Microsatellite markers spanning the apple (Malus x domestica Borkh.) genome
Publication Overview
Abstract A new set of 148 apple microsatellite markers
has been developed and mapped on the apple reference
linkage map Fiesta x Discovery. One-hundred and
seventeen markers were developed from genomic
libraries enriched with the repeats GA, GT, AAG,
AAC and ATC; 31 were developed from EST sequences.
Markers derived from sequences containing dinucleotide
repeats were generally more polymorphic than sequences
containing trinucleotide repeats. Additional eight SSRs
from published apple, pear, and Sorbus torminalis SSRs,
whose position on the apple genome was unknown, have
also been mapped. The transferability of SSRs across
Maloideae species resulted in being efficient with 41%
of the markers successfully transferred. For all 156
SSRs, the primer sequences, repeat type, map position,
and quality of the amplification products are reported.
Also presented are allele sizes, ranges, and number of
SSRs found in a set of nine cultivars. All this
information and those of the previous CH-SSR series
can be searched at the apple SSR database (http://www.
hidras.unimi.it) to which updates and comments can be
added. A large number of apple ESTs containing SSR
repeats are available and should be used for the
development of new apple SSRs. The apple SSR
database is also meant to become an international
platform for coordinating this effort. The increased
coverage of the apple genome with SSRs allowed the
selection of a set of 86 reliable, highly polymorphic, and
overall the apple genome well-scattered SSRs. These
SSRs cover about 85% of the genome with an average
distance of one marker per 15 cM.
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