QTL Analysis and Candidate Gene Mapping for the Polyphenol Content in Cider Apple

Publication Overview
TitleQTL Analysis and Candidate Gene Mapping for the Polyphenol Content in Cider Apple
AuthorsVerdu CF, Guyot S, Childebrand N, Bahut M, Celton J-M, Gaillard S, Lasserre-Zuber P, Troggio M, Guilet D, Laurens F
TypeJournal Article
Journal NamePLoS ONE
Volume9
Issue10
Year2014
Page(s)e107103
CitationVerdu CF, Guyot S, Childebrand N, Bahut M, Celton J-M, Gaillard S, Lasserre-Zuber P, Troggio M, Guilet D, Laurens F. QTL Analysis and Candidate Gene Mapping for the Polyphenol Content in Cider Apple. PLoS ONE. 2014; 9(10):e107103.

Abstract

Polyphenols have favorable antioxidant potential on human health suggesting that their high content is responsible for the beneficial effects of apple consumption. They control the quality of ciders as they predominantly account for astringency, bitterness, color and aroma. In this study, we identified QTLs controlling phenolic compound concentrations and the average polymerization degree of flavanols in a cider apple progeny. Thirty-two compounds belonging to five groups of phenolic compounds were identified and quantified by reversed phase liquid chromatography on both fruit extract and juice, over three years. The average polymerization degree of flavanols was estimated in fruit by phloroglucinolysis coupled to HPLC. Parental maps were built using SSR and SNP markers and used for the QTL analysis. Sixty-nine and 72 QTLs were detected on 14 and 11 linkage groups of the female and male maps, respectively. A majority of the QTLs identified in this study are specific to this population, while others are consistent with previous studies. This study presents for the first time in apple, QTLs for the mean polymerization degree of procyanidins, for which the mechanisms involved remains unknown to this day. Identification of candidate genes underlying major QTLs was then performed in silico and permitted the identification of 18 enzymes of the polyphenol pathway and six transcription factors involved in the apple anthocyanin regulation. New markers were designed from sequences of the most interesting candidate genes in order to confirm their co-localization with underlying QTLs by genetic mapping. Finally, the potential use of these QTLs in breeding programs is discussed.
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
KermerrienKermerriencultivar
FlorinaFlorinacultivar
PrimaPrimacultivar
X5210X5210breeding_research_material
X8402X8402breeding_research_material
X5210_x_X8402-F1X5210_x_X8402-F1population
Projects
This publication contains information about 1 projects:
Project NameDescription
Apple-polyphenol_content-Laurens-2014
Features
This publication contains information about 546 features:
Feature NameUniquenameType
CH03g12CH03g12genetic_marker
CH04g09CH04g09genetic_marker
CH05a02CH05a02genetic_marker
Ch05gf06Ch05gf06genetic_marker
CN445290CN445290genetic_marker
Hi07d11Hi07d11genetic_marker
Hi07e08Hi07e08genetic_marker
GDsnp00013GDsnp00013genetic_marker
GDsnp00014GDsnp00014genetic_marker
GDsnp00029GDsnp00029genetic_marker
GDsnp00033GDsnp00033genetic_marker
GDsnp00034GDsnp00034genetic_marker
GDsnp00035GDsnp00035genetic_marker
GDsnp00045GDsnp00045genetic_marker
GDsnp00048GDsnp00048genetic_marker
GDsnp00051GDsnp00051genetic_marker
GDsnp00058GDsnp00058genetic_marker
GDsnp00060GDsnp00060genetic_marker
GDsnp00062GDsnp00062genetic_marker
GDsnp00063GDsnp00063genetic_marker
GDsnp00069GDsnp00069genetic_marker
GDsnp00071GDsnp00071genetic_marker
GDsnp00078GDsnp00078genetic_marker
GDsnp00086GDsnp00086genetic_marker
GDsnp00087GDsnp00087genetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Apple-X5210X8402-F1-Consensus
Apple-X5210X8402-F1-X5210
Apple-X5210X8402-F1-X8402
Properties
Additional details for this publication include:
Property NameValue
URLhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0107103