Fine mapping of the rosy apple aphid resistance locus Dp-fl on Linkage Group 8 of the apple cultivar ‘Florina’

Publication Overview
TitleFine mapping of the rosy apple aphid resistance locus Dp-fl on Linkage Group 8 of the apple cultivar ‘Florina’
AuthorsPagliarani G., Dapena E. , Miñarro M. , Denancé C. , Lespinasse Y. , Rat-Morris E. , Troggio M. , Durel C.E. , Tartarini S.
TypeJournal Article
Journal NameTree Genetics & Genomes
Volume12
Year2016
Page(s)56
CitationPagliarani G., Dapena E. , Miñarro M. , Denancé C. , Lespinasse Y. , Rat-Morris E. , Troggio M. , Durel C.E. , Tartarini S. Fine mapping of the rosy apple aphid resistance locus Dp-fl on Linkage Group 8 of the apple cultivar ‘Florina’. Tree Genetics & Genomes 2016, 12:56

Abstract

Rosy apple aphid (Dysaphis plantaginea), is one of the major insect pests of apple, causing serious physical and economic damage to fruit production. A dominant resistance gene Dp-fl was previously mapped at the bottom of linkage group LG8 from the cultivar ‘Florina’, linked to the SSR CH01h10. The development of additional genetic markers mapping closer to Dp-fl was needed to position the gene accurately and to improve the effectiveness of marker-assisted breeding (MAB). The aims of this study were to identify single nucleotide polymorphisms (SNPs) in the region of Dp-fl and to position these SNPs relative to Dp-fl. To generate a fine map of the Dp-fl interval, a total of 191 plants segregating for resistance and derived from four different populations were tested with temperature-switch PCR (TSP) markers developed for SNPs located in the region of CH01h10. All the plants were phenotypically evaluated for aphid resistance and those data compared with the genetic data. These efforts resulted in positioning the Dp-fl resistance locus in a genetic interval corresponding to a physical distance of about 330 kb on the ‘Golden Delicious’ genome. The new markers were tested on several apple founder cultivars in order to test the specificity of the SNPs and, thus, the best markers for the MAB were identified. Finally, the 330-kb interval was analyzed for the identification of coding sequences and putative candidate genes for D. plantaginea resistance were identified.
Features
This publication contains information about 11 features:
Feature NameUniquenameType
SNP_TC_28804875SNP_TC_28804875genetic_marker
SNP_AC_30391205SNP_AC_30391205genetic_marker
SNP_GA_31139398SNP_GA_31139398genetic_marker
SNP_FB_0764494SNP_FB_0764494genetic_marker
SNP_FB_0765104SNP_FB_0765104genetic_marker
SNP_FB_0765585SNP_FB_0765585genetic_marker
SNP_FB_0766783SNP_FB_0766783genetic_marker
SNP_FB_0767171SNP_FB_0767171genetic_marker
SNP_FB_0767496SNP_FB_0767496genetic_marker
SNP_FB_0769013SNP_FB_0769013genetic_marker
resistance to Dysaphis plantaginearesistance to rosy apple aphid-Dp-flheritable_phenotypic_marker
Featuremaps
This publication contains information about 1 maps:
Map Name
Apple-PExFL-F1