Construction of SSR genetic linkage map and comparison on pears
【Objective】The genetic linkage map was constructed based on the Simple Sequence Repeat primers of pears and apples that have been published and developed from the transcriptome of pears by our team, which will be the foundation for the important trait mapping of QTL and marker-assisted selection in the future.【Method】A F1 mapping population was built on a cross between a western pear (Red Clapp Favorite) as the female parent, and an oriental pear (Mansoo) as the male parent. The SSR primers which matched the "CP" mode in the JoinMap 4.0 software in parents and four individuals by PCR application were selected and then tested in the F1 group. The separation of data was analyzed by JoinMap 4.0 software, and the parent genetic map was constructed respectively. The genetic map of the parents was integrated based on anchor points which were homology marks in the parents’ map on each linkages.【Result】909 pairs of SSR primers from different sources (526 pairs from pears and 283 pairs from apples that have been published, and 100 pairs were developed from the pear transcriptome by our team) was preliminarily screened through a PCR technique. The results showed that amplified fragments and polymorphism of SSR primers from apples were lower, but that the SSR primers from pears and developed through the transcriptome of pears were higher. The parents’ genetic linkage map was constructed based on the 207 pairs of SSR primers which conformed to the drawing. A female genetic map was constructed by 141 markers which was distributed on 17 linkage groups and spanned 757.34 cM in genome with an average distance of 5.37 cM between markers; A male genetic map was constructed by 153 markers which was distributed on 19 linkage groups and spanned 1 149.43 cM in genome with an average distance of 7.51 cM between markers.【Conclusion】The integrated genetic linkage maps of parents were constructed by different sources of SSR molecular markers, which contained 186 SSR markers and finally covered the genome 1 125.33 cM.
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