Deciphering gamma-decalactone biosynthesis in strawberry fruit using a combination of genetic mapping, RNA-Seq and eQTL analyses

Publication Overview
TitleDeciphering gamma-decalactone biosynthesis in strawberry fruit using a combination of genetic mapping, RNA-Seq and eQTL analyses
AuthorsSanchez-Sevilla JF, Crus-Rus E, Valpuesta V, Botella MA, Amaya I.
TypeJournal Article
Journal NameBMC Genomics
Volume15
Year2014
Page(s)218
CitationSanchez-Sevilla JF, Crus-Rus E, Valpuesta V, Botella MA, Amaya I.. Deciphering gamma-decalactone biosynthesis in strawberry fruit using a combination of genetic mapping, RNA-Seq and eQTL analyses. BMC Genomics. 2014; 15:218.

Abstract

Background Understanding the basis for volatile organic compound (VOC) biosynthesis and regulation is of great importance for the genetic improvement of fruit flavor. Lactones constitute an essential group of fatty acid-derived VOCs conferring peach-like aroma to a number of fruits including peach, plum, pineapple and strawberry. Early studies on lactone biosynthesis suggest that several enzymatic pathways could be responsible for the diversity of lactones, but detailed information on them remained elusive. In this study, we have integrated genetic mapping and genome-wide transcriptome analysis to investigate the molecular basis of natural variation in γ-decalactone content in strawberry fruit. Results As a result, the fatty acid desaturase FaFAD1 was identified as the gene underlying the locus at LGIII-2 that controls γ-decalactone production in ripening fruit. The FaFAD1 gene is specifically expressed in ripe fruits and its expression fully correlates with the presence of γ-decalactone in all 95 individuals of the mapping population. In addition, we show that the level of expression of FaFAH1, with similarity to cytochrome p450 hydroxylases, significantly correlates with the content of γ-decalactone in the mapping population. The analysis of expression quantitative trait loci (eQTL) suggests that the product of this gene also has a regulatory role in the biosynthetic pathway of lactones. Conclusions Altogether, this study provides mechanistic information of how the production of γ-decalactone is naturally controlled in strawberry, and proposes enzymatic activities necessary for the formation of this VOC in plants.
Projects
This publication contains information about 1 projects:
Project NameDescription
Strawberry-Decalactone_biosynthesis-Sanchez-Sevilla-2014
Features
This publication contains information about 397 features:
Feature NameUniquenameType
10855-38:A>G10855-38:A>Ggenetic_marker
11000-51:G>A11000-51:G>Agenetic_marker
11127-67:A>T11127-67:A>Tgenetic_marker
11300-32:C>T11300-32:C>Tgenetic_marker
11612-40:A>G11612-40:A>Ggenetic_marker
11764-46:A>T11764-46:A>Tgenetic_marker
12094-5:A>G12094-5:A>Ggenetic_marker
12094-6:C>G12094-6:C>Ggenetic_marker
12214-60:C>G12214-60:C>Ggenetic_marker
12468-22:C>A12468-22:C>Agenetic_marker
12468-43:C>A12468-43:C>Agenetic_marker
12496-18:T>A12496-18:T>Agenetic_marker
12541-37:T>C12541-37:T>Cgenetic_marker
13037-38:A>G13037-38:A>Ggenetic_marker
13259-38:G>T13259-38:G>Tgenetic_marker
13264-54:G>A13264-54:G>Agenetic_marker
13356-7:G>A13356-7:G>Agenetic_marker
13777-49:A>G13777-49:A>Ggenetic_marker
13809-41:T>G13809-41:T>Ggenetic_marker
13892-65:G>A13892-65:G>Agenetic_marker
14214-20:G>A14214-20:G>Agenetic_marker
14343-17:C>T14343-17:C>Tgenetic_marker
15215-12:A>T15215-12:A>Tgenetic_marker
15476-45:T>A15476-45:T>Agenetic_marker
15653-52:A>G15653-52:A>Ggenetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Strawberry-232x1392-F1-2014
Properties
Additional details for this publication include:
Property NameValue
DOI10.1186/1471-2164-15-218
URLhttp://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-218