentage and rootstock-induced dwarfing of the scion leads us to propose a three-locus model that is able to explain levels of dwarfing from the dwarf 'M.27' to the semi-invigorating rootstock 'M.116'. Moreover, we suggest that the QTL on chromosome 13 (Rb3) might be analogous to a third dwarfing QTL, Dw3, which has not previously been identified.

Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
M27M27cultivar
M116M116cultivar
M27_x_M116M27_x_M116population
Projects
This publication contains information about 1 projects:
Project NameDescription
Apple-Dwarfing-Harrison-2016
Features
This publication contains information about 179 features:
Feature NameUniquenameType
EM_Rb1EM_Rb1genetic_marker
EM_Rb2EM_Rb2genetic_marker
EM_Rb3EM_Rb3genetic_marker
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Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Apple-M27xM116-2016
Properties
Additional details for this publication include:
Property NameValue
Copyright© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
eISSN1460-2431
Elocationerw001
ISSN1460-2431
Journal AbbreviationJ. Exp. Bot.
LanguageEnglish
Language AbbrENG
Publication Date2016 Jan 29
Publication ModelPrint-Electronic
Publication TypeJournal Article
Cross References
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DatabaseAccession
PMID: PubMedPMID:26826217